2-Deoxy-d-glucose treatment changes the Golgi apparatus architecture without blocking synthesis of complex lipids View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-04

AUTHORS

Carmen Ranftler, Claudia Meisslitzer-Ruppitsch, Herbert Stangl, Clemens Röhrl, Stefanie Fruhwürth, Josef Neumüller, Margit Pavelka, Adolf Ellinger

ABSTRACT

The classic Golgi apparatus organization, an arrangement of highly ordered cisternal stacks with tubular-vesicular membrane specializations on both sides, is the functional image of a continuous flow of contents and membranes with input, metabolization, and output in a dynamic steady state. In response to treatment with 2-deoxy-D-glucose (2-DG), which lowers the cellular ATP level by about 70% within minutes, this organization is rapidly replaced by tubular-glomerular membrane convolutes described as Golgi networks and bodies. 2-DG is a non-metabolizable glucose analogue and competitive inhibitor of glycolysis, which has become attractive in the context of therapeutic approaches for several kinds of tumors specifically targeting glycolysis in cancer. With the question of whether the functions of the Golgi apparatus in lipid synthesis would be influenced by the 2-DG-induced Golgi apparatus reorganization, we focused on lipid metabolism within the Golgi bodies. For this, we applied a fluorophore-labeled short-chain ceramide (BODIPY-Cer) in various combinations with 2-DG treatment to HepG2 cell cultures and followed uptake, enrichment and metabolization to higher ordered lipids. The cellular ATP status in each experiment was controlled with a bioluminescence assay, and the response of the Golgi apparatus was tracked by immunostaining of the trans-Golgi network protein TGN46. For electron microscopy, the fluorescent BODIPY-Cer signals were converted into electron-dense precipitates by photooxidation of diaminobenzidine (DAB); DAB precipitates labeled trans-Golgi areas in control cultures but also compartments at the periphery of the Golgi bodies formed in response to 2-DG treatment, thus indicating that concentration of ceramide takes place in spite of the Golgi apparatus reorganization. Lipid analyses by thin-layer chromatography (TLC) performed in parallel showed that BODIPY-Cer is not only concentrated in compartments of the 2-DG-induced Golgi bodies but is partly metabolized to BODIPY-sphingomyelin. Both, uptake and condensation of BODIPY-Cer and its conversion to complex lipids indicate that functions of the Golgi apparatus in the cellular lipid metabolism persist although the classic Golgi apparatus organization is abolished. More... »

PAGES

369-380

References to SciGraph publications

  • 2012. Electron Microscopy of Endocytic Pathways in CELL IMAGING TECHNIQUES
  • 2011-04. 2-Deoxy-d-glucose activates autophagy via endoplasmic reticulum stress rather than ATP depletion in CANCER CHEMOTHERAPY AND PHARMACOLOGY
  • 2007-06. The Warburg effect and its cancer therapeutic implications in JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
  • 2008-02. Principles of bioactive lipid signalling: lessons from sphingolipids in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2008-08. Electron microscopic visualization of fluorescent signals in cellular compartments and organelles by means of DAB-photoconversion in HISTOCHEMISTRY AND CELL BIOLOGY
  • 2005-11. Correlative microscopy and electron tomography of GFP through photooxidation in NATURE METHODS
  • 1998-06. Golgi-disturbing agents in HISTOCHEMISTRY AND CELL BIOLOGY
  • 2010-03. Characterization of endocytic compartments after holo-high density lipoprotein particle uptake in HepG2 cells in HISTOCHEMISTRY AND CELL BIOLOGY
  • 2007-09-06. Glycosphingolipid synthesis requires FAPP2 transfer of glucosylceramide in NATURE
  • 2014-06. Lipid landscapes and pipelines in membrane homeostasis in NATURE
  • 2002-09. Evaluation of 2-deoxy-D-glucose as a chemotherapeutic agent: mechanism of cell death in BRITISH JOURNAL OF CANCER
  • 2010. An Overview of Sphingolipid Metabolism: From Synthesis to Breakdown in SPHINGOLIPIDS AS SIGNALING AND REGULATORY MOLECULES
  • 2006-08. Glycolysis inhibition for anticancer treatment in ONCOGENE
  • 2011-02. The ceramide-enriched trans-Golgi compartments reorganize together with other parts of the Golgi apparatus in response to ATP-depletion in HISTOCHEMISTRY AND CELL BIOLOGY
  • 2013-09. Vesicular and non-vesicular transport feed distinct glycosylation pathways in the Golgi in NATURE
  • 2003-12. Molecular machinery for non-vesicular trafficking of ceramide in NATURE
  • 2006-11. 2-Deoxy-D-glucose reduces epilepsy progression by NRSF-CtBP–dependent metabolic regulation of chromatin structure in NATURE NEUROSCIENCE
  • 2012-03. FTS and 2-DG induce pancreatic cancer cell death and tumor shrinkage in mice in CELL DEATH & DISEASE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00418-014-1297-8

    DOI

    http://dx.doi.org/10.1007/s00418-014-1297-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1005026099

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/25422148


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Adenosine Triphosphate", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chromatography, Thin Layer", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Deoxyglucose", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Energy Metabolism", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Golgi Apparatus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hep G2 Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Lipogenesis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Microscopy, Electron, Transmission", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Microscopy, Fluorescence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sphingosine", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Time Factors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "trans-Golgi Network", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Medical University of Vienna", 
              "id": "https://www.grid.ac/institutes/grid.22937.3d", 
              "name": [
                "Department of Cell Biology and Ultrastructure Research, Center for Anatomy and Cell Biology, Medical University of Vienna, Schwarzspanierstr. 17, 1090, Vienna, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ranftler", 
            "givenName": "Carmen", 
            "id": "sg:person.0704343621.33", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0704343621.33"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Medical University of Vienna", 
              "id": "https://www.grid.ac/institutes/grid.22937.3d", 
              "name": [
                "Department of Cell Biology and Ultrastructure Research, Center for Anatomy and Cell Biology, Medical University of Vienna, Schwarzspanierstr. 17, 1090, Vienna, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Meisslitzer-Ruppitsch", 
            "givenName": "Claudia", 
            "id": "sg:person.0727712767.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727712767.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Medical University of Vienna", 
              "id": "https://www.grid.ac/institutes/grid.22937.3d", 
              "name": [
                "Institute of Medical Chemistry, Center for Pathobiochemistry and Genetics, Medical University of Vienna, W\u00e4hringerstr. 10, 1090, Vienna, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Stangl", 
            "givenName": "Herbert", 
            "id": "sg:person.0665440667.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665440667.22"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Medical University of Vienna", 
              "id": "https://www.grid.ac/institutes/grid.22937.3d", 
              "name": [
                "Institute of Medical Chemistry, Center for Pathobiochemistry and Genetics, Medical University of Vienna, W\u00e4hringerstr. 10, 1090, Vienna, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "R\u00f6hrl", 
            "givenName": "Clemens", 
            "id": "sg:person.01035371471.96", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035371471.96"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Medical University of Vienna", 
              "id": "https://www.grid.ac/institutes/grid.22937.3d", 
              "name": [
                "Institute of Medical Chemistry, Center for Pathobiochemistry and Genetics, Medical University of Vienna, W\u00e4hringerstr. 10, 1090, Vienna, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Fruhw\u00fcrth", 
            "givenName": "Stefanie", 
            "id": "sg:person.01103504671.16", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01103504671.16"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Medical University of Vienna", 
              "id": "https://www.grid.ac/institutes/grid.22937.3d", 
              "name": [
                "Department of Cell Biology and Ultrastructure Research, Center for Anatomy and Cell Biology, Medical University of Vienna, Schwarzspanierstr. 17, 1090, Vienna, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Neum\u00fcller", 
            "givenName": "Josef", 
            "id": "sg:person.01021320403.30", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021320403.30"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Medical University of Vienna", 
              "id": "https://www.grid.ac/institutes/grid.22937.3d", 
              "name": [
                "Department of Cell Biology and Ultrastructure Research, Center for Anatomy and Cell Biology, Medical University of Vienna, Schwarzspanierstr. 17, 1090, Vienna, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pavelka", 
            "givenName": "Margit", 
            "id": "sg:person.011634016351.73", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011634016351.73"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Medical University of Vienna", 
              "id": "https://www.grid.ac/institutes/grid.22937.3d", 
              "name": [
                "Department of Cell Biology and Ultrastructure Research, Center for Anatomy and Cell Biology, Medical University of Vienna, Schwarzspanierstr. 17, 1090, Vienna, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ellinger", 
            "givenName": "Adolf", 
            "id": "sg:person.01342731567.10", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342731567.10"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1139/o59-099", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001206449"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbalip.2007.01.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002128925"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth806", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003105899", 
              "https://doi.org/10.1038/nmeth806"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth806", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003105899", 
              "https://doi.org/10.1038/nmeth806"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature06097", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003574136", 
              "https://doi.org/10.1038/nature06097"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbalip.2013.06.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004498623"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/ana.21603", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004925182"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/ana.21603", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004925182"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/41.7.8515046", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007193395"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/41.7.8515046", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007193395"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature02188", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007297420", 
              "https://doi.org/10.1038/nature02188"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature02188", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007297420", 
              "https://doi.org/10.1038/nature02188"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nn1791", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007848330", 
              "https://doi.org/10.1038/nn1791"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nn1791", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007848330", 
              "https://doi.org/10.1038/nn1791"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-2818.2009.03220.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008460756"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.bjc.6600547", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008784368", 
              "https://doi.org/10.1038/sj.bjc.6600547"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.bjc.6600547", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008784368", 
              "https://doi.org/10.1038/sj.bjc.6600547"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00418-010-0773-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008803959", 
              "https://doi.org/10.1007/s00418-010-0773-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s004180050256", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009252923", 
              "https://doi.org/10.1007/s004180050256"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cddis.2012.24", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011574961", 
              "https://doi.org/10.1038/cddis.2012.24"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature13474", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015795285", 
              "https://doi.org/10.1038/nature13474"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbamem.2006.08.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016203414"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m113.528380", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016954090"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.113.6.1267", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017043152"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ceb.2006.06.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017183252"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10863-007-9086-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018021454", 
              "https://doi.org/10.1007/s10863-007-9086-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00280-010-1391-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019350655", 
              "https://doi.org/10.1007/s00280-010-1391-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0248-4900(91)90048-r", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020827543"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20111461", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021899157"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20111461", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021899157"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1600-0854.2008.00810.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022289041"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/emboj.2012.317", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022748560"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrm2329", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023972580", 
              "https://doi.org/10.1038/nrm2329"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00418-009-0672-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025935090", 
              "https://doi.org/10.1007/s00418-009-0672-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00418-009-0672-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025935090", 
              "https://doi.org/10.1007/s00418-009-0672-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.r600021200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027374654"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m111.228593", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027554064"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbalip.2007.04.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028205531"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-62703-056-4_22", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028404479", 
              "https://doi.org/10.1007/978-1-62703-056-4_22"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ejphar.2013.02.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031034374"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m110.150011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031433729"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bcp.2012.09.022", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031544492"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.274.41.29294", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032024996"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0360-3016(85)90117-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035651284"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(93)90638-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035751792"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/1535-7163.mct-07-0310", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036285477"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1209597", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036840626", 
              "https://doi.org/10.1038/sj.onc.1209597"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1209597", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036840626", 
              "https://doi.org/10.1038/sj.onc.1209597"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/0008-5472.can-11-0759", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037627848"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/0008-5472.can-08-2872", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037977499"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.lfs.2005.07.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038682563"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.lfs.2005.07.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038682563"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00418-008-0429-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040507009", 
              "https://doi.org/10.1007/s00418-008-0429-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00418-008-0429-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040507009", 
              "https://doi.org/10.1007/s00418-008-0429-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0608509103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045206515"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1084/jem.146.5.1332", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045930598"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4103/0973-1482.55137", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046293316"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature12423", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046576468", 
              "https://doi.org/10.1038/nature12423"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3109/10428194.2010.512966", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048546383"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ygyno.2011.04.024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049191760"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbamcr.2013.04.010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049921265"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0802781105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051528218"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-4419-6741-1_1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051883625", 
              "https://doi.org/10.1007/978-1-4419-6741-1_1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/b978-0-12-416026-2.00007-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052343941"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ajog.2009.10.883", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053257194"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/sageke.2003.39.pe27", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062439232"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.123.3191.309", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062467291"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.7112109", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062644172"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2174/138920112799095338", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069178019"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1074544607", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075708570", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/jnci/21.3.475", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1075847335"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1076977085", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077320775", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1078621373", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080752184", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2015-04", 
        "datePublishedReg": "2015-04-01", 
        "description": "The classic Golgi apparatus organization, an arrangement of highly ordered cisternal stacks with tubular-vesicular membrane specializations on both sides, is the functional image of a continuous flow of contents and membranes with input, metabolization, and output in a dynamic steady state. In response to treatment with 2-deoxy-D-glucose (2-DG), which lowers the cellular ATP level by about 70% within minutes, this organization is rapidly replaced by tubular-glomerular membrane convolutes described as Golgi networks and bodies. 2-DG is a non-metabolizable glucose analogue and competitive inhibitor of glycolysis, which has become attractive in the context of therapeutic approaches for several kinds of tumors specifically targeting glycolysis in cancer. With the question of whether the functions of the Golgi apparatus in lipid synthesis would be influenced by the 2-DG-induced Golgi apparatus reorganization, we focused on lipid metabolism within the Golgi bodies. For this, we applied a fluorophore-labeled short-chain ceramide (BODIPY-Cer) in various combinations with 2-DG treatment to HepG2 cell cultures and followed uptake, enrichment and metabolization to higher ordered lipids. The cellular ATP status in each experiment was controlled with a bioluminescence assay, and the response of the Golgi apparatus was tracked by immunostaining of the trans-Golgi network protein TGN46. For electron microscopy, the fluorescent BODIPY-Cer signals were converted into electron-dense precipitates by photooxidation of diaminobenzidine (DAB); DAB precipitates labeled trans-Golgi areas in control cultures but also compartments at the periphery of the Golgi bodies formed in response to 2-DG treatment, thus indicating that concentration of ceramide takes place in spite of the Golgi apparatus reorganization. Lipid analyses by thin-layer chromatography (TLC) performed in parallel showed that BODIPY-Cer is not only concentrated in compartments of the 2-DG-induced Golgi bodies but is partly metabolized to BODIPY-sphingomyelin. Both, uptake and condensation of BODIPY-Cer and its conversion to complex lipids indicate that functions of the Golgi apparatus in the cellular lipid metabolism persist although the classic Golgi apparatus organization is abolished.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s00418-014-1297-8", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1294817", 
            "issn": [
              "0948-6143", 
              "1432-119X"
            ], 
            "name": "Histochemistry and Cell Biology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "4", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "143"
          }
        ], 
        "name": "2-Deoxy-d-glucose treatment changes the Golgi apparatus architecture without blocking synthesis of complex lipids", 
        "pagination": "369-380", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "17f2ea6f06a0b72ae53ab740568ee35110a026ca128287ef79f1723f08127b98"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "25422148"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9506663"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00418-014-1297-8"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1005026099"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00418-014-1297-8", 
          "https://app.dimensions.ai/details/publication/pub.1005026099"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T14:09", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000510.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs00418-014-1297-8"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00418-014-1297-8'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00418-014-1297-8'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00418-014-1297-8'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00418-014-1297-8'


     

    This table displays all metadata directly associated to this object as RDF triples.

    378 TRIPLES      21 PREDICATES      107 URIs      34 LITERALS      22 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00418-014-1297-8 schema:about N144ae874d407407b8a174683c82bc527
    2 N18f91e61146c482e9a4734b17b50ffa0
    3 N3a9828465ef74a17981168f7c1475a38
    4 N47b7e2809ec8473385cc77a2de814769
    5 N5c507798a3824acfa81d9e9d46bd872b
    6 Nc671242f9a624c50b63529c36d37ca24
    7 Ncde1cad528264b80ab9c400635879654
    8 Nd56584b98bda40bc94dea2b872658482
    9 Ndd21f0ca4b8c48e1a86988e89d577c4a
    10 Nf35f767801f6422c903b1029f28c6b16
    11 Nf7b62e8312ff4436acc01c403c7d0de2
    12 Nfa0f52ab85664932a5c841dda114d575
    13 Nfc0f8be49ecb4ab58e671f8a072d0ee9
    14 anzsrc-for:06
    15 anzsrc-for:0601
    16 schema:author N5f09d5ee28e74f1ba62cc3037d8680db
    17 schema:citation sg:pub.10.1007/978-1-4419-6741-1_1
    18 sg:pub.10.1007/978-1-62703-056-4_22
    19 sg:pub.10.1007/s00280-010-1391-0
    20 sg:pub.10.1007/s00418-008-0429-4
    21 sg:pub.10.1007/s00418-009-0672-3
    22 sg:pub.10.1007/s00418-010-0773-z
    23 sg:pub.10.1007/s004180050256
    24 sg:pub.10.1007/s10863-007-9086-x
    25 sg:pub.10.1038/cddis.2012.24
    26 sg:pub.10.1038/nature02188
    27 sg:pub.10.1038/nature06097
    28 sg:pub.10.1038/nature12423
    29 sg:pub.10.1038/nature13474
    30 sg:pub.10.1038/nmeth806
    31 sg:pub.10.1038/nn1791
    32 sg:pub.10.1038/nrm2329
    33 sg:pub.10.1038/sj.bjc.6600547
    34 sg:pub.10.1038/sj.onc.1209597
    35 https://app.dimensions.ai/details/publication/pub.1074544607
    36 https://app.dimensions.ai/details/publication/pub.1075708570
    37 https://app.dimensions.ai/details/publication/pub.1076977085
    38 https://app.dimensions.ai/details/publication/pub.1077320775
    39 https://app.dimensions.ai/details/publication/pub.1078621373
    40 https://app.dimensions.ai/details/publication/pub.1080752184
    41 https://doi.org/10.1002/ana.21603
    42 https://doi.org/10.1016/0092-8674(93)90638-7
    43 https://doi.org/10.1016/0248-4900(91)90048-r
    44 https://doi.org/10.1016/0360-3016(85)90117-8
    45 https://doi.org/10.1016/b978-0-12-416026-2.00007-8
    46 https://doi.org/10.1016/j.ajog.2009.10.883
    47 https://doi.org/10.1016/j.bbalip.2007.01.009
    48 https://doi.org/10.1016/j.bbalip.2007.04.004
    49 https://doi.org/10.1016/j.bbalip.2013.06.006
    50 https://doi.org/10.1016/j.bbamcr.2013.04.010
    51 https://doi.org/10.1016/j.bbamem.2006.08.004
    52 https://doi.org/10.1016/j.bcp.2012.09.022
    53 https://doi.org/10.1016/j.ceb.2006.06.011
    54 https://doi.org/10.1016/j.ejphar.2013.02.005
    55 https://doi.org/10.1016/j.lfs.2005.07.001
    56 https://doi.org/10.1016/j.ygyno.2011.04.024
    57 https://doi.org/10.1038/emboj.2012.317
    58 https://doi.org/10.1042/bj20111461
    59 https://doi.org/10.1073/pnas.0608509103
    60 https://doi.org/10.1073/pnas.0802781105
    61 https://doi.org/10.1074/jbc.274.41.29294
    62 https://doi.org/10.1074/jbc.m110.150011
    63 https://doi.org/10.1074/jbc.m111.228593
    64 https://doi.org/10.1074/jbc.m113.528380
    65 https://doi.org/10.1074/jbc.r600021200
    66 https://doi.org/10.1083/jcb.113.6.1267
    67 https://doi.org/10.1084/jem.146.5.1332
    68 https://doi.org/10.1093/jnci/21.3.475
    69 https://doi.org/10.1111/j.1365-2818.2009.03220.x
    70 https://doi.org/10.1111/j.1600-0854.2008.00810.x
    71 https://doi.org/10.1126/sageke.2003.39.pe27
    72 https://doi.org/10.1126/science.123.3191.309
    73 https://doi.org/10.1126/science.7112109
    74 https://doi.org/10.1139/o59-099
    75 https://doi.org/10.1158/0008-5472.can-08-2872
    76 https://doi.org/10.1158/0008-5472.can-11-0759
    77 https://doi.org/10.1158/1535-7163.mct-07-0310
    78 https://doi.org/10.1177/41.7.8515046
    79 https://doi.org/10.2174/138920112799095338
    80 https://doi.org/10.3109/10428194.2010.512966
    81 https://doi.org/10.4103/0973-1482.55137
    82 schema:datePublished 2015-04
    83 schema:datePublishedReg 2015-04-01
    84 schema:description The classic Golgi apparatus organization, an arrangement of highly ordered cisternal stacks with tubular-vesicular membrane specializations on both sides, is the functional image of a continuous flow of contents and membranes with input, metabolization, and output in a dynamic steady state. In response to treatment with 2-deoxy-D-glucose (2-DG), which lowers the cellular ATP level by about 70% within minutes, this organization is rapidly replaced by tubular-glomerular membrane convolutes described as Golgi networks and bodies. 2-DG is a non-metabolizable glucose analogue and competitive inhibitor of glycolysis, which has become attractive in the context of therapeutic approaches for several kinds of tumors specifically targeting glycolysis in cancer. With the question of whether the functions of the Golgi apparatus in lipid synthesis would be influenced by the 2-DG-induced Golgi apparatus reorganization, we focused on lipid metabolism within the Golgi bodies. For this, we applied a fluorophore-labeled short-chain ceramide (BODIPY-Cer) in various combinations with 2-DG treatment to HepG2 cell cultures and followed uptake, enrichment and metabolization to higher ordered lipids. The cellular ATP status in each experiment was controlled with a bioluminescence assay, and the response of the Golgi apparatus was tracked by immunostaining of the trans-Golgi network protein TGN46. For electron microscopy, the fluorescent BODIPY-Cer signals were converted into electron-dense precipitates by photooxidation of diaminobenzidine (DAB); DAB precipitates labeled trans-Golgi areas in control cultures but also compartments at the periphery of the Golgi bodies formed in response to 2-DG treatment, thus indicating that concentration of ceramide takes place in spite of the Golgi apparatus reorganization. Lipid analyses by thin-layer chromatography (TLC) performed in parallel showed that BODIPY-Cer is not only concentrated in compartments of the 2-DG-induced Golgi bodies but is partly metabolized to BODIPY-sphingomyelin. Both, uptake and condensation of BODIPY-Cer and its conversion to complex lipids indicate that functions of the Golgi apparatus in the cellular lipid metabolism persist although the classic Golgi apparatus organization is abolished.
    85 schema:genre research_article
    86 schema:inLanguage en
    87 schema:isAccessibleForFree false
    88 schema:isPartOf N139e2ea03ee34b878dade110e5a42f04
    89 Nc3c149e1322c4d228aeb444a1a2d5c1b
    90 sg:journal.1294817
    91 schema:name 2-Deoxy-d-glucose treatment changes the Golgi apparatus architecture without blocking synthesis of complex lipids
    92 schema:pagination 369-380
    93 schema:productId N0f490d07ff0f4026b256b97e0c224f30
    94 N31c8848f68a745e8ba6ff448e4e1c611
    95 N8128fe350a0e45809a54bc2efdd361e6
    96 Na0c00beb1d3a4ef482510a8ad6a9189f
    97 Nf81b361757384458922f40af720b6b00
    98 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005026099
    99 https://doi.org/10.1007/s00418-014-1297-8
    100 schema:sdDatePublished 2019-04-10T14:09
    101 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    102 schema:sdPublisher N0875bd89eecc44e4ae60f049d4adb144
    103 schema:url http://link.springer.com/10.1007%2Fs00418-014-1297-8
    104 sgo:license sg:explorer/license/
    105 sgo:sdDataset articles
    106 rdf:type schema:ScholarlyArticle
    107 N02a4ed9db379494d90a9d81da9ca8a2c rdf:first sg:person.01103504671.16
    108 rdf:rest Nf2fde7205e30473a8b88a5a510d9badc
    109 N0875bd89eecc44e4ae60f049d4adb144 schema:name Springer Nature - SN SciGraph project
    110 rdf:type schema:Organization
    111 N0f490d07ff0f4026b256b97e0c224f30 schema:name dimensions_id
    112 schema:value pub.1005026099
    113 rdf:type schema:PropertyValue
    114 N139e2ea03ee34b878dade110e5a42f04 schema:volumeNumber 143
    115 rdf:type schema:PublicationVolume
    116 N144ae874d407407b8a174683c82bc527 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    117 schema:name Golgi Apparatus
    118 rdf:type schema:DefinedTerm
    119 N18f91e61146c482e9a4734b17b50ffa0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Hep G2 Cells
    121 rdf:type schema:DefinedTerm
    122 N31c8848f68a745e8ba6ff448e4e1c611 schema:name nlm_unique_id
    123 schema:value 9506663
    124 rdf:type schema:PropertyValue
    125 N3a9828465ef74a17981168f7c1475a38 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Humans
    127 rdf:type schema:DefinedTerm
    128 N45252dfd060340feb1550de14caf7b25 rdf:first sg:person.011634016351.73
    129 rdf:rest N51a99c38b53d4200b1656c0bf9924ac1
    130 N461cb8c62c9748cc890d2066d05fc331 rdf:first sg:person.0727712767.32
    131 rdf:rest N5a15a7ac2a00405fbab7849322d17a69
    132 N47b7e2809ec8473385cc77a2de814769 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Time Factors
    134 rdf:type schema:DefinedTerm
    135 N51a99c38b53d4200b1656c0bf9924ac1 rdf:first sg:person.01342731567.10
    136 rdf:rest rdf:nil
    137 N5a15a7ac2a00405fbab7849322d17a69 rdf:first sg:person.0665440667.22
    138 rdf:rest N8c54a73faaa741a4b2ef7985b611dd53
    139 N5c507798a3824acfa81d9e9d46bd872b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    140 schema:name Adenosine Triphosphate
    141 rdf:type schema:DefinedTerm
    142 N5f09d5ee28e74f1ba62cc3037d8680db rdf:first sg:person.0704343621.33
    143 rdf:rest N461cb8c62c9748cc890d2066d05fc331
    144 N8128fe350a0e45809a54bc2efdd361e6 schema:name pubmed_id
    145 schema:value 25422148
    146 rdf:type schema:PropertyValue
    147 N8c54a73faaa741a4b2ef7985b611dd53 rdf:first sg:person.01035371471.96
    148 rdf:rest N02a4ed9db379494d90a9d81da9ca8a2c
    149 Na0c00beb1d3a4ef482510a8ad6a9189f schema:name readcube_id
    150 schema:value 17f2ea6f06a0b72ae53ab740568ee35110a026ca128287ef79f1723f08127b98
    151 rdf:type schema:PropertyValue
    152 Nc3c149e1322c4d228aeb444a1a2d5c1b schema:issueNumber 4
    153 rdf:type schema:PublicationIssue
    154 Nc671242f9a624c50b63529c36d37ca24 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    155 schema:name Microscopy, Fluorescence
    156 rdf:type schema:DefinedTerm
    157 Ncde1cad528264b80ab9c400635879654 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    158 schema:name Lipogenesis
    159 rdf:type schema:DefinedTerm
    160 Nd56584b98bda40bc94dea2b872658482 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    161 schema:name trans-Golgi Network
    162 rdf:type schema:DefinedTerm
    163 Ndd21f0ca4b8c48e1a86988e89d577c4a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    164 schema:name Energy Metabolism
    165 rdf:type schema:DefinedTerm
    166 Nf2fde7205e30473a8b88a5a510d9badc rdf:first sg:person.01021320403.30
    167 rdf:rest N45252dfd060340feb1550de14caf7b25
    168 Nf35f767801f6422c903b1029f28c6b16 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    169 schema:name Sphingosine
    170 rdf:type schema:DefinedTerm
    171 Nf7b62e8312ff4436acc01c403c7d0de2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    172 schema:name Deoxyglucose
    173 rdf:type schema:DefinedTerm
    174 Nf81b361757384458922f40af720b6b00 schema:name doi
    175 schema:value 10.1007/s00418-014-1297-8
    176 rdf:type schema:PropertyValue
    177 Nfa0f52ab85664932a5c841dda114d575 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    178 schema:name Microscopy, Electron, Transmission
    179 rdf:type schema:DefinedTerm
    180 Nfc0f8be49ecb4ab58e671f8a072d0ee9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    181 schema:name Chromatography, Thin Layer
    182 rdf:type schema:DefinedTerm
    183 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    184 schema:name Biological Sciences
    185 rdf:type schema:DefinedTerm
    186 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    187 schema:name Biochemistry and Cell Biology
    188 rdf:type schema:DefinedTerm
    189 sg:journal.1294817 schema:issn 0948-6143
    190 1432-119X
    191 schema:name Histochemistry and Cell Biology
    192 rdf:type schema:Periodical
    193 sg:person.01021320403.30 schema:affiliation https://www.grid.ac/institutes/grid.22937.3d
    194 schema:familyName Neumüller
    195 schema:givenName Josef
    196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021320403.30
    197 rdf:type schema:Person
    198 sg:person.01035371471.96 schema:affiliation https://www.grid.ac/institutes/grid.22937.3d
    199 schema:familyName Röhrl
    200 schema:givenName Clemens
    201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035371471.96
    202 rdf:type schema:Person
    203 sg:person.01103504671.16 schema:affiliation https://www.grid.ac/institutes/grid.22937.3d
    204 schema:familyName Fruhwürth
    205 schema:givenName Stefanie
    206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01103504671.16
    207 rdf:type schema:Person
    208 sg:person.011634016351.73 schema:affiliation https://www.grid.ac/institutes/grid.22937.3d
    209 schema:familyName Pavelka
    210 schema:givenName Margit
    211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011634016351.73
    212 rdf:type schema:Person
    213 sg:person.01342731567.10 schema:affiliation https://www.grid.ac/institutes/grid.22937.3d
    214 schema:familyName Ellinger
    215 schema:givenName Adolf
    216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342731567.10
    217 rdf:type schema:Person
    218 sg:person.0665440667.22 schema:affiliation https://www.grid.ac/institutes/grid.22937.3d
    219 schema:familyName Stangl
    220 schema:givenName Herbert
    221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665440667.22
    222 rdf:type schema:Person
    223 sg:person.0704343621.33 schema:affiliation https://www.grid.ac/institutes/grid.22937.3d
    224 schema:familyName Ranftler
    225 schema:givenName Carmen
    226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0704343621.33
    227 rdf:type schema:Person
    228 sg:person.0727712767.32 schema:affiliation https://www.grid.ac/institutes/grid.22937.3d
    229 schema:familyName Meisslitzer-Ruppitsch
    230 schema:givenName Claudia
    231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727712767.32
    232 rdf:type schema:Person
    233 sg:pub.10.1007/978-1-4419-6741-1_1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051883625
    234 https://doi.org/10.1007/978-1-4419-6741-1_1
    235 rdf:type schema:CreativeWork
    236 sg:pub.10.1007/978-1-62703-056-4_22 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028404479
    237 https://doi.org/10.1007/978-1-62703-056-4_22
    238 rdf:type schema:CreativeWork
    239 sg:pub.10.1007/s00280-010-1391-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019350655
    240 https://doi.org/10.1007/s00280-010-1391-0
    241 rdf:type schema:CreativeWork
    242 sg:pub.10.1007/s00418-008-0429-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040507009
    243 https://doi.org/10.1007/s00418-008-0429-4
    244 rdf:type schema:CreativeWork
    245 sg:pub.10.1007/s00418-009-0672-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025935090
    246 https://doi.org/10.1007/s00418-009-0672-3
    247 rdf:type schema:CreativeWork
    248 sg:pub.10.1007/s00418-010-0773-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1008803959
    249 https://doi.org/10.1007/s00418-010-0773-z
    250 rdf:type schema:CreativeWork
    251 sg:pub.10.1007/s004180050256 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009252923
    252 https://doi.org/10.1007/s004180050256
    253 rdf:type schema:CreativeWork
    254 sg:pub.10.1007/s10863-007-9086-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1018021454
    255 https://doi.org/10.1007/s10863-007-9086-x
    256 rdf:type schema:CreativeWork
    257 sg:pub.10.1038/cddis.2012.24 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011574961
    258 https://doi.org/10.1038/cddis.2012.24
    259 rdf:type schema:CreativeWork
    260 sg:pub.10.1038/nature02188 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007297420
    261 https://doi.org/10.1038/nature02188
    262 rdf:type schema:CreativeWork
    263 sg:pub.10.1038/nature06097 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003574136
    264 https://doi.org/10.1038/nature06097
    265 rdf:type schema:CreativeWork
    266 sg:pub.10.1038/nature12423 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046576468
    267 https://doi.org/10.1038/nature12423
    268 rdf:type schema:CreativeWork
    269 sg:pub.10.1038/nature13474 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015795285
    270 https://doi.org/10.1038/nature13474
    271 rdf:type schema:CreativeWork
    272 sg:pub.10.1038/nmeth806 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003105899
    273 https://doi.org/10.1038/nmeth806
    274 rdf:type schema:CreativeWork
    275 sg:pub.10.1038/nn1791 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007848330
    276 https://doi.org/10.1038/nn1791
    277 rdf:type schema:CreativeWork
    278 sg:pub.10.1038/nrm2329 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023972580
    279 https://doi.org/10.1038/nrm2329
    280 rdf:type schema:CreativeWork
    281 sg:pub.10.1038/sj.bjc.6600547 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008784368
    282 https://doi.org/10.1038/sj.bjc.6600547
    283 rdf:type schema:CreativeWork
    284 sg:pub.10.1038/sj.onc.1209597 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036840626
    285 https://doi.org/10.1038/sj.onc.1209597
    286 rdf:type schema:CreativeWork
    287 https://app.dimensions.ai/details/publication/pub.1074544607 schema:CreativeWork
    288 https://app.dimensions.ai/details/publication/pub.1075708570 schema:CreativeWork
    289 https://app.dimensions.ai/details/publication/pub.1076977085 schema:CreativeWork
    290 https://app.dimensions.ai/details/publication/pub.1077320775 schema:CreativeWork
    291 https://app.dimensions.ai/details/publication/pub.1078621373 schema:CreativeWork
    292 https://app.dimensions.ai/details/publication/pub.1080752184 schema:CreativeWork
    293 https://doi.org/10.1002/ana.21603 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004925182
    294 rdf:type schema:CreativeWork
    295 https://doi.org/10.1016/0092-8674(93)90638-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035751792
    296 rdf:type schema:CreativeWork
    297 https://doi.org/10.1016/0248-4900(91)90048-r schema:sameAs https://app.dimensions.ai/details/publication/pub.1020827543
    298 rdf:type schema:CreativeWork
    299 https://doi.org/10.1016/0360-3016(85)90117-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035651284
    300 rdf:type schema:CreativeWork
    301 https://doi.org/10.1016/b978-0-12-416026-2.00007-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052343941
    302 rdf:type schema:CreativeWork
    303 https://doi.org/10.1016/j.ajog.2009.10.883 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053257194
    304 rdf:type schema:CreativeWork
    305 https://doi.org/10.1016/j.bbalip.2007.01.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002128925
    306 rdf:type schema:CreativeWork
    307 https://doi.org/10.1016/j.bbalip.2007.04.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028205531
    308 rdf:type schema:CreativeWork
    309 https://doi.org/10.1016/j.bbalip.2013.06.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004498623
    310 rdf:type schema:CreativeWork
    311 https://doi.org/10.1016/j.bbamcr.2013.04.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049921265
    312 rdf:type schema:CreativeWork
    313 https://doi.org/10.1016/j.bbamem.2006.08.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016203414
    314 rdf:type schema:CreativeWork
    315 https://doi.org/10.1016/j.bcp.2012.09.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031544492
    316 rdf:type schema:CreativeWork
    317 https://doi.org/10.1016/j.ceb.2006.06.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017183252
    318 rdf:type schema:CreativeWork
    319 https://doi.org/10.1016/j.ejphar.2013.02.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031034374
    320 rdf:type schema:CreativeWork
    321 https://doi.org/10.1016/j.lfs.2005.07.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038682563
    322 rdf:type schema:CreativeWork
    323 https://doi.org/10.1016/j.ygyno.2011.04.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049191760
    324 rdf:type schema:CreativeWork
    325 https://doi.org/10.1038/emboj.2012.317 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022748560
    326 rdf:type schema:CreativeWork
    327 https://doi.org/10.1042/bj20111461 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021899157
    328 rdf:type schema:CreativeWork
    329 https://doi.org/10.1073/pnas.0608509103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045206515
    330 rdf:type schema:CreativeWork
    331 https://doi.org/10.1073/pnas.0802781105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051528218
    332 rdf:type schema:CreativeWork
    333 https://doi.org/10.1074/jbc.274.41.29294 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032024996
    334 rdf:type schema:CreativeWork
    335 https://doi.org/10.1074/jbc.m110.150011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031433729
    336 rdf:type schema:CreativeWork
    337 https://doi.org/10.1074/jbc.m111.228593 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027554064
    338 rdf:type schema:CreativeWork
    339 https://doi.org/10.1074/jbc.m113.528380 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016954090
    340 rdf:type schema:CreativeWork
    341 https://doi.org/10.1074/jbc.r600021200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027374654
    342 rdf:type schema:CreativeWork
    343 https://doi.org/10.1083/jcb.113.6.1267 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017043152
    344 rdf:type schema:CreativeWork
    345 https://doi.org/10.1084/jem.146.5.1332 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045930598
    346 rdf:type schema:CreativeWork
    347 https://doi.org/10.1093/jnci/21.3.475 schema:sameAs https://app.dimensions.ai/details/publication/pub.1075847335
    348 rdf:type schema:CreativeWork
    349 https://doi.org/10.1111/j.1365-2818.2009.03220.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1008460756
    350 rdf:type schema:CreativeWork
    351 https://doi.org/10.1111/j.1600-0854.2008.00810.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022289041
    352 rdf:type schema:CreativeWork
    353 https://doi.org/10.1126/sageke.2003.39.pe27 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062439232
    354 rdf:type schema:CreativeWork
    355 https://doi.org/10.1126/science.123.3191.309 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062467291
    356 rdf:type schema:CreativeWork
    357 https://doi.org/10.1126/science.7112109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062644172
    358 rdf:type schema:CreativeWork
    359 https://doi.org/10.1139/o59-099 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001206449
    360 rdf:type schema:CreativeWork
    361 https://doi.org/10.1158/0008-5472.can-08-2872 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037977499
    362 rdf:type schema:CreativeWork
    363 https://doi.org/10.1158/0008-5472.can-11-0759 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037627848
    364 rdf:type schema:CreativeWork
    365 https://doi.org/10.1158/1535-7163.mct-07-0310 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036285477
    366 rdf:type schema:CreativeWork
    367 https://doi.org/10.1177/41.7.8515046 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007193395
    368 rdf:type schema:CreativeWork
    369 https://doi.org/10.2174/138920112799095338 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069178019
    370 rdf:type schema:CreativeWork
    371 https://doi.org/10.3109/10428194.2010.512966 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048546383
    372 rdf:type schema:CreativeWork
    373 https://doi.org/10.4103/0973-1482.55137 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046293316
    374 rdf:type schema:CreativeWork
    375 https://www.grid.ac/institutes/grid.22937.3d schema:alternateName Medical University of Vienna
    376 schema:name Department of Cell Biology and Ultrastructure Research, Center for Anatomy and Cell Biology, Medical University of Vienna, Schwarzspanierstr. 17, 1090, Vienna, Austria
    377 Institute of Medical Chemistry, Center for Pathobiochemistry and Genetics, Medical University of Vienna, Währingerstr. 10, 1090, Vienna, Austria
    378 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...