Ontology type: schema:ScholarlyArticle
2008-08-05
AUTHORSKazunori Sango, Hiroko Yanagisawa, Yukari Komuta, Yang Si, Hitoshi Kawano
ABSTRACTWe observed that recombinant ciliary neurotrophic factor (CNTF) enhanced survival and neurite outgrowth of cultured adult rat dorsal root ganglion (DRG) neurons. Among other neurotrophic factors (NGF and GDNF) and interleukin (IL)-6 cytokine members [IL-6, LIF, cardiotrophin-1, and oncostatin M (OSM)] at the same concentration (50 ng/ml), CNTF, as well as LIF and OSM, displayed high efficacy for the promotion of the number of viable neurons and neurite-bearing cells. CNTF enhanced the number of neurite-bearing cells in both small neurons (soma diameter <30 μm) and large neurons (soma diameter ≥30 μm), whereas NGF and GDNF promoted that in only small neurons. Western blot analysis revealed that CNTF induced phosphorylation of STAT3, Akt, and ERK1/2 in the neurons. Furthermore, the neurite outgrowth-promoting activity of CNTF was diminished by co-treatment with Janus kinase (JAK) 2 inhibitor, AG490; STAT3 inhibitor, STA-21; phosphatidyl inositol-3′-phosphate-kinase (PI3K) inhibitor, LY294002; and mitogen-activated protein kinase kinase (MEK) inhibitor, PD98059, in a concentration-dependent manner. Its survival-promoting activity was also affected by AG490, STA-21, and LY294002 at higher concentrations, but not by PD98059. These findings suggest the involvement of JAK2/STAT3, PI3K/Akt, and MEK/ERK signaling pathways in CNTF-induced neurite outgrowth, where the former two pathways are thought to play major roles in mediating the survival response of neurons to CNTF. More... »
PAGES669-679
http://scigraph.springernature.com/pub.10.1007/s00418-008-0484-x
DOIhttp://dx.doi.org/10.1007/s00418-008-0484-x
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1024683471
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/18679704
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Survival",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cells, Cultured",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Chromones",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Ciliary Neurotrophic Factor",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Dose-Response Relationship, Drug",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Female",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Flavonoids",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Ganglia, Spinal",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Immunohistochemistry",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mitogen-Activated Protein Kinases",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Morpholines",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Neurons",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Neuroprotective Agents",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Phosphorylation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Polycyclic Compounds",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Proto-Oncogene Proteins c-akt",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Rats",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Rats, Wistar",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "STAT3 Transcription Factor",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Signal Transduction",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Tyrphostins",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Division of Neural Development and Regeneration, Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, 183-8526, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.272456.0",
"name": [
"Division of Neural Development and Regeneration, Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, 183-8526, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Sango",
"givenName": "Kazunori",
"id": "sg:person.0737564767.12",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0737564767.12"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Neural Development and Regeneration, Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, 183-8526, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.272456.0",
"name": [
"Division of Neural Development and Regeneration, Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, 183-8526, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Yanagisawa",
"givenName": "Hiroko",
"id": "sg:person.0744057267.04",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744057267.04"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Neural Development and Regeneration, Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, 183-8526, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.272456.0",
"name": [
"Division of Neural Development and Regeneration, Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, 183-8526, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Komuta",
"givenName": "Yukari",
"id": "sg:person.01321220777.12",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321220777.12"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Neural Development and Regeneration, Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, 183-8526, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.272456.0",
"name": [
"Division of Neural Development and Regeneration, Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, 183-8526, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Si",
"givenName": "Yang",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Neural Development and Regeneration, Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, 183-8526, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.272456.0",
"name": [
"Division of Neural Development and Regeneration, Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, 183-8526, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Kawano",
"givenName": "Hitoshi",
"id": "sg:person.01204772377.08",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204772377.08"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/35046558",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018577936",
"https://doi.org/10.1038/35046558"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-0-387-30381-9_3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020270470",
"https://doi.org/10.1007/978-0-387-30381-9_3"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00418-007-0290-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002395218",
"https://doi.org/10.1007/s00418-007-0290-x"
],
"type": "CreativeWork"
}
],
"datePublished": "2008-08-05",
"datePublishedReg": "2008-08-05",
"description": "We observed that recombinant ciliary neurotrophic factor (CNTF) enhanced survival and neurite outgrowth of cultured adult rat dorsal root ganglion (DRG) neurons. Among other neurotrophic factors (NGF and GDNF) and interleukin (IL)-6 cytokine members [IL-6, LIF, cardiotrophin-1, and oncostatin M (OSM)] at the same concentration (50\u00a0ng/ml), CNTF, as well as LIF and OSM, displayed high efficacy for the promotion of the number of viable neurons and neurite-bearing cells. CNTF enhanced the number of neurite-bearing cells in both small neurons (soma diameter <30\u00a0\u03bcm) and large neurons (soma diameter \u226530\u00a0\u03bcm), whereas NGF and GDNF promoted that in only small neurons. Western blot analysis revealed that CNTF induced phosphorylation of STAT3, Akt, and ERK1/2 in the neurons. Furthermore, the neurite outgrowth-promoting activity of CNTF was diminished by co-treatment with Janus kinase (JAK) 2 inhibitor, AG490; STAT3 inhibitor, STA-21; phosphatidyl inositol-3\u2032-phosphate-kinase (PI3K) inhibitor, LY294002; and mitogen-activated protein kinase kinase (MEK) inhibitor, PD98059, in a concentration-dependent manner. Its survival-promoting activity was also affected by AG490, STA-21, and LY294002 at higher concentrations, but not by PD98059. These findings suggest the involvement of JAK2/STAT3, PI3K/Akt, and MEK/ERK signaling pathways in CNTF-induced neurite outgrowth, where the former two pathways are thought to play major roles in mediating the survival response of neurons to CNTF.",
"genre": "article",
"id": "sg:pub.10.1007/s00418-008-0484-x",
"inLanguage": "en",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1294817",
"issn": [
"0948-6143",
"1432-119X"
],
"name": "Histochemistry and Cell Biology",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "4",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "130"
}
],
"keywords": [
"neurite-bearing cells",
"ciliary neurotrophic factor",
"cultured adult rat dorsal root ganglion neurons",
"mitogen-activated protein kinase kinase inhibitor",
"protein kinase kinase inhibitor",
"neurite outgrowth",
"kinase kinase inhibitor",
"MEK/ERK",
"kinase inhibitors",
"STA-21",
"phosphorylation of STAT3",
"PI3K/Akt",
"Janus kinase 2 inhibitor",
"kinase 2 inhibitor",
"neurite outgrowth-promoting activity",
"JAK2/STAT3",
"survival-promoting activity",
"Western blot analysis",
"outgrowth-promoting activity",
"STAT3 inhibitor",
"survival response",
"recombinant ciliary neurotrophic factor",
"blot analysis",
"dorsal root ganglion neurons",
"PD98059",
"LY294002",
"concentration-dependent manner",
"Akt",
"STAT3",
"pathway",
"inhibitors",
"outgrowth",
"rat dorsal root ganglion neurons",
"neurotrophic factor",
"ganglion neurons",
"cells",
"major role",
"phosphorylation",
"adult rat dorsal root ganglion neurons",
"ERK",
"AG490",
"ERK1/2",
"neurons",
"phosphatidyl",
"activity",
"high concentrations",
"cytokine member",
"same concentration",
"GDNF",
"NGF",
"members",
"factors",
"role",
"survival",
"manner",
"large neurons",
"OSM",
"number",
"response",
"viable neurons",
"small neurons",
"concentration",
"involvement",
"analysis",
"neuroprotective properties",
"high efficacy",
"interleukin",
"findings",
"promotion",
"LiF",
"properties",
"efficacy"
],
"name": "Neuroprotective properties of ciliary neurotrophic factor for cultured adult rat dorsal root ganglion neurons",
"pagination": "669-679",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1024683471"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s00418-008-0484-x"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"18679704"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s00418-008-0484-x",
"https://app.dimensions.ai/details/publication/pub.1024683471"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-20T07:25",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_469.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s00418-008-0484-x"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00418-008-0484-x'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00418-008-0484-x'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00418-008-0484-x'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00418-008-0484-x'
This table displays all metadata directly associated to this object as RDF triples.
261 TRIPLES
22 PREDICATES
123 URIs
112 LITERALS
29 BLANK NODES