The effect of the enkephalin DADLE on transcription does not depend on opioid receptors View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-08

AUTHORS

Beatrice Baldelli, Lorella Vecchio, Maria Grazia Bottone, Giovanni Muzzonigro, Marco Biggiogera, Manuela Malatesta

ABSTRACT

[D-Ala(2),D-Leu(5)] enkephalin (DADLE) is a synthetic peptide capable of inducing a hibernation-like state in mammals in vivo and in cultured cells in vitro. The effects of DADLE seem to be due to its binding to opioid receptors; however, it inhibits the growth of LNCaP cells, devoid of opioid receptors. We have investigated the effects of DADLE on this cell line using transmission electron microscopy, immunocytochemistry and cytometry, in order to elucidate the general mechanism(s) by which this enkephalin affects cell metabolism. We demonstrated that, similar to cell lines provided with opioid receptors, in LNCaP cells DADLE induces structural modifications of cytoplasmic and nuclear constituents, as well as a decrease in transcription and proliferation. However, DADLE does not provoke an increase in apoptotic or necrotic cell fraction, and, after removing the enkephalin from the culture medium, all effects disappear. We also demonstrated that DADLE molecules enter the cytoplasm and the nucleus of LNCaP cells, mostly binding to perichromatin fibrils and dense fibrillar component, where transcription and early splicing of pre-mRNAs and pre-rRNAs occur. In conclusion, our data demonstrate that the effect of DADLE on transcription and on cultured cells does not depend on opioid receptors. DADLE can, therefore, be envisaged as an extremely promising molecule to be used for inducing a reversible hypometabolic state in various cultured cells, without provoking cell damage or death. More... »

PAGES

189-197

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00418-006-0145-x

DOI

http://dx.doi.org/10.1007/s00418-006-0145-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052186380

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16416021


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curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00418-006-0145-x'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00418-006-0145-x'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00418-006-0145-x'


 

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