Ontology type: schema:ScholarlyArticle
2006-08
AUTHORSBeatrice Baldelli, Lorella Vecchio, Maria Grazia Bottone, Giovanni Muzzonigro, Marco Biggiogera, Manuela Malatesta
ABSTRACT[D-Ala(2),D-Leu(5)] enkephalin (DADLE) is a synthetic peptide capable of inducing a hibernation-like state in mammals in vivo and in cultured cells in vitro. The effects of DADLE seem to be due to its binding to opioid receptors; however, it inhibits the growth of LNCaP cells, devoid of opioid receptors. We have investigated the effects of DADLE on this cell line using transmission electron microscopy, immunocytochemistry and cytometry, in order to elucidate the general mechanism(s) by which this enkephalin affects cell metabolism. We demonstrated that, similar to cell lines provided with opioid receptors, in LNCaP cells DADLE induces structural modifications of cytoplasmic and nuclear constituents, as well as a decrease in transcription and proliferation. However, DADLE does not provoke an increase in apoptotic or necrotic cell fraction, and, after removing the enkephalin from the culture medium, all effects disappear. We also demonstrated that DADLE molecules enter the cytoplasm and the nucleus of LNCaP cells, mostly binding to perichromatin fibrils and dense fibrillar component, where transcription and early splicing of pre-mRNAs and pre-rRNAs occur. In conclusion, our data demonstrate that the effect of DADLE on transcription and on cultured cells does not depend on opioid receptors. DADLE can, therefore, be envisaged as an extremely promising molecule to be used for inducing a reversible hypometabolic state in various cultured cells, without provoking cell damage or death. More... »
PAGES189-197
http://scigraph.springernature.com/pub.10.1007/s00418-006-0145-x
DOIhttp://dx.doi.org/10.1007/s00418-006-0145-x
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1052186380
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/16416021
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Death",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Line, Tumor",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Nucleus",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Proliferation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cytoplasm",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Enkephalin, Leucine-2-Alanine",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Immunohistochemistry",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Microscopy, Electron, Transmission",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "RNA, Messenger",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Receptors, Opioid",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Transcription, Genetic",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Urbino",
"id": "https://www.grid.ac/institutes/grid.12711.34",
"name": [
"Istituto di Istologia e Analisi di Laboratorio, University of Urbino \u201cCarlo Bo\u201d, 61029, Urbino, Italy"
],
"type": "Organization"
},
"familyName": "Baldelli",
"givenName": "Beatrice",
"id": "sg:person.01306004113.45",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306004113.45"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Pavia",
"id": "https://www.grid.ac/institutes/grid.8982.b",
"name": [
"Laboratorio di Biologia Cellulare e Neurobiologia, Dipartimento di Biologia Animale, Universit\u00e0 di Pavia, Pavia, Italy"
],
"type": "Organization"
},
"familyName": "Vecchio",
"givenName": "Lorella",
"id": "sg:person.0670326327.84",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0670326327.84"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Pavia",
"id": "https://www.grid.ac/institutes/grid.8982.b",
"name": [
"Laboratorio di Biologia Cellulare e Neurobiologia, Dipartimento di Biologia Animale, Universit\u00e0 di Pavia, Pavia, Italy",
"Istituto di Genetica Molecolare del CNR (Sezione di Istochimica e Citometria), Universit\u00e0 di Pavia, 27100, Pavia, Italy"
],
"type": "Organization"
},
"familyName": "Bottone",
"givenName": "Maria Grazia",
"id": "sg:person.01027647571.74",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01027647571.74"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Marche Polytechnic University",
"id": "https://www.grid.ac/institutes/grid.7010.6",
"name": [
"Istituto di Scienze Materno Infantili, Cattedra di Clinica Urologica, University Politecnica delle Marche, 60100, Ancona, Italy"
],
"type": "Organization"
},
"familyName": "Muzzonigro",
"givenName": "Giovanni",
"id": "sg:person.0767463167.47",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0767463167.47"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Pavia",
"id": "https://www.grid.ac/institutes/grid.8982.b",
"name": [
"Laboratorio di Biologia Cellulare e Neurobiologia, Dipartimento di Biologia Animale, Universit\u00e0 di Pavia, Pavia, Italy",
"Istituto di Genetica Molecolare del CNR (Sezione di Istochimica e Citometria), Universit\u00e0 di Pavia, 27100, Pavia, Italy"
],
"type": "Organization"
},
"familyName": "Biggiogera",
"givenName": "Marco",
"id": "sg:person.01110135410.05",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110135410.05"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Urbino",
"id": "https://www.grid.ac/institutes/grid.12711.34",
"name": [
"Istituto di Istologia e Analisi di Laboratorio, University of Urbino \u201cCarlo Bo\u201d, 61029, Urbino, Italy"
],
"type": "Organization"
},
"familyName": "Malatesta",
"givenName": "Manuela",
"id": "sg:person.0735555401.07",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735555401.07"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/bf00347694",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001386510",
"https://doi.org/10.1007/bf00347694"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jemt.20077",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001775187"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1161/01.cir.100.suppl_2.ii-357",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008584837"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jemt.1070270406",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009410662"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/cbir.2000.0575",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012910290"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/(sici)1097-0185(199711)249:3<359::aid-ar6>3.0.co;2-p",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016275974"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/ndt/14.8.1982",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023455161"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s004180050437",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024865990",
"https://doi.org/10.1007/s004180050437"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00418-005-0085-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027094742",
"https://doi.org/10.1007/s00418-005-0085-x"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00418-005-0085-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027094742",
"https://doi.org/10.1007/s00418-005-0085-x"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0091-3057(97)00063-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027975103"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0014-2999(01)01346-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029778815"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1042/bj20031059",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031544092"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1042/bj20031059",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031544092"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0003-4975(96)00108-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032299924"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0040816602000745",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034082940"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0040816602000745",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034082940"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/cyto.990140416",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036469850"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1159/000025448",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036551079",
"https://doi.org/10.1159/000025448"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/(sici)1097-0185(19990301)254:3<389::aid-ar10>3.0.co;2-e",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041697279"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0091-3057(95)02153-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042757634"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1161/01.cir.99.23.3079",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043567773"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0024-3205(88)90406-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043952172"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0024-3205(88)90406-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043952172"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0022-5320(69)80016-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044705657"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02255466",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046047880",
"https://doi.org/10.1007/bf02255466"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02255466",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046047880",
"https://doi.org/10.1007/bf02255466"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0003-4975(97)00631-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046054061"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.biolcel.2004.04.013",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047671298"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0014-2999(97)01213-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050209438"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0305-0491(98)00056-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051151786"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s004410051082",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051606801",
"https://doi.org/10.1007/s004410051082"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s004410051082",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051606801",
"https://doi.org/10.1007/s004410051082"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0091-3057(97)00062-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052362817"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s004120100166",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053734833",
"https://doi.org/10.1007/s004120100166"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1096/fasebj.14.5.828",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1074614948"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1074659718",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1075579362",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1076839875",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1078895409",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079063312",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1081983028",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082219130",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082552845",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082742392",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1523/jneurosci.13-01-00104.1993",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1082800807"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1083361638",
"type": "CreativeWork"
}
],
"datePublished": "2006-08",
"datePublishedReg": "2006-08-01",
"description": "[D-Ala(2),D-Leu(5)] enkephalin (DADLE) is a synthetic peptide capable of inducing a hibernation-like state in mammals in vivo and in cultured cells in vitro. The effects of DADLE seem to be due to its binding to opioid receptors; however, it inhibits the growth of LNCaP cells, devoid of opioid receptors. We have investigated the effects of DADLE on this cell line using transmission electron microscopy, immunocytochemistry and cytometry, in order to elucidate the general mechanism(s) by which this enkephalin affects cell metabolism. We demonstrated that, similar to cell lines provided with opioid receptors, in LNCaP cells DADLE induces structural modifications of cytoplasmic and nuclear constituents, as well as a decrease in transcription and proliferation. However, DADLE does not provoke an increase in apoptotic or necrotic cell fraction, and, after removing the enkephalin from the culture medium, all effects disappear. We also demonstrated that DADLE molecules enter the cytoplasm and the nucleus of LNCaP cells, mostly binding to perichromatin fibrils and dense fibrillar component, where transcription and early splicing of pre-mRNAs and pre-rRNAs occur. In conclusion, our data demonstrate that the effect of DADLE on transcription and on cultured cells does not depend on opioid receptors. DADLE can, therefore, be envisaged as an extremely promising molecule to be used for inducing a reversible hypometabolic state in various cultured cells, without provoking cell damage or death.",
"genre": "research_article",
"id": "sg:pub.10.1007/s00418-006-0145-x",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1294817",
"issn": [
"0948-6143",
"1432-119X"
],
"name": "Histochemistry and Cell Biology",
"type": "Periodical"
},
{
"issueNumber": "2",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "126"
}
],
"name": "The effect of the enkephalin DADLE on transcription does not depend on opioid receptors",
"pagination": "189-197",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"f8949ac4c7acac9733f8735c74e12942249a419486790bea90eec7da6627aaac"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"16416021"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"9506663"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s00418-006-0145-x"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1052186380"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s00418-006-0145-x",
"https://app.dimensions.ai/details/publication/pub.1052186380"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T12:44",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000363_0000000363/records_70066_00000002.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007%2Fs00418-006-0145-x"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00418-006-0145-x'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00418-006-0145-x'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00418-006-0145-x'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00418-006-0145-x'
This table displays all metadata directly associated to this object as RDF triples.
279 TRIPLES
21 PREDICATES
82 URIs
33 LITERALS
21 BLANK NODES