Fluorescently labeled inhibitors detect localized serine protease activities in Drosophila melanogaster pole cells, embryos, and ovarian egg chambers View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-01

AUTHORS

Rasmus Kragh Jakobsen, Shin Ono, James C. Powers, Robert DeLotto

ABSTRACT

Serine proteases are typically synthesized as proteolytically inactive zymogens that often become activated in a limited and highly localized manner. Consequently, determination of the spatial and temporal activation pattern of these molecules is of great importance to understanding the biological processes that they mediate. Until only recently, the tools to conveniently address the question of where and when serine proteases are active within complex tissues have been lacking. In order to detect spatially restricted serine protease activities in Drosophila embryos and ovaries we introduce a technique using fluorescent synthetic and protein-based inhibitors. With this approach we have detected a novel serine protease activity with a relative mobility of 37 kDa, localized to the surface of pole cells, the germ-line precursors, in embryos between nuclear cycles 11 and 14 in development. A second novel cell-specific protease activity was localized to the tissues of early gastrulating embryos. Microinjection of inhibitors into the perivitelline space of stage 2 embryos perturbed normal embryonic development. Fluorescein-conjugated chymotrypsin inhibitor and Bowman-Birk inhibitor labeled protease activity localized to the oocyte-somatic follicle cell interface of the developing egg chamber. Our results suggest that this technique holds promise to identify new spatially restricted activities in adult Drosophila tissues and developing embryos. More... »

PAGES

51-60

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00418-004-0734-5

DOI

http://dx.doi.org/10.1007/s00418-004-0734-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026434577

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15609041


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Division", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Movement", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Nucleus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drosophila melanogaster", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryo, Nonmammalian", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescein", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescent Dyes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gastrula", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ovary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ovum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Serine Endopeptidases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Serine Proteinase Inhibitors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Signal Transduction", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Copenhagen", 
          "id": "https://www.grid.ac/institutes/grid.5254.6", 
          "name": [
            "Department of Genetics, Institute of Molecular Biology, University of Copenhagen, \u00d8ster Farimagsgade\u00a02A, 1353, Copenhagen\u00a0K, Denmark"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jakobsen", 
        "givenName": "Rasmus Kragh", 
        "id": "sg:person.01216462212.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216462212.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Toyama", 
          "id": "https://www.grid.ac/institutes/grid.267346.2", 
          "name": [
            "Department of System Engineering of Materials and Life Science, Faculty of Engineering, Toyama University, Gofuku 3190, 930-8555, Toyama, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ono", 
        "givenName": "Shin", 
        "id": "sg:person.015305143715.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015305143715.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Georgia Institute of Technology", 
          "id": "https://www.grid.ac/institutes/grid.213917.f", 
          "name": [
            "Department of Chemistry and Biochemistry, Georgia Institute of Technology, GA\u00a030332-0400, Atlanta, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Powers", 
        "givenName": "James C.", 
        "id": "sg:person.01016560766.59", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016560766.59"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Copenhagen", 
          "id": "https://www.grid.ac/institutes/grid.5254.6", 
          "name": [
            "Department of Genetics, Institute of Molecular Biology, University of Copenhagen, \u00d8ster Farimagsgade\u00a02A, 1353, Copenhagen\u00a0K, Denmark"
          ], 
          "type": "Organization"
        }, 
        "familyName": "DeLotto", 
        "givenName": "Robert", 
        "id": "sg:person.0652240346.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652240346.27"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1093/emboj/cdg224", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000579518"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-9861(61)90145-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000992256"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0962-8924(92)90246-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002097545"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0012-1606(80)90173-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002575091"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1004983365", 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-662-02454-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004983365", 
          "https://doi.org/10.1007/978-3-662-02454-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-662-02454-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004983365", 
          "https://doi.org/10.1007/978-3-662-02454-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0065-1281(96)80041-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005887409"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(93)90393-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008112299"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cr010182v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009807257"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cr010182v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009807257"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0012-1606(89)90225-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010035548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.240320105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015630520"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00291041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017141140", 
          "https://doi.org/10.1007/bf00291041"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.287.5461.2185", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019737566"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/358387a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022067404", 
          "https://doi.org/10.1038/358387a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.081026598", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022195361"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0012-1606(83)90025-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024139559"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/20.10.2387", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025169740"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.cb.05.110189.001521", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031452008"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0065-2776(01)79005-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031480596"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0091-679x(08)60931-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037017189"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1529-0131(200109)44:9<2084::aid-art359>3.0.co;2-r", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041494312"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/047084664x.ch11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045430921"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0925-4773(91)90019-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047936194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0925-4773(91)90019-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047936194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(92)90181-b", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051670923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(91)90381-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054549985"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bc00029a004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055153016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074795416", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076372774", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0889-8588(18)30282-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1076575764"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1159/000468898", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077639318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078471759", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081907007", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2005-01", 
    "datePublishedReg": "2005-01-01", 
    "description": "Serine proteases are typically synthesized as proteolytically inactive zymogens that often become activated in a limited and highly localized manner. Consequently, determination of the spatial and temporal activation pattern of these molecules is of great importance to understanding the biological processes that they mediate. Until only recently, the tools to conveniently address the question of where and when serine proteases are active within complex tissues have been lacking. In order to detect spatially restricted serine protease activities in Drosophila embryos and ovaries we introduce a technique using fluorescent synthetic and protein-based inhibitors. With this approach we have detected a novel serine protease activity with a relative mobility of 37 kDa, localized to the surface of pole cells, the germ-line precursors, in embryos between nuclear cycles 11 and 14 in development. A second novel cell-specific protease activity was localized to the tissues of early gastrulating embryos. Microinjection of inhibitors into the perivitelline space of stage 2 embryos perturbed normal embryonic development. Fluorescein-conjugated chymotrypsin inhibitor and Bowman-Birk inhibitor labeled protease activity localized to the oocyte-somatic follicle cell interface of the developing egg chamber. Our results suggest that this technique holds promise to identify new spatially restricted activities in adult Drosophila tissues and developing embryos.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00418-004-0734-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2515035", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2516760", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1294817", 
        "issn": [
          "0948-6143", 
          "1432-119X"
        ], 
        "name": "Histochemistry and Cell Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "123"
      }
    ], 
    "name": "Fluorescently labeled inhibitors detect localized serine protease activities in Drosophila melanogaster pole cells, embryos, and ovarian egg chambers", 
    "pagination": "51-60", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "7deef0bbbceccea6c3d3fc4e253f678a62e55f5e0249f5044ff2331169547b4c"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "15609041"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9506663"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00418-004-0734-5"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1026434577"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00418-004-0734-5", 
      "https://app.dimensions.ai/details/publication/pub.1026434577"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T14:26", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000373_0000000373/records_13069_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00418-004-0734-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00418-004-0734-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00418-004-0734-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00418-004-0734-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00418-004-0734-5'


 

This table displays all metadata directly associated to this object as RDF triples.

258 TRIPLES      21 PREDICATES      77 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00418-004-0734-5 schema:about N2c43b3748a324d73872939da74ba8493
2 N3f090afd614e436097ad6171304039c6
3 N46a37b0afc3540779bb7b3ad46139f34
4 N5eccbee1871247bbad9509a57ea2773e
5 N6825e9ecb2614dd7830fdad0cfe1dd95
6 N8432b89550a94db5894cd20072fe86ad
7 N88cc083e495c4d20af36513b9ce9c118
8 N97d46cb71b8047b4aa69d8ea4c248769
9 N9aa6f039b30a44fa9511720efb752716
10 Na7bdba17827d4bea887b0a5c2b275894
11 Nc9655c86a7e54a8ca7feb9c9e8db41db
12 Nca7b73e8eb724bf28eb9df5e7410899c
13 Nd59343801e324589ab10c7f74ff6e4bd
14 Nd9abb9d781e349169f50b11e3c141be9
15 Ne2132253595f4431aa64693f9cbccd22
16 Ned9e400665cb44c2961ffd9d6158049c
17 anzsrc-for:06
18 anzsrc-for:0601
19 schema:author Na5780ddbc9994e7f942a416e2de23738
20 schema:citation sg:pub.10.1007/978-3-662-02454-6
21 sg:pub.10.1007/bf00291041
22 sg:pub.10.1038/358387a0
23 https://app.dimensions.ai/details/publication/pub.1004983365
24 https://app.dimensions.ai/details/publication/pub.1074795416
25 https://app.dimensions.ai/details/publication/pub.1076372774
26 https://app.dimensions.ai/details/publication/pub.1078471759
27 https://app.dimensions.ai/details/publication/pub.1081907007
28 https://doi.org/10.1002/047084664x.ch11
29 https://doi.org/10.1002/1529-0131(200109)44:9<2084::aid-art359>3.0.co;2-r
30 https://doi.org/10.1002/jcb.240320105
31 https://doi.org/10.1016/0003-9861(61)90145-x
32 https://doi.org/10.1016/0012-1606(80)90173-6
33 https://doi.org/10.1016/0012-1606(83)90025-8
34 https://doi.org/10.1016/0012-1606(89)90225-x
35 https://doi.org/10.1016/0092-8674(91)90381-8
36 https://doi.org/10.1016/0092-8674(92)90181-b
37 https://doi.org/10.1016/0092-8674(93)90393-5
38 https://doi.org/10.1016/0925-4773(91)90019-3
39 https://doi.org/10.1016/0962-8924(92)90246-j
40 https://doi.org/10.1016/s0065-1281(96)80041-9
41 https://doi.org/10.1016/s0065-2776(01)79005-0
42 https://doi.org/10.1016/s0091-679x(08)60931-0
43 https://doi.org/10.1016/s0889-8588(18)30282-x
44 https://doi.org/10.1021/bc00029a004
45 https://doi.org/10.1021/cr010182v
46 https://doi.org/10.1073/pnas.081026598
47 https://doi.org/10.1093/emboj/20.10.2387
48 https://doi.org/10.1093/emboj/cdg224
49 https://doi.org/10.1126/science.287.5461.2185
50 https://doi.org/10.1146/annurev.cb.05.110189.001521
51 https://doi.org/10.1159/000468898
52 schema:datePublished 2005-01
53 schema:datePublishedReg 2005-01-01
54 schema:description Serine proteases are typically synthesized as proteolytically inactive zymogens that often become activated in a limited and highly localized manner. Consequently, determination of the spatial and temporal activation pattern of these molecules is of great importance to understanding the biological processes that they mediate. Until only recently, the tools to conveniently address the question of where and when serine proteases are active within complex tissues have been lacking. In order to detect spatially restricted serine protease activities in Drosophila embryos and ovaries we introduce a technique using fluorescent synthetic and protein-based inhibitors. With this approach we have detected a novel serine protease activity with a relative mobility of 37 kDa, localized to the surface of pole cells, the germ-line precursors, in embryos between nuclear cycles 11 and 14 in development. A second novel cell-specific protease activity was localized to the tissues of early gastrulating embryos. Microinjection of inhibitors into the perivitelline space of stage 2 embryos perturbed normal embryonic development. Fluorescein-conjugated chymotrypsin inhibitor and Bowman-Birk inhibitor labeled protease activity localized to the oocyte-somatic follicle cell interface of the developing egg chamber. Our results suggest that this technique holds promise to identify new spatially restricted activities in adult Drosophila tissues and developing embryos.
55 schema:genre research_article
56 schema:inLanguage en
57 schema:isAccessibleForFree false
58 schema:isPartOf N741099d29bac40879036f86c52a6b63f
59 Nf435290c43c9483c95438c2e066e9166
60 sg:journal.1294817
61 schema:name Fluorescently labeled inhibitors detect localized serine protease activities in Drosophila melanogaster pole cells, embryos, and ovarian egg chambers
62 schema:pagination 51-60
63 schema:productId N1c5ed8a1c8234bf0a855791cee7b9f92
64 N5c793bef4cf742efa4eaffe482de121a
65 N6a46defb903e4a0b9db7d775786facac
66 N7f1ff1bd898d459ea75f87705bd9cb4d
67 Naa09b3d0bccf45eca4c1d09045b3d5c1
68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026434577
69 https://doi.org/10.1007/s00418-004-0734-5
70 schema:sdDatePublished 2019-04-11T14:26
71 schema:sdLicense https://scigraph.springernature.com/explorer/license/
72 schema:sdPublisher N05627c561f824f3f9afadcff97314920
73 schema:url http://link.springer.com/10.1007%2Fs00418-004-0734-5
74 sgo:license sg:explorer/license/
75 sgo:sdDataset articles
76 rdf:type schema:ScholarlyArticle
77 N052b4d9aff00467b81984fcc038a756a rdf:first sg:person.01016560766.59
78 rdf:rest N28bb40e517e442979ae78c4e32b16a74
79 N05627c561f824f3f9afadcff97314920 schema:name Springer Nature - SN SciGraph project
80 rdf:type schema:Organization
81 N06b7a3e75e134b1a981f0797f2e1f94e rdf:first sg:person.015305143715.14
82 rdf:rest N052b4d9aff00467b81984fcc038a756a
83 N1c5ed8a1c8234bf0a855791cee7b9f92 schema:name readcube_id
84 schema:value 7deef0bbbceccea6c3d3fc4e253f678a62e55f5e0249f5044ff2331169547b4c
85 rdf:type schema:PropertyValue
86 N28bb40e517e442979ae78c4e32b16a74 rdf:first sg:person.0652240346.27
87 rdf:rest rdf:nil
88 N2c43b3748a324d73872939da74ba8493 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Cell Nucleus
90 rdf:type schema:DefinedTerm
91 N3f090afd614e436097ad6171304039c6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Ovary
93 rdf:type schema:DefinedTerm
94 N46a37b0afc3540779bb7b3ad46139f34 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Female
96 rdf:type schema:DefinedTerm
97 N5c793bef4cf742efa4eaffe482de121a schema:name pubmed_id
98 schema:value 15609041
99 rdf:type schema:PropertyValue
100 N5eccbee1871247bbad9509a57ea2773e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Oogenesis
102 rdf:type schema:DefinedTerm
103 N6825e9ecb2614dd7830fdad0cfe1dd95 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Signal Transduction
105 rdf:type schema:DefinedTerm
106 N6a46defb903e4a0b9db7d775786facac schema:name dimensions_id
107 schema:value pub.1026434577
108 rdf:type schema:PropertyValue
109 N741099d29bac40879036f86c52a6b63f schema:issueNumber 1
110 rdf:type schema:PublicationIssue
111 N7f1ff1bd898d459ea75f87705bd9cb4d schema:name doi
112 schema:value 10.1007/s00418-004-0734-5
113 rdf:type schema:PropertyValue
114 N8432b89550a94db5894cd20072fe86ad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Cell Movement
116 rdf:type schema:DefinedTerm
117 N88cc083e495c4d20af36513b9ce9c118 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Drosophila melanogaster
119 rdf:type schema:DefinedTerm
120 N97d46cb71b8047b4aa69d8ea4c248769 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Fluorescein
122 rdf:type schema:DefinedTerm
123 N9aa6f039b30a44fa9511720efb752716 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Serine Proteinase Inhibitors
125 rdf:type schema:DefinedTerm
126 Na5780ddbc9994e7f942a416e2de23738 rdf:first sg:person.01216462212.12
127 rdf:rest N06b7a3e75e134b1a981f0797f2e1f94e
128 Na7bdba17827d4bea887b0a5c2b275894 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Embryo, Nonmammalian
130 rdf:type schema:DefinedTerm
131 Naa09b3d0bccf45eca4c1d09045b3d5c1 schema:name nlm_unique_id
132 schema:value 9506663
133 rdf:type schema:PropertyValue
134 Nc9655c86a7e54a8ca7feb9c9e8db41db schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Serine Endopeptidases
136 rdf:type schema:DefinedTerm
137 Nca7b73e8eb724bf28eb9df5e7410899c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Ovum
139 rdf:type schema:DefinedTerm
140 Nd59343801e324589ab10c7f74ff6e4bd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Fluorescent Dyes
142 rdf:type schema:DefinedTerm
143 Nd9abb9d781e349169f50b11e3c141be9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Cell Division
145 rdf:type schema:DefinedTerm
146 Ne2132253595f4431aa64693f9cbccd22 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Gastrula
148 rdf:type schema:DefinedTerm
149 Ned9e400665cb44c2961ffd9d6158049c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Animals
151 rdf:type schema:DefinedTerm
152 Nf435290c43c9483c95438c2e066e9166 schema:volumeNumber 123
153 rdf:type schema:PublicationVolume
154 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
155 schema:name Biological Sciences
156 rdf:type schema:DefinedTerm
157 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
158 schema:name Biochemistry and Cell Biology
159 rdf:type schema:DefinedTerm
160 sg:grant.2515035 http://pending.schema.org/fundedItem sg:pub.10.1007/s00418-004-0734-5
161 rdf:type schema:MonetaryGrant
162 sg:grant.2516760 http://pending.schema.org/fundedItem sg:pub.10.1007/s00418-004-0734-5
163 rdf:type schema:MonetaryGrant
164 sg:journal.1294817 schema:issn 0948-6143
165 1432-119X
166 schema:name Histochemistry and Cell Biology
167 rdf:type schema:Periodical
168 sg:person.01016560766.59 schema:affiliation https://www.grid.ac/institutes/grid.213917.f
169 schema:familyName Powers
170 schema:givenName James C.
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016560766.59
172 rdf:type schema:Person
173 sg:person.01216462212.12 schema:affiliation https://www.grid.ac/institutes/grid.5254.6
174 schema:familyName Jakobsen
175 schema:givenName Rasmus Kragh
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216462212.12
177 rdf:type schema:Person
178 sg:person.015305143715.14 schema:affiliation https://www.grid.ac/institutes/grid.267346.2
179 schema:familyName Ono
180 schema:givenName Shin
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015305143715.14
182 rdf:type schema:Person
183 sg:person.0652240346.27 schema:affiliation https://www.grid.ac/institutes/grid.5254.6
184 schema:familyName DeLotto
185 schema:givenName Robert
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652240346.27
187 rdf:type schema:Person
188 sg:pub.10.1007/978-3-662-02454-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004983365
189 https://doi.org/10.1007/978-3-662-02454-6
190 rdf:type schema:CreativeWork
191 sg:pub.10.1007/bf00291041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017141140
192 https://doi.org/10.1007/bf00291041
193 rdf:type schema:CreativeWork
194 sg:pub.10.1038/358387a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022067404
195 https://doi.org/10.1038/358387a0
196 rdf:type schema:CreativeWork
197 https://app.dimensions.ai/details/publication/pub.1004983365 schema:CreativeWork
198 https://app.dimensions.ai/details/publication/pub.1074795416 schema:CreativeWork
199 https://app.dimensions.ai/details/publication/pub.1076372774 schema:CreativeWork
200 https://app.dimensions.ai/details/publication/pub.1078471759 schema:CreativeWork
201 https://app.dimensions.ai/details/publication/pub.1081907007 schema:CreativeWork
202 https://doi.org/10.1002/047084664x.ch11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045430921
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1002/1529-0131(200109)44:9<2084::aid-art359>3.0.co;2-r schema:sameAs https://app.dimensions.ai/details/publication/pub.1041494312
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1002/jcb.240320105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015630520
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/0003-9861(61)90145-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1000992256
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/0012-1606(80)90173-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002575091
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/0012-1606(83)90025-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024139559
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/0012-1606(89)90225-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010035548
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/0092-8674(91)90381-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054549985
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/0092-8674(92)90181-b schema:sameAs https://app.dimensions.ai/details/publication/pub.1051670923
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/0092-8674(93)90393-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008112299
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/0925-4773(91)90019-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047936194
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/0962-8924(92)90246-j schema:sameAs https://app.dimensions.ai/details/publication/pub.1002097545
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/s0065-1281(96)80041-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005887409
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/s0065-2776(01)79005-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031480596
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/s0091-679x(08)60931-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037017189
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/s0889-8588(18)30282-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1076575764
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1021/bc00029a004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055153016
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1021/cr010182v schema:sameAs https://app.dimensions.ai/details/publication/pub.1009807257
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1073/pnas.081026598 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022195361
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1093/emboj/20.10.2387 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025169740
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1093/emboj/cdg224 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000579518
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1126/science.287.5461.2185 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019737566
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1146/annurev.cb.05.110189.001521 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031452008
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1159/000468898 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077639318
249 rdf:type schema:CreativeWork
250 https://www.grid.ac/institutes/grid.213917.f schema:alternateName Georgia Institute of Technology
251 schema:name Department of Chemistry and Biochemistry, Georgia Institute of Technology, GA 30332-0400, Atlanta, USA
252 rdf:type schema:Organization
253 https://www.grid.ac/institutes/grid.267346.2 schema:alternateName University of Toyama
254 schema:name Department of System Engineering of Materials and Life Science, Faculty of Engineering, Toyama University, Gofuku 3190, 930-8555, Toyama, Japan
255 rdf:type schema:Organization
256 https://www.grid.ac/institutes/grid.5254.6 schema:alternateName University of Copenhagen
257 schema:name Department of Genetics, Institute of Molecular Biology, University of Copenhagen, Øster Farimagsgade 2A, 1353, Copenhagen K, Denmark
258 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...