Pathways and microbiome modifications related to surgery and enterocolitis in Hirschsprung disease View Full Text


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Article Info

DATE

2021-10-22

AUTHORS

Roberto Biassoni, Eddi Di Marco, Margherita Squillario, Elisabetta Ugolotti, Manuela Mosconi, Maria Grazia Faticato, Girolamo Mattioli, Stefano Avanzini, Alessio Pini Prato

ABSTRACT

BackgroundHirschsprung disease (HSCR) is a congenital anomaly of the enteric nervous system. Abnormal microbiome composition was reported in HSCR patients. In this study, we addressed and analyzed microbiome modifications with relation tosurgery and HSCR associated enterocolitis (HAEC).MethodsThe faecal microbiome of 31 HSCR patients (overall 64 samples) was analyzed. HAEC was diagnosed and classified according to a combination of Pastor’s and Elhalabi’s criteria. Stool samples were analyzed by 16S sequencing (7 out of 9 polymorphic regions). Compositional and relative abundance profiles, as well as the functional potentials of the microbial community, were analyzed with the marker gene sequencing profiles using PICRUSt.ResultsThe relative abundance of Bacteroidetes showed a severe decrease with slow recovery after surgery. Conversely, Proteobacteria transiently increased their abundance. Noteworthy, a strong linkage has been found between Proteobacteria descendants and HAEC occurrences. The inferred functional analysis indicated that virulence factors and fimbriae or pili might be associated with HAEC.ConclusionsOur study, addressing microbiome dynamics, demonstrated relevant changes after surgical manipulation. Alpha-diversity analyses indicated that surgery deeply affects microbiome composition. Proteobacteria and Enterobacteriaceae seem to play a pivotal role in HAEC occurrences. Several virulence factors, such as fimbriae or pili, might explain the HAEC-predisposing potential of selected microbiomes. These results suggest some innovative therapeutic approaches that deserve to be tested in appropriate clinical trials. More... »

PAGES

1-16

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    URI

    http://scigraph.springernature.com/pub.10.1007/s00383-021-05012-y

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    DIMENSIONS

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    PUBMED

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    32 Enterobacteriaceae
    33 HAEC
    34 HSCR
    35 HSCR patients
    36 Hirschsprung's disease
    37 MethodsThe faecal microbiome
    38 Noteworthy
    39 PICRUSt
    40 Proteobacteria
    41 Proteobacteria descendants
    42 abundance
    43 abundance profiles
    44 analysis
    45 anomalies
    46 approach
    47 appropriate clinical trials
    48 changes
    49 clinical trials
    50 combination
    51 community
    52 composition
    53 congenital anomalies
    54 criteria
    55 decrease
    56 descendants
    57 disease
    58 dynamics
    59 enteric nervous system
    60 enterocolitis
    61 factors
    62 fecal microbiome
    63 fimbriae
    64 functional analysis
    65 functional potential
    66 gene sequencing profiles
    67 inferred functional analysis
    68 innovative therapeutic approaches
    69 linkage
    70 manipulation
    71 marker gene sequencing profiles
    72 microbial communities
    73 microbiome
    74 microbiome composition
    75 microbiome dynamics
    76 microbiome modification
    77 modification
    78 nervous system
    79 pastors
    80 pathway
    81 patients
    82 pili
    83 pivotal role
    84 potential
    85 profile
    86 recovery
    87 relation tosurgery
    88 relative abundance
    89 relative abundance profiles
    90 relevant changes
    91 results
    92 role
    93 samples
    94 sequencing
    95 sequencing profiles
    96 severe decrease
    97 slow recovery
    98 stool samples
    99 strong linkage
    100 study
    101 surgery
    102 surgical manipulation
    103 system
    104 therapeutic approaches
    105 tosurgery
    106 trials
    107 virulence factors
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