Embryo-specific Proteins in Cyclamen persicum Analyzed with 2-D DIGE View Full Text


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Article Info

DATE

2008-09-14

AUTHORS

Ragnhild Lyngved, Jenny Renaut, Jean-François Hausman, Tor-Henning Iversen, Anne Kathrine Hvoslef-Eide

ABSTRACT

Somatic embryogenesis can be used to produce artificial seeds of Cyclamen persicum, one of the most important ornamental plants for the European market, both as a potted plant in northern Europe and a bedding plant in the cool winters in southern Europe. The aim of this study was to obtain new insights into the molecular biology of somatic embryogenesis, which in turn can be useful for the improvement of tissue culture methodology. Total proteins were characterized from two isogenic cell lines of Cyclamen persicum, one that was embryogenic and one that never has shown any embryogenic capacity. The extracted proteins were separated by two-dimensional differential gel electrophoresis (2-D DIGE) and selected proteins were treated using the ETTAN Dalt Spot Handling Workstation. Protein identification was performed using MALDI-TOF-MS. More than 1200 Cyclamen proteins were detected; 943 proteins were common to both lines. The different protein patterns of the embryogenic and non-embryogenic cell lines were obvious: One hundred eight proteins were more abundant in the embryogenic cells, and 97 proteins in the non-embryogenic cells. Among the differentially expressed proteins, 128 were identified. MALDI-TOF-MS analysis enabled 27 spots to be proposed as candidates for embryo-specific proteins, as they were unique to the embryogenic cell line. The proteins identified are involved in a variety of cellular processes, including cell proliferation, protein processing, signal transduction, stress response, metabolism, and energy state, but the majority are involved in protein processing and metabolism. The main functions of the putative embryo-specific proteins have been discussed in proportion to their role in the somatic embryogenesis process. More... »

PAGES

353

References to SciGraph publications

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  • 2007-05-04. The continuing conundrum of the LEA proteins in THE SCIENCE OF NATURE
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  • 1998-03. Flow cytometric analyses in embryogenic and non-embryogenic callus lines of Cyclamen persicum Mill.: relation between ploidy level and competence for somatic embryogenesis in PLANT CELL REPORTS
  • 2001. The Hsp90 family of proteins in Arabidopsis thaliana in CELL STRESS AND CHAPERONES
  • 2006-02-22. Proteomic analyses of somatic and zygotic embryos of Cyclamen persicum Mill. reveal new insights into seed and germination physiology in PLANTA
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    http://scigraph.springernature.com/pub.10.1007/s00344-008-9061-8

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    28 schema:description Somatic embryogenesis can be used to produce artificial seeds of Cyclamen persicum, one of the most important ornamental plants for the European market, both as a potted plant in northern Europe and a bedding plant in the cool winters in southern Europe. The aim of this study was to obtain new insights into the molecular biology of somatic embryogenesis, which in turn can be useful for the improvement of tissue culture methodology. Total proteins were characterized from two isogenic cell lines of Cyclamen persicum, one that was embryogenic and one that never has shown any embryogenic capacity. The extracted proteins were separated by two-dimensional differential gel electrophoresis (2-D DIGE) and selected proteins were treated using the ETTAN Dalt Spot Handling Workstation. Protein identification was performed using MALDI-TOF-MS. More than 1200 Cyclamen proteins were detected; 943 proteins were common to both lines. The different protein patterns of the embryogenic and non-embryogenic cell lines were obvious: One hundred eight proteins were more abundant in the embryogenic cells, and 97 proteins in the non-embryogenic cells. Among the differentially expressed proteins, 128 were identified. MALDI-TOF-MS analysis enabled 27 spots to be proposed as candidates for embryo-specific proteins, as they were unique to the embryogenic cell line. The proteins identified are involved in a variety of cellular processes, including cell proliferation, protein processing, signal transduction, stress response, metabolism, and energy state, but the majority are involved in protein processing and metabolism. The main functions of the putative embryo-specific proteins have been discussed in proportion to their role in the somatic embryogenesis process.
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    36 DIGE
    37 Europe
    38 European market
    39 MALDI-TOF
    40 MS
    41 MS analysis
    42 aim
    43 analysis
    44 artificial seeds
    45 biology
    46 candidates
    47 capacity
    48 cell lines
    49 cell proliferation
    50 cells
    51 cellular processes
    52 cool winters
    53 culture methodology
    54 different protein patterns
    55 differential gel electrophoresis
    56 electrophoresis
    57 embryo-specific proteins
    58 embryogenesis
    59 embryogenesis process
    60 embryogenic capacity
    61 embryogenic cell lines
    62 embryogenic cells
    63 energy states
    64 function
    65 gel electrophoresis
    66 identification
    67 important ornamental plant
    68 improvement
    69 insights
    70 isogenic cell lines
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    72 main function
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    75 metabolism
    76 methodology
    77 molecular biology
    78 new insights
    79 non-embryogenic cell lines
    80 non-embryogenic cells
    81 northern Europe
    82 one
    83 ornamental plants
    84 patterns
    85 persicum
    86 plants
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    88 process
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    90 proliferation
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    92 protein
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    102 southern Europe
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    115 schema:name Embryo-specific Proteins in Cyclamen persicum Analyzed with 2-D DIGE
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