Ontology type: schema:ScholarlyArticle
2008-09-14
AUTHORSRagnhild Lyngved, Jenny Renaut, Jean-François Hausman, Tor-Henning Iversen, Anne Kathrine Hvoslef-Eide
ABSTRACTSomatic embryogenesis can be used to produce artificial seeds of Cyclamen persicum, one of the most important ornamental plants for the European market, both as a potted plant in northern Europe and a bedding plant in the cool winters in southern Europe. The aim of this study was to obtain new insights into the molecular biology of somatic embryogenesis, which in turn can be useful for the improvement of tissue culture methodology. Total proteins were characterized from two isogenic cell lines of Cyclamen persicum, one that was embryogenic and one that never has shown any embryogenic capacity. The extracted proteins were separated by two-dimensional differential gel electrophoresis (2-D DIGE) and selected proteins were treated using the ETTAN Dalt Spot Handling Workstation. Protein identification was performed using MALDI-TOF-MS. More than 1200 Cyclamen proteins were detected; 943 proteins were common to both lines. The different protein patterns of the embryogenic and non-embryogenic cell lines were obvious: One hundred eight proteins were more abundant in the embryogenic cells, and 97 proteins in the non-embryogenic cells. Among the differentially expressed proteins, 128 were identified. MALDI-TOF-MS analysis enabled 27 spots to be proposed as candidates for embryo-specific proteins, as they were unique to the embryogenic cell line. The proteins identified are involved in a variety of cellular processes, including cell proliferation, protein processing, signal transduction, stress response, metabolism, and energy state, but the majority are involved in protein processing and metabolism. The main functions of the putative embryo-specific proteins have been discussed in proportion to their role in the somatic embryogenesis process. More... »
PAGES353
http://scigraph.springernature.com/pub.10.1007/s00344-008-9061-8
DOIhttp://dx.doi.org/10.1007/s00344-008-9061-8
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1038423393
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "S\u00f8r-Tr\u00f8ndelag University College, Rotvoll All\u00e9 1, 7004, Trondheim, Norway",
"id": "http://www.grid.ac/institutes/grid.5947.f",
"name": [
"The Plant Biocentre, Department of Biology, Norwegian University of Science and Technology, 7491, Trondheim, Norway",
"S\u00f8r-Tr\u00f8ndelag University College, Rotvoll All\u00e9 1, 7004, Trondheim, Norway"
],
"type": "Organization"
},
"familyName": "Lyngved",
"givenName": "Ragnhild",
"id": "sg:person.013724442417.82",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013724442417.82"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Environment and Agrobiotechnologies, Centre de Recherche Public - Gabriel Lippmann, 41, rue du Brill, 4422, Belvaux, Luxembourg",
"id": "http://www.grid.ac/institutes/grid.423669.c",
"name": [
"Department of Environment and Agrobiotechnologies, Centre de Recherche Public - Gabriel Lippmann, 41, rue du Brill, 4422, Belvaux, Luxembourg"
],
"type": "Organization"
},
"familyName": "Renaut",
"givenName": "Jenny",
"id": "sg:person.01176445346.39",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01176445346.39"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Environment and Agrobiotechnologies, Centre de Recherche Public - Gabriel Lippmann, 41, rue du Brill, 4422, Belvaux, Luxembourg",
"id": "http://www.grid.ac/institutes/grid.423669.c",
"name": [
"Department of Environment and Agrobiotechnologies, Centre de Recherche Public - Gabriel Lippmann, 41, rue du Brill, 4422, Belvaux, Luxembourg"
],
"type": "Organization"
},
"familyName": "Hausman",
"givenName": "Jean-Fran\u00e7ois",
"id": "sg:person.01167415303.51",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167415303.51"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "The Plant Biocentre, Department of Biology, Norwegian University of Science and Technology, 7491, Trondheim, Norway",
"id": "http://www.grid.ac/institutes/grid.5947.f",
"name": [
"The Plant Biocentre, Department of Biology, Norwegian University of Science and Technology, 7491, Trondheim, Norway"
],
"type": "Organization"
},
"familyName": "Iversen",
"givenName": "Tor-Henning",
"id": "sg:person.0776624464.73",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776624464.73"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "The Plant Cell Laboratory, Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, P.O. Box 5003, 1432, Aas, Norway",
"id": "http://www.grid.ac/institutes/grid.19477.3c",
"name": [
"The Plant Cell Laboratory, Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, P.O. Box 5003, 1432, Aas, Norway"
],
"type": "Organization"
},
"familyName": "Hvoslef-Eide",
"givenName": "Anne Kathrine",
"id": "sg:person.0670172072.91",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0670172072.91"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/978-94-011-0485-2_8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007313574",
"https://doi.org/10.1007/978-94-011-0485-2_8"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-94-011-0307-7_50",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020540666",
"https://doi.org/10.1007/978-94-011-0307-7_50"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00299-007-0327-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048579337",
"https://doi.org/10.1007/s00299-007-0327-6"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11103-007-9234-z",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020193870",
"https://doi.org/10.1007/s11103-007-9234-z"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00425-006-0238-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006596170",
"https://doi.org/10.1007/s00425-006-0238-8"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/a:1017914312743",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005223667",
"https://doi.org/10.1023/a:1017914312743"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00023415",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006476418",
"https://doi.org/10.1007/bf00023415"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/a:1015673200621",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007251281",
"https://doi.org/10.1023/a:1015673200621"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00114-007-0254-y",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043445605",
"https://doi.org/10.1007/s00114-007-0254-y"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11240-004-6662-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003923167",
"https://doi.org/10.1007/s11240-004-6662-1"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1079/ivp2006806",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037716373",
"https://doi.org/10.1079/ivp2006806"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00016072",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000334870",
"https://doi.org/10.1007/bf00016072"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00299-007-0438-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016150633",
"https://doi.org/10.1007/s00299-007-0438-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.2478/s11658-006-0044-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004963320",
"https://doi.org/10.2478/s11658-006-0044-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00299-006-0130-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027292240",
"https://doi.org/10.1007/s00299-006-0130-9"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00232131",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009732222",
"https://doi.org/10.1007/bf00232131"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11240-006-9139-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002503181",
"https://doi.org/10.1007/s11240-006-9139-6"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00195672",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004029151",
"https://doi.org/10.1007/bf00195672"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1379/1466-1268(2001)006<0238:thfopi>2.0.co;2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046386308",
"https://doi.org/10.1379/1466-1268(2001)006<0238:thfopi>2.0.co;2"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s002990050414",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012818750",
"https://doi.org/10.1007/s002990050414"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00344-005-0033-y",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029522363",
"https://doi.org/10.1007/s00344-005-0033-y"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/a:1024033216561",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018150911",
"https://doi.org/10.1023/a:1024033216561"
],
"type": "CreativeWork"
}
],
"datePublished": "2008-09-14",
"datePublishedReg": "2008-09-14",
"description": "Somatic embryogenesis can be used to produce artificial seeds of Cyclamen persicum, one of the most important ornamental plants for the European market, both as a potted plant in northern Europe and a bedding plant in the cool winters in southern Europe. The aim of this study was to obtain new insights into the molecular biology of somatic embryogenesis, which in turn can be useful for the improvement of tissue culture methodology. Total proteins were characterized from two isogenic cell lines of Cyclamen persicum, one that was embryogenic and one that never has shown any embryogenic capacity. The extracted proteins were separated by two-dimensional differential gel electrophoresis (2-D DIGE) and selected proteins were treated using the ETTAN Dalt Spot Handling Workstation. Protein identification was performed using MALDI-TOF-MS. More than 1200 Cyclamen proteins were detected; 943 proteins were common to both lines. The different protein patterns of the embryogenic and non-embryogenic cell lines were obvious: One hundred eight proteins were more abundant in the embryogenic cells, and 97 proteins in the non-embryogenic cells. Among the differentially expressed proteins, 128 were identified. MALDI-TOF-MS analysis enabled 27 spots to be proposed as candidates for embryo-specific proteins, as they were unique to the embryogenic cell line. The proteins identified are involved in a variety of cellular processes, including cell proliferation, protein processing, signal transduction, stress response, metabolism, and energy state, but the majority are involved in protein processing and metabolism. The main functions of the putative embryo-specific proteins have been discussed in proportion to their role in the somatic embryogenesis process.",
"genre": "article",
"id": "sg:pub.10.1007/s00344-008-9061-8",
"inLanguage": "en",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1124807",
"issn": [
"0721-7595",
"1435-8107"
],
"name": "Journal of Plant Growth Regulation",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "4",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "27"
}
],
"keywords": [
"embryo-specific proteins",
"Cyclamen persicum",
"somatic embryogenesis",
"protein processing",
"two-dimensional differential gel electrophoresis",
"cell lines",
"non-embryogenic cell lines",
"important ornamental plant",
"non-embryogenic cells",
"somatic embryogenesis process",
"isogenic cell lines",
"embryogenic cell lines",
"differential gel electrophoresis",
"MALDI-TOF",
"different protein patterns",
"cellular processes",
"signal transduction",
"artificial seeds",
"embryogenic cells",
"embryogenesis process",
"ornamental plants",
"potted plants",
"protein identification",
"stress response",
"molecular biology",
"protein patterns",
"embryogenic capacity",
"tissue culture methodology",
"protein",
"plants",
"cell proliferation",
"cool winters",
"embryogenesis",
"gel electrophoresis",
"persicum",
"new insights",
"northern Europe",
"MS analysis",
"culture methodology",
"metabolism",
"cells",
"southern Europe",
"transduction",
"DIGE",
"biology",
"total protein",
"main function",
"lines",
"seeds",
"proliferation",
"electrophoresis",
"identification",
"insights",
"winter",
"role",
"Europe",
"patterns",
"function",
"spots",
"variety",
"response",
"process",
"MS",
"turn",
"candidates",
"proportion",
"energy states",
"analysis",
"majority",
"capacity",
"processing",
"study",
"state",
"one",
"aim",
"methodology",
"improvement",
"European market",
"market",
"workstations"
],
"name": "Embryo-specific Proteins in Cyclamen persicum Analyzed with 2-D DIGE",
"pagination": "353",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1038423393"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s00344-008-9061-8"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s00344-008-9061-8",
"https://app.dimensions.ai/details/publication/pub.1038423393"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-10T09:56",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220509/entities/gbq_results/article/article_459.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s00344-008-9061-8"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00344-008-9061-8'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00344-008-9061-8'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00344-008-9061-8'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00344-008-9061-8'
This table displays all metadata directly associated to this object as RDF triples.
262 TRIPLES
22 PREDICATES
127 URIs
97 LITERALS
6 BLANK NODES