Transgenic grapevine plants expressing a rice chitinase with enhanced resistance to fungal pathogens View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-06

AUTHORS

T. Yamamoto, H. Iketani, H. Ieki, Y. Nishizawa, K. Notsuka, T. Hibi, T. Hayashi, N. Matsuta

ABSTRACT

The rice chitinase gene (RCC2), classified as class I chitinase, was introduced into the somatic embryos of grapevine (Vitis vinifera L. cv. Neo Muscut) by Agrobacterium infection. After co-cultivation with Agrobacterium, somatic embryos were transferred onto Murashige and Skoog hormone-free medium supplemented with 50 mg/l kanamycin. Transformed secondary or tertiary embryos were selected, and then more than 20 transgenic plantlets were recovered. Two transformants showed enhanced resistance against powdery mildew caused by Uncinula necator. Few disease symptoms were observed on leaves of these transformants compared with those of the non-transformant, although browning and necrotic symptoms, which seemed to constitute a hypersensitive reaction, were observed. Scanning electron microscopic observation revealed that conidial germination, mycelial growth and conidial formation were suppressed on the leaf surface of the transformant. The transgenic grapevines obtained also exhibited slight resistance against Elisinoe ampelina inducing anthracnose, resulting in a reduction in disease lesions. The relationship between the expression of the foreign chitinase gene and the disease resistance is discussed. More... »

PAGES

639-646

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002999900174

DOI

http://dx.doi.org/10.1007/s002999900174

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029266056

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30754799


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