Transgenic yellow lupin (Lupinus luteus) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-05

AUTHORS

H. Li, S. J. Wylie, M. G. K. Jones

ABSTRACT

Transgenic yellow lupin (Lupinus luteus L.) plants have been generated by meristem co-cultivation with Agrobacterium tumefaciens. The binary plasmid pPZBNIa contains the bar gene under the control of a CaMV 35 S promoter. The transformation method involves inoculation of embryonic axis explants with A. tumefaciens, flooding the meristem with glufosinate, and initial culture on non-selective medium. Shoots were transferred to culture medium containing 20 mg/l glufosinate. Following subculture, shoots were grafted onto non-transgenic narrow-leafed lupin (L. angustifolius L.) seedling rootstocks, or rooted in vitro. The overall transformation efficiency, as determined at the T1 generation, was 0.05%-0.75%. The transgenic nature of plants grown to the T6 generation was confirmed by phosphinothricin acetyl transferase, PCR and Southern analyses. More... »

PAGES

634-637

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002990050785

DOI

http://dx.doi.org/10.1007/s002990050785

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029692302

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30754829


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