Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-04

AUTHORS

P. A. Donaldson, D. H. Simmonds

ABSTRACT

Short-season adapted soybean [Glycine max (L.) Merrill] genotypes (maturity group 0 and 00) were susceptible to Agrobacterium tumefaciens in tumor-formation assays with A. tumefaciens strains A281, C58 and ACH5. The response was bacterial-strain and plant-cultivar dependent. In vitro Agrobacterium-mediated transformation of cotyledonary node explants of these genotypes with A. tumefaciens EHA105/pBI121 was inefficient but resulted in a transgenic AC Colibri plant carrying a linked insertion of the neomycin phosphotransferase and β-glucuronidase (gus) transgenes. The transgenes were transmitted to the progeny and stable gus expression was detected in the T7 generation. The low rate of recovery of transgenic plants from the co-cultured cotyledonary explants was attributed to inefficient transformation of regenerable cells, and/or poor selection or survival of such cells and not to poor susceptibility to Agrobacterium, since, depending on the cultivar, explants were transformed at a rate of 27–92%, but transformation events were usually restricted to non-regenerable callus. More... »

PAGES

478-484

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002990050759

DOI

http://dx.doi.org/10.1007/s002990050759

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029499433

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30754886


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Plant Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Building 21, Central Experimental Farm, Ottawa, Ontario, Canada K1A OC6 Fax: +1\u2013613\u20137596566 e-mail: simmondsdh@em.agr.ca, CA", 
          "id": "http://www.grid.ac/institutes/grid.55614.33", 
          "name": [
            "Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Building 21, Central Experimental Farm, Ottawa, Ontario, Canada K1A OC6 Fax: +1\u2013613\u20137596566 e-mail: simmondsdh@em.agr.ca, CA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Donaldson", 
        "givenName": "P. A.", 
        "id": "sg:person.010204457135.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010204457135.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Building 21, Central Experimental Farm, Ottawa, Ontario, Canada K1A OC6 Fax: +1\u2013613\u20137596566 e-mail: simmondsdh@em.agr.ca, CA", 
          "id": "http://www.grid.ac/institutes/grid.55614.33", 
          "name": [
            "Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Building 21, Central Experimental Farm, Ottawa, Ontario, Canada K1A OC6 Fax: +1\u2013613\u20137596566 e-mail: simmondsdh@em.agr.ca, CA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Simmonds", 
        "givenName": "D. H.", 
        "id": "sg:person.0740512262.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0740512262.51"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2000-04", 
    "datePublishedReg": "2000-04-01", 
    "description": "Abstract\u2002Short-season adapted soybean [Glycine max (L.) Merrill] genotypes (maturity group 0 and 00) were susceptible to Agrobacterium tumefaciens in tumor-formation assays with A. tumefaciens strains A281, C58 and ACH5. The response was bacterial-strain and plant-cultivar dependent. In vitro Agrobacterium-mediated transformation of cotyledonary node explants of these genotypes with A. tumefaciens EHA105/pBI121 was inefficient but resulted in a transgenic AC Colibri plant carrying a linked insertion of the neomycin phosphotransferase and \u03b2-glucuronidase (gus) transgenes. The transgenes were transmitted to the progeny and stable gus expression was detected in the T7 generation. The low rate of recovery of transgenic plants from the co-cultured cotyledonary explants was attributed to inefficient transformation of regenerable cells, and/or poor selection or survival of such cells and not to poor susceptibility to Agrobacterium, since, depending on the cultivar, explants were transformed at a rate of 27\u201392%, but transformation events were usually restricted to non-regenerable callus.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s002990050759", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1124809", 
        "issn": [
          "0721-7714", 
          "1432-203X"
        ], 
        "name": "Plant Cell Reports", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "19"
      }
    ], 
    "keywords": [
      "tumor formation assays", 
      "poor susceptibility", 
      "lower rates", 
      "such cells", 
      "cells", 
      "explants", 
      "susceptibility", 
      "genotypes", 
      "survival", 
      "non-regenerable callus", 
      "\u03b2-glucuronidase (GUS) transgene", 
      "inefficient transformation", 
      "transgene", 
      "cotyledonary node explants", 
      "transgenic plants", 
      "short-season soybean", 
      "rate", 
      "T7 generation", 
      "assays", 
      "regenerable cells", 
      "Agrobacterium tumefaciens", 
      "neomycin phosphotransferase", 
      "A. tumefaciens", 
      "gus expression", 
      "expression", 
      "transformation events", 
      "node explants", 
      "tumefaciens", 
      "cotyledonary explants", 
      "response", 
      "poor selection", 
      "soybean genotypes", 
      "node transformation", 
      "Agrobacterium", 
      "recovery", 
      "insertion", 
      "events", 
      "plants", 
      "pBI121", 
      "phosphotransferase", 
      "Ach5", 
      "C58", 
      "A281", 
      "callus", 
      "cultivars", 
      "soybean", 
      "selection", 
      "generation", 
      "transformation"
    ], 
    "name": "Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean", 
    "pagination": "478-484", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1029499433"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s002990050759"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30754886"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s002990050759", 
      "https://app.dimensions.ai/details/publication/pub.1029499433"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-10-01T06:31", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_343.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s002990050759"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002990050759'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002990050759'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002990050759'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002990050759'


 

This table displays all metadata directly associated to this object as RDF triples.

117 TRIPLES      20 PREDICATES      75 URIs      67 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s002990050759 schema:about anzsrc-for:06
2 anzsrc-for:0607
3 schema:author N1abb5190f63c4431aa2cfd0e76bd9247
4 schema:datePublished 2000-04
5 schema:datePublishedReg 2000-04-01
6 schema:description Abstract Short-season adapted soybean [Glycine max (L.) Merrill] genotypes (maturity group 0 and 00) were susceptible to Agrobacterium tumefaciens in tumor-formation assays with A. tumefaciens strains A281, C58 and ACH5. The response was bacterial-strain and plant-cultivar dependent. In vitro Agrobacterium-mediated transformation of cotyledonary node explants of these genotypes with A. tumefaciens EHA105/pBI121 was inefficient but resulted in a transgenic AC Colibri plant carrying a linked insertion of the neomycin phosphotransferase and β-glucuronidase (gus) transgenes. The transgenes were transmitted to the progeny and stable gus expression was detected in the T7 generation. The low rate of recovery of transgenic plants from the co-cultured cotyledonary explants was attributed to inefficient transformation of regenerable cells, and/or poor selection or survival of such cells and not to poor susceptibility to Agrobacterium, since, depending on the cultivar, explants were transformed at a rate of 27–92%, but transformation events were usually restricted to non-regenerable callus.
7 schema:genre article
8 schema:isAccessibleForFree false
9 schema:isPartOf N243855dc8c7e406a9cfc28501d5a7309
10 N45e550a4c24e4d3d987814fef8f75afe
11 sg:journal.1124809
12 schema:keywords A. tumefaciens
13 A281
14 Ach5
15 Agrobacterium
16 Agrobacterium tumefaciens
17 C58
18 T7 generation
19 assays
20 callus
21 cells
22 cotyledonary explants
23 cotyledonary node explants
24 cultivars
25 events
26 explants
27 expression
28 generation
29 genotypes
30 gus expression
31 inefficient transformation
32 insertion
33 lower rates
34 neomycin phosphotransferase
35 node explants
36 node transformation
37 non-regenerable callus
38 pBI121
39 phosphotransferase
40 plants
41 poor selection
42 poor susceptibility
43 rate
44 recovery
45 regenerable cells
46 response
47 selection
48 short-season soybean
49 soybean
50 soybean genotypes
51 such cells
52 survival
53 susceptibility
54 transformation
55 transformation events
56 transgene
57 transgenic plants
58 tumefaciens
59 tumor formation assays
60 β-glucuronidase (GUS) transgene
61 schema:name Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean
62 schema:pagination 478-484
63 schema:productId N2f7e4f37ae8e47638ba0c65e9b9c0c63
64 N3048004a5fd140b39e06008e008a431a
65 Nfa65dee79b6945fea1a6ecd68ac6e1a3
66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029499433
67 https://doi.org/10.1007/s002990050759
68 schema:sdDatePublished 2022-10-01T06:31
69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
70 schema:sdPublisher N331b0fb50cc7423a9c500d47ebcb4478
71 schema:url https://doi.org/10.1007/s002990050759
72 sgo:license sg:explorer/license/
73 sgo:sdDataset articles
74 rdf:type schema:ScholarlyArticle
75 N1abb5190f63c4431aa2cfd0e76bd9247 rdf:first sg:person.010204457135.74
76 rdf:rest N1ca903c92ff34ad78a4ff1ff6ebff04a
77 N1ca903c92ff34ad78a4ff1ff6ebff04a rdf:first sg:person.0740512262.51
78 rdf:rest rdf:nil
79 N243855dc8c7e406a9cfc28501d5a7309 schema:issueNumber 5
80 rdf:type schema:PublicationIssue
81 N2f7e4f37ae8e47638ba0c65e9b9c0c63 schema:name doi
82 schema:value 10.1007/s002990050759
83 rdf:type schema:PropertyValue
84 N3048004a5fd140b39e06008e008a431a schema:name dimensions_id
85 schema:value pub.1029499433
86 rdf:type schema:PropertyValue
87 N331b0fb50cc7423a9c500d47ebcb4478 schema:name Springer Nature - SN SciGraph project
88 rdf:type schema:Organization
89 N45e550a4c24e4d3d987814fef8f75afe schema:volumeNumber 19
90 rdf:type schema:PublicationVolume
91 Nfa65dee79b6945fea1a6ecd68ac6e1a3 schema:name pubmed_id
92 schema:value 30754886
93 rdf:type schema:PropertyValue
94 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
95 schema:name Biological Sciences
96 rdf:type schema:DefinedTerm
97 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
98 schema:name Plant Biology
99 rdf:type schema:DefinedTerm
100 sg:journal.1124809 schema:issn 0721-7714
101 1432-203X
102 schema:name Plant Cell Reports
103 schema:publisher Springer Nature
104 rdf:type schema:Periodical
105 sg:person.010204457135.74 schema:affiliation grid-institutes:grid.55614.33
106 schema:familyName Donaldson
107 schema:givenName P. A.
108 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010204457135.74
109 rdf:type schema:Person
110 sg:person.0740512262.51 schema:affiliation grid-institutes:grid.55614.33
111 schema:familyName Simmonds
112 schema:givenName D. H.
113 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0740512262.51
114 rdf:type schema:Person
115 grid-institutes:grid.55614.33 schema:alternateName Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Building 21, Central Experimental Farm, Ottawa, Ontario, Canada K1A OC6 Fax: +1–613–7596566 e-mail: simmondsdh@em.agr.ca, CA
116 schema:name Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Building 21, Central Experimental Farm, Ottawa, Ontario, Canada K1A OC6 Fax: +1–613–7596566 e-mail: simmondsdh@em.agr.ca, CA
117 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...