Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-04

AUTHORS

P. A. Donaldson, D. H. Simmonds

ABSTRACT

Short-season adapted soybean [Glycine max (L.) Merrill] genotypes (maturity group 0 and 00) were susceptible to Agrobacterium tumefaciens in tumor-formation assays with A. tumefaciens strains A281, C58 and ACH5. The response was bacterial-strain and plant-cultivar dependent. In vitro Agrobacterium-mediated transformation of cotyledonary node explants of these genotypes with A. tumefaciens EHA105/pBI121 was inefficient but resulted in a transgenic AC Colibri plant carrying a linked insertion of the neomycin phosphotransferase and β-glucuronidase (gus) transgenes. The transgenes were transmitted to the progeny and stable gus expression was detected in the T7 generation. The low rate of recovery of transgenic plants from the co-cultured cotyledonary explants was attributed to inefficient transformation of regenerable cells, and/or poor selection or survival of such cells and not to poor susceptibility to Agrobacterium, since, depending on the cultivar, explants were transformed at a rate of 27–92%, but transformation events were usually restricted to non-regenerable callus. More... »

PAGES

478-484

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002990050759

DOI

http://dx.doi.org/10.1007/s002990050759

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029499433

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30754886


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