Electrotransformation of Chlorella vulgaris View Full Text


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Article Info

DATE

1999-05

AUTHORS

K.-C. Chow, W. L. Tung

ABSTRACT

Using hygromycin B resistance as a marker for selection, we have established the conditions required for the transformation of Chlorella vulgaris. The exponentially grown C. vulgaris cells were transformed by electroporation with plasmid pIG121-Hm, and transformants were selected with hygromycin B at a concentration of 50 μg/ml. Cell extracts prepared from the late-log cultures of the transformants exhibited glucuronidase activities as conferred by the gus gene on pIG121-Hm. The maintenance of plasmid in the algal cells seemed to be transient as many cultures derived from the hygromycin B-resistant colonies gradually lost the hygromycin resistance upon prolonged growth. The result of Southern blotting of the genomic DNAs prepared from transformant cultures exhibiting persistent hygromycin resistance showed that integration of part of the plasmid DNA into the host chromosome had taken place. More... »

PAGES

778-780

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002990050660

DOI

http://dx.doi.org/10.1007/s002990050660

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006700866


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