Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-12

AUTHORS

M. Cervera, J. A. Pina, J. Juárez, L. Navarro, L. Peña

ABSTRACT

The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of citrange (Citrus sinensis L. Osbeck×Poncirus trifoliata L. Raf.) have been investigated. Factors such as cocultivation period, preculture of explants, use of acetosyringone or feeder plates during cocultivation, cocultivation on a medium rich in auxins, postcultivation in darkness, and different kanamycin concentrations for selection were assessed. A 3-day cocultivation on a medium rich in auxins improved transformation frequencies, since it increased the number of dividing cells competent for transformation, at the cut ends of the explants. Exposure of explants to darkness for 4 weeks on selection medium resulted in further callus development and increased the regeneration frequency of transgenic shoots. Furthermore, this treatment drastically reduced the number of regenerated escape shoots. A transformation efficiency of 41.3% was achieved using the optimized transformation procedure. More... »

PAGES

271-278

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002990050570

DOI

http://dx.doi.org/10.1007/s002990050570

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007201059

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30744234


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