Plant regeneration from protoplasts isolated from friable embryogenic callus of cassava View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-11

AUTHORS

E. Sofiari, C. J. J. M. Raemakers, J. E. M. Bergervoet, E. Jacobsen, R. G. F. Visser

ABSTRACT

Protoplasts were isolated from friable embryogenic callus (FEC) and from suspensions derived from FEC of cassava genotype TMS60444. Suspensions yielded the highest number of protoplasts (1.5×106 protoplasts/g fresh weight). Protoplasts plated at a density of 105–106/ml in a medium supplemented with 0.5 mg/l α-naphthaleneacetic acid and 1 mg/l zeatin began dividing after 3 days, and after 30 days this resulted in an absolute plating efficiency as high as 2.5%. After 2 months of culture, 60% of the developed calli were highly friable and in appearance identical to the original FEC. The protoplast derived FEC was first purified through two rounds of selection of 3 weeks each before beeing cultured for regeneration of plants. This was done by culturing the protoplast-derived FEC for 11 weeks on maturation medium, yielding a maximum of 184 organized embryos per 10.000 initially cultured protoplasts. Most of the organized embryos were torpedo shaped and matured after they had been isolated from the calli and transferred to fresh medium. Mature embryos were multiplied by secondary somatic embryogenesis at high efficiency (>90%) on a medium supplemented with 8 mg/l 2,4-dichlorophenoxyacetic acid. About 30% of the mature secondary somatic embryos developed into shoots after transfer to a medium supplemented with 1 mg/l N6-benzylaminopurine (BAP). Shoots rooted readily on a medium without BAP. More... »

PAGES

159-165

Journal

TITLE

Plant Cell Reports

ISSUE

1-2

VOLUME

18

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002990050550

DOI

http://dx.doi.org/10.1007/s002990050550

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010494655


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