Micropropagation of gum karaya (Sterculia urens) by adventitious shoot formation and somatic embryogenesis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-09

AUTHORS

V. G. Sunnichan, K. R. Shivanna, H. Y. Mohan Ram

ABSTRACT

Nodal explants from selected trees of gum karaya (Sterculia urens Roxb.) in the adult growth phase cultured on Murashige and Skoog (MS) medium supplemented with 6.62 μM N6-benzylaminopurine (BAP) produced an average of six adventitious shoots in 30 days. Shoots were rooted in vitro on 1/4-strength MS medium containing 9.82 μM indole-3-butyric acid. Nodulated callus was produced from hypocotyl explants cultured on MS medium supplemented with 4.52 μM 2,4-dichlorophenoxyacetic acid and 8.90 μM BAP. Somatic embryos developed when the nodulated callus was transferred to MS medium containing 0.45 μM thidiazuron (TDZ). TDZ treatment for 2 days gave the optimum response. Over 30% of the somatic embryos developed into plantlets when transferred to 1/4-strength MS basal medium without any growth regulators. Plantlets produced from adventitious shoots and somatic embryos were acclimatized to ex vitro conditions and established in the field. More... »

PAGES

951-956

Journal

TITLE

Plant Cell Reports

ISSUE

12

VOLUME

17

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002990050516

DOI

http://dx.doi.org/10.1007/s002990050516

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009058179

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30736546


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