Regeneration of transgenic cassava plants (Manihot esculenta Crantz) through Agrobacterium-mediated transformation of embryogenic suspension cultures View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-08

AUTHORS

A. E. González, C. Schöpke, N. J. Taylor, R. N. Beachy, C. M. Fauquet

ABSTRACT

A protocol was developed for Agrobacterium-mediated transformation of embryogenic suspension cultures of cassava. The bacterial strain ABI containing the binary vector pMON977 with the nptII gene as selectable marker and an intron-interrupted uidA gene (encoding β-glucuronidase) as visible marker was used for the experiments. Selection of transformed tissue with paromomycin resulted in the establishment of antibiotic-resistant, β-glucuronidase-expressing lines of friable embryogenic callus, from which embryos and subsequently plants were regenerated. Southern blot analysis demonstrated stable integration of the uidA gene into the cassava genome in five lines of transformed embryogenic suspension cultures and in two plant lines. More... »

PAGES

827-831

Journal

TITLE

Plant Cell Reports

ISSUE

11

VOLUME

17

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002990050492

DOI

http://dx.doi.org/10.1007/s002990050492

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043692914

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30736551


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