Co-transformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-04

AUTHORS

M. Daley, V. C. Knauf, K. R. Summerfelt, J. C. Turner

ABSTRACT

Co-transformation was investigated as a method that would allow the use of a selectable marker during plant regeneration followed by recovery of progeny which contain the desired gene(s) but lack a marker gene. Rapeseed (Brassica napus cv `212/86') and tobacco (Nicotiana tabacum cv `Xanthi NC') were co-cultivated with a single Agrobacterium tumefaciens strain containing two binary plasmids. Genes from both plasmids were expressed in approximately 50% of the primary transformants. Progeny expressing only one of the transgenes were observed in about 50% of the co-transformed lines, indicating that the genes were inserted at different loci. This single-strain co-transformation method allowed the use of a selectable marker during plant regeneration and subsequent recovery of marker-free progeny. More... »

PAGES

489-496

Journal

TITLE

Plant Cell Reports

ISSUE

6-7

VOLUME

17

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002990050430

DOI

http://dx.doi.org/10.1007/s002990050430

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009947215

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30736624


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Monsanto (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418554.9", 
          "name": [
            "Calgene Inc., 1920 Fifth St., Davis, CA 95616, USA, US"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Daley", 
        "givenName": "M.", 
        "id": "sg:person.0710075556.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0710075556.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Monsanto (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418554.9", 
          "name": [
            "Calgene Inc., 1920 Fifth St., Davis, CA 95616, USA, US"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Knauf", 
        "givenName": "V. C.", 
        "id": "sg:person.01051134614.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051134614.91"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Monsanto (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418554.9", 
          "name": [
            "Calgene Inc., 1920 Fifth St., Davis, CA 95616, USA, US"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Summerfelt", 
        "givenName": "K. R.", 
        "id": "sg:person.01247363731.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247363731.84"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Monsanto (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418554.9", 
          "name": [
            "Calgene Inc., 1920 Fifth St., Davis, CA 95616, USA, US"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Turner", 
        "givenName": "J. C.", 
        "type": "Person"
      }
    ], 
    "datePublished": "1998-04", 
    "datePublishedReg": "1998-04-01", 
    "description": "Co-transformation was investigated as a method that would allow the use of a selectable marker during plant regeneration followed by recovery of progeny which contain the desired gene(s) but lack a marker gene. Rapeseed (Brassica napus cv `212/86') and tobacco (Nicotiana tabacum cv `Xanthi NC') were co-cultivated with a single Agrobacterium tumefaciens strain containing two binary plasmids. Genes from both plasmids were expressed in approximately 50% of the primary transformants. Progeny expressing only one of the transgenes were observed in about 50% of the co-transformed lines, indicating that the genes were inserted at different loci. This single-strain co-transformation method allowed the use of a selectable marker during plant regeneration and subsequent recovery of marker-free progeny.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s002990050430", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1124809", 
        "issn": [
          "0721-7714", 
          "1432-203X"
        ], 
        "name": "Plant Cell Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6-7", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "17"
      }
    ], 
    "name": "Co-transformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants", 
    "pagination": "489-496", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "2f6345603d2b227b6a0b59590b3dc03c585841d714096c14a98f5dfbc3c7d591"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30736624"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9880970"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s002990050430"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1009947215"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s002990050430", 
      "https://app.dimensions.ai/details/publication/pub.1009947215"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:57", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000347_0000000347/records_89807_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs002990050430"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002990050430'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002990050430'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002990050430'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002990050430'


 

This table displays all metadata directly associated to this object as RDF triples.

89 TRIPLES      20 PREDICATES      29 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s002990050430 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author Ncc16f37c7bdb488da7646a9cf86fa4f5
4 schema:datePublished 1998-04
5 schema:datePublishedReg 1998-04-01
6 schema:description Co-transformation was investigated as a method that would allow the use of a selectable marker during plant regeneration followed by recovery of progeny which contain the desired gene(s) but lack a marker gene. Rapeseed (Brassica napus cv `212/86') and tobacco (Nicotiana tabacum cv `Xanthi NC') were co-cultivated with a single Agrobacterium tumefaciens strain containing two binary plasmids. Genes from both plasmids were expressed in approximately 50% of the primary transformants. Progeny expressing only one of the transgenes were observed in about 50% of the co-transformed lines, indicating that the genes were inserted at different loci. This single-strain co-transformation method allowed the use of a selectable marker during plant regeneration and subsequent recovery of marker-free progeny.
7 schema:genre research_article
8 schema:inLanguage en
9 schema:isAccessibleForFree false
10 schema:isPartOf N4c89520deaf94cfd980c8cfa20d533b7
11 N6d511bb5bbf14095bb47887cac1f11bf
12 sg:journal.1124809
13 schema:name Co-transformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants
14 schema:pagination 489-496
15 schema:productId N39aaf6169a5f4a8097313cea17a6d050
16 N3a18cc99206a4ea9a5ad0037ec3cf67e
17 N8fd1a4ab6a034353b70cffeebced6bde
18 Nd904daad24cc4887b7eaa9bd01143e9e
19 Ne3a459109d40461a94da8df0c6da4c5e
20 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009947215
21 https://doi.org/10.1007/s002990050430
22 schema:sdDatePublished 2019-04-11T09:57
23 schema:sdLicense https://scigraph.springernature.com/explorer/license/
24 schema:sdPublisher Nc1ff1bb5fafa467aa055bdc239ccf9b7
25 schema:url https://link.springer.com/10.1007%2Fs002990050430
26 sgo:license sg:explorer/license/
27 sgo:sdDataset articles
28 rdf:type schema:ScholarlyArticle
29 N39aaf6169a5f4a8097313cea17a6d050 schema:name nlm_unique_id
30 schema:value 9880970
31 rdf:type schema:PropertyValue
32 N3a18cc99206a4ea9a5ad0037ec3cf67e schema:name readcube_id
33 schema:value 2f6345603d2b227b6a0b59590b3dc03c585841d714096c14a98f5dfbc3c7d591
34 rdf:type schema:PropertyValue
35 N3e7eb80cd4174579ba0aa379aa920555 schema:affiliation https://www.grid.ac/institutes/grid.418554.9
36 schema:familyName Turner
37 schema:givenName J. C.
38 rdf:type schema:Person
39 N4c89520deaf94cfd980c8cfa20d533b7 schema:issueNumber 6-7
40 rdf:type schema:PublicationIssue
41 N5d6149876171408db143c95eefeba4cb rdf:first N3e7eb80cd4174579ba0aa379aa920555
42 rdf:rest rdf:nil
43 N5d9d3547dc7a44fd8048cd37e4e8508f rdf:first sg:person.01051134614.91
44 rdf:rest N683e8c9daa654dfd896c5047d606ad43
45 N683e8c9daa654dfd896c5047d606ad43 rdf:first sg:person.01247363731.84
46 rdf:rest N5d6149876171408db143c95eefeba4cb
47 N6d511bb5bbf14095bb47887cac1f11bf schema:volumeNumber 17
48 rdf:type schema:PublicationVolume
49 N8fd1a4ab6a034353b70cffeebced6bde schema:name pubmed_id
50 schema:value 30736624
51 rdf:type schema:PropertyValue
52 Nc1ff1bb5fafa467aa055bdc239ccf9b7 schema:name Springer Nature - SN SciGraph project
53 rdf:type schema:Organization
54 Ncc16f37c7bdb488da7646a9cf86fa4f5 rdf:first sg:person.0710075556.11
55 rdf:rest N5d9d3547dc7a44fd8048cd37e4e8508f
56 Nd904daad24cc4887b7eaa9bd01143e9e schema:name doi
57 schema:value 10.1007/s002990050430
58 rdf:type schema:PropertyValue
59 Ne3a459109d40461a94da8df0c6da4c5e schema:name dimensions_id
60 schema:value pub.1009947215
61 rdf:type schema:PropertyValue
62 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
63 schema:name Biological Sciences
64 rdf:type schema:DefinedTerm
65 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
66 schema:name Genetics
67 rdf:type schema:DefinedTerm
68 sg:journal.1124809 schema:issn 0721-7714
69 1432-203X
70 schema:name Plant Cell Reports
71 rdf:type schema:Periodical
72 sg:person.01051134614.91 schema:affiliation https://www.grid.ac/institutes/grid.418554.9
73 schema:familyName Knauf
74 schema:givenName V. C.
75 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051134614.91
76 rdf:type schema:Person
77 sg:person.01247363731.84 schema:affiliation https://www.grid.ac/institutes/grid.418554.9
78 schema:familyName Summerfelt
79 schema:givenName K. R.
80 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247363731.84
81 rdf:type schema:Person
82 sg:person.0710075556.11 schema:affiliation https://www.grid.ac/institutes/grid.418554.9
83 schema:familyName Daley
84 schema:givenName M.
85 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0710075556.11
86 rdf:type schema:Person
87 https://www.grid.ac/institutes/grid.418554.9 schema:alternateName Monsanto (United States)
88 schema:name Calgene Inc., 1920 Fifth St., Davis, CA 95616, USA, US
89 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...