Co-transformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-04

AUTHORS

M. Daley, V. C. Knauf, K. R. Summerfelt, J. C. Turner

ABSTRACT

Co-transformation was investigated as a method that would allow the use of a selectable marker during plant regeneration followed by recovery of progeny which contain the desired gene(s) but lack a marker gene. Rapeseed (Brassica napus cv `212/86') and tobacco (Nicotiana tabacum cv `Xanthi NC') were co-cultivated with a single Agrobacterium tumefaciens strain containing two binary plasmids. Genes from both plasmids were expressed in approximately 50% of the primary transformants. Progeny expressing only one of the transgenes were observed in about 50% of the co-transformed lines, indicating that the genes were inserted at different loci. This single-strain co-transformation method allowed the use of a selectable marker during plant regeneration and subsequent recovery of marker-free progeny. More... »

PAGES

489-496

Journal

TITLE

Plant Cell Reports

ISSUE

6-7

VOLUME

17

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002990050430

DOI

http://dx.doi.org/10.1007/s002990050430

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009947215

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30736624


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