Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-11

AUTHORS

R. M. Pérez, L. Navarro, N. Duran-Vila

ABSTRACT

Nucellus-derived embryogenic callus cultures of Salustiana sweet orange were subjected to cryoconservation assays. Cryoprotection with 10%(vol/vol) dimethylsulfoxide, freezing by slow cooling and thawing by fast warming was suitable to recover viable growing cultures and whole plants through embryogenesis. Evaluation of liquid phase R 1 and solid phase R 2 cooling rates using a programmable freezing unit indicated that 100% of embryogenic cultures survived when frozen using a range of cooling rates (R 1 not above 0.5°C min-1 and R 2 not above 1°C min 1) and thawed by fast warming. Storage up to 2 years in liquid nitrogen did not affect the growth of the cryopreserved cultures and the recovery of whole plants. Cultures of four cultivars of sweet orange (C. sinensis Osb.), three cultivars of grapefruit (C. paradisi Macf.), and one cultivar each of lemon [C. limon (L.) Burm. f.], Cleopatra mandarin (C. reshni Hort. ex Tan.), sour orange (C. aurantium L.) and Mexican lime [C. aurantifolia (Christm.) Swing.] have been successfully cryopreserved. Problems using a viability assessment using fluorescein diacetate staining are discussed. More... »

PAGES

44-49

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002990050349

DOI

http://dx.doi.org/10.1007/s002990050349

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1039865471

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30732418


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0915", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Interdisciplinary Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/09", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Engineering", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Instituto Valenciano de Investigaciones Agrarias", 
          "id": "https://www.grid.ac/institutes/grid.419276.f", 
          "name": [
            "Departamento de Protecci\u00f3n Vegetal y Biotecnolog\u00eda, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial, E-46113 Moncada (Valencia), Spain Fax no.: +34-6 1390240 Email: nduran@master.ivia.es, ES"
          ], 
          "type": "Organization"
        }, 
        "familyName": "P\u00e9rez", 
        "givenName": "R. M.", 
        "id": "sg:person.07574447625.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07574447625.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Instituto Valenciano de Investigaciones Agrarias", 
          "id": "https://www.grid.ac/institutes/grid.419276.f", 
          "name": [
            "Departamento de Protecci\u00f3n Vegetal y Biotecnolog\u00eda, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial, E-46113 Moncada (Valencia), Spain Fax no.: +34-6 1390240 Email: nduran@master.ivia.es, ES"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Navarro", 
        "givenName": "L.", 
        "id": "sg:person.01337025443.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337025443.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Instituto Valenciano de Investigaciones Agrarias", 
          "id": "https://www.grid.ac/institutes/grid.419276.f", 
          "name": [
            "Departamento de Protecci\u00f3n Vegetal y Biotecnolog\u00eda, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial, E-46113 Moncada (Valencia), Spain Fax no.: +34-6 1390240 Email: nduran@master.ivia.es, ES"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Duran-Vila", 
        "givenName": "N.", 
        "id": "sg:person.01037236217.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037236217.46"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1997-11", 
    "datePublishedReg": "1997-11-01", 
    "description": "Nucellus-derived embryogenic callus cultures of Salustiana sweet orange were subjected to cryoconservation assays. Cryoprotection with 10%(vol/vol) dimethylsulfoxide, freezing by slow cooling and thawing by fast warming was suitable to recover viable growing cultures and whole plants through embryogenesis. Evaluation of liquid phase R 1 and solid phase R 2 cooling rates using a programmable freezing unit indicated that 100% of embryogenic cultures survived when frozen using a range of cooling rates (R 1 not above 0.5\u00b0C min-1 and R 2 not above 1\u00b0C min 1) and thawed by fast warming. Storage up to 2 years in liquid nitrogen did not affect the growth of the cryopreserved cultures and the recovery of whole plants. Cultures of four cultivars of sweet orange (C. sinensis Osb.), three cultivars of grapefruit (C. paradisi Macf.), and one cultivar each of lemon [C. limon (L.) Burm. f.], Cleopatra mandarin (C. reshni Hort. ex Tan.), sour orange (C. aurantium L.) and Mexican lime [C. aurantifolia (Christm.) Swing.] have been successfully cryopreserved. Problems using a viability assessment using fluorescein diacetate staining are discussed.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s002990050349", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1124809", 
        "issn": [
          "0721-7714", 
          "1432-203X"
        ], 
        "name": "Plant Cell Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "17"
      }
    ], 
    "name": "Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars", 
    "pagination": "44-49", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "bde6762d89638739a6ad8cebe08c8f27bb21c260ff22a047cbe98bad1839c221"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30732418"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9880970"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s002990050349"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1039865471"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s002990050349", 
      "https://app.dimensions.ai/details/publication/pub.1039865471"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:38", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000349_0000000349/records_113677_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs002990050349"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002990050349'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002990050349'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002990050349'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002990050349'


 

This table displays all metadata directly associated to this object as RDF triples.

83 TRIPLES      20 PREDICATES      29 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s002990050349 schema:about anzsrc-for:09
2 anzsrc-for:0915
3 schema:author N7ee1301e9db54551a6a38e034708e9d7
4 schema:datePublished 1997-11
5 schema:datePublishedReg 1997-11-01
6 schema:description Nucellus-derived embryogenic callus cultures of Salustiana sweet orange were subjected to cryoconservation assays. Cryoprotection with 10%(vol/vol) dimethylsulfoxide, freezing by slow cooling and thawing by fast warming was suitable to recover viable growing cultures and whole plants through embryogenesis. Evaluation of liquid phase R <sub>1</sub> and solid phase R <sub>2</sub> cooling rates using a programmable freezing unit indicated that 100% of embryogenic cultures survived when frozen using a range of cooling rates (R <sub>1</sub> not above 0.5°C min<sup>-1</sup> and R <sub>2</sub> not above 1°C min <sup>1</sup>) and thawed by fast warming. Storage up to 2 years in liquid nitrogen did not affect the growth of the cryopreserved cultures and the recovery of whole plants. Cultures of four cultivars of sweet orange (C. sinensis Osb.), three cultivars of grapefruit (C. paradisi Macf.), and one cultivar each of lemon [C. limon (L.) Burm. f.], Cleopatra mandarin (C. reshni Hort. ex Tan.), sour orange (C. aurantium L.) and Mexican lime [C. aurantifolia (Christm.) Swing.] have been successfully cryopreserved. Problems using a viability assessment using fluorescein diacetate staining are discussed.
7 schema:genre research_article
8 schema:inLanguage en
9 schema:isAccessibleForFree false
10 schema:isPartOf N4705c1c6528148ff98f7e680d78ac62b
11 N5d332bd8f8954933ac4bbb074a122780
12 sg:journal.1124809
13 schema:name Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars
14 schema:pagination 44-49
15 schema:productId N425ed314f38542329c17f894e511e6af
16 N58fe5d45bd9c46c5a4700f2e6a5dfceb
17 Nb311ed4f8e354e7791a33fd48d94d7af
18 Nd32871e9fe644326a26a7ca0a4dd9c64
19 Nfe1da5e3213c4f12b756b8b91161fc1f
20 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039865471
21 https://doi.org/10.1007/s002990050349
22 schema:sdDatePublished 2019-04-11T10:38
23 schema:sdLicense https://scigraph.springernature.com/explorer/license/
24 schema:sdPublisher N4a15918302d0468ebb3be90be9510d1f
25 schema:url https://link.springer.com/10.1007%2Fs002990050349
26 sgo:license sg:explorer/license/
27 sgo:sdDataset articles
28 rdf:type schema:ScholarlyArticle
29 N425ed314f38542329c17f894e511e6af schema:name doi
30 schema:value 10.1007/s002990050349
31 rdf:type schema:PropertyValue
32 N4705c1c6528148ff98f7e680d78ac62b schema:issueNumber 1
33 rdf:type schema:PublicationIssue
34 N4a15918302d0468ebb3be90be9510d1f schema:name Springer Nature - SN SciGraph project
35 rdf:type schema:Organization
36 N58fe5d45bd9c46c5a4700f2e6a5dfceb schema:name nlm_unique_id
37 schema:value 9880970
38 rdf:type schema:PropertyValue
39 N5d332bd8f8954933ac4bbb074a122780 schema:volumeNumber 17
40 rdf:type schema:PublicationVolume
41 N7ee1301e9db54551a6a38e034708e9d7 rdf:first sg:person.07574447625.92
42 rdf:rest N817f285e1d354ed3bed06655f26a3207
43 N817f285e1d354ed3bed06655f26a3207 rdf:first sg:person.01337025443.76
44 rdf:rest Nba00b64d534f4ff7b010ae4ce6799185
45 Nb311ed4f8e354e7791a33fd48d94d7af schema:name dimensions_id
46 schema:value pub.1039865471
47 rdf:type schema:PropertyValue
48 Nba00b64d534f4ff7b010ae4ce6799185 rdf:first sg:person.01037236217.46
49 rdf:rest rdf:nil
50 Nd32871e9fe644326a26a7ca0a4dd9c64 schema:name pubmed_id
51 schema:value 30732418
52 rdf:type schema:PropertyValue
53 Nfe1da5e3213c4f12b756b8b91161fc1f schema:name readcube_id
54 schema:value bde6762d89638739a6ad8cebe08c8f27bb21c260ff22a047cbe98bad1839c221
55 rdf:type schema:PropertyValue
56 anzsrc-for:09 schema:inDefinedTermSet anzsrc-for:
57 schema:name Engineering
58 rdf:type schema:DefinedTerm
59 anzsrc-for:0915 schema:inDefinedTermSet anzsrc-for:
60 schema:name Interdisciplinary Engineering
61 rdf:type schema:DefinedTerm
62 sg:journal.1124809 schema:issn 0721-7714
63 1432-203X
64 schema:name Plant Cell Reports
65 rdf:type schema:Periodical
66 sg:person.01037236217.46 schema:affiliation https://www.grid.ac/institutes/grid.419276.f
67 schema:familyName Duran-Vila
68 schema:givenName N.
69 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037236217.46
70 rdf:type schema:Person
71 sg:person.01337025443.76 schema:affiliation https://www.grid.ac/institutes/grid.419276.f
72 schema:familyName Navarro
73 schema:givenName L.
74 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337025443.76
75 rdf:type schema:Person
76 sg:person.07574447625.92 schema:affiliation https://www.grid.ac/institutes/grid.419276.f
77 schema:familyName Pérez
78 schema:givenName R. M.
79 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07574447625.92
80 rdf:type schema:Person
81 https://www.grid.ac/institutes/grid.419276.f schema:alternateName Instituto Valenciano de Investigaciones Agrarias
82 schema:name Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial, E-46113 Moncada (Valencia), Spain Fax no.: +34-6 1390240 Email: nduran@master.ivia.es, ES
83 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...