Construction of a GFP-BAR plasmid and its use for switchgrass transformation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-01

AUTHORS

H. A. Richards, V. A. Rudas, H. Sun, J. K. McDaniel, Z. Tomaszewski, B. V. Conger

ABSTRACT

A dual marker plasmid comprising the reporter gene sgfp (green fluorescent protein) and the selectable bar gene (Basta tolerance) was constructed by replacing the uidA (β-glucuronidase, GUS) gene in a uidA-bar construct with sgfp. A particle inflow gun was used to propel tungsten particles coated with this plasmid into immature inflorescence-derived embryogenic callus of switchgrass (Panicum virgatum L.). GFP was observed in leaf tissue and pollen of transgenic plants. Nearly 100 plants tolerant to Basta were obtained from the experiments, and Southern blot hybridization confirmed the presence of both the bar and sgfp genes. Plants regenerated from in vitro cultures of transgenic plants grew on medium with 10 mg l-1 bialaphos. When the pH indicator chlorophenol red was in the medium, the transgenic plantlets changed the medium from red to yellow. Basta tolerance was observed in T1 plants resulting from crosses between transgenic and nontransgenic control plants, indicating inheritance of the bar transgene. More... »

PAGES

48-54

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002990000274

DOI

http://dx.doi.org/10.1007/s002990000274

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041110776

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30759912


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