Transformation of barley scutellum protoplasts: regeneration of fertile transgenic plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-10

AUTHORS

J. Nobre, M. R. Davey, P. A. Lazzeri, M. E. Cannell

ABSTRACT

A system for barley transformation via polyethyleneglycol-mediated DNA uptake into protoplasts isolated directly from scutella and the regeneration of transgenic plants is reported. Scutellum protoplasts (cv. Clipper, an Australian malting cultivar) were co-transformed with plasmids Act 1-DGUS, containing the marker uidA gene, and pCaIneo, which contains the selectable marker neomycin phosphotransferase gene. Protoplast-derived calluses were selected on medium containing the antibiotic G418 (25 and 15 mg.l-1) and macroscopic antibiotic resistant colonies were recovered. Fertile plants were regenerated from a callus line and molecular analysis confirmed transgene integration. More... »

PAGES

1000-1005

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002990000220

DOI

http://dx.doi.org/10.1007/s002990000220

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020078970

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30754830


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0703", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Crop and Pasture Production", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/07", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Agricultural and Veterinary Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Rothamsted Research", 
          "id": "https://www.grid.ac/institutes/grid.418374.d", 
          "name": [
            "Department of Biochemistry and Physiology, IACR-Rothamsted, Harpenden, AL5 2JQ, UK, GB"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nobre", 
        "givenName": "J.", 
        "id": "sg:person.016111045351.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016111045351.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Nottingham", 
          "id": "https://www.grid.ac/institutes/grid.4563.4", 
          "name": [
            "Plant Science Division, School of Biological Sciences University of Nottingham, University Park, Nottingham, NG7 2RD, UK, GB"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Davey", 
        "givenName": "M. R.", 
        "id": "sg:person.01264611513.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01264611513.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Rothamsted Research", 
          "id": "https://www.grid.ac/institutes/grid.418374.d", 
          "name": [
            "DuPont CIC \u2013 Wheat Transformation Laboratory, c/o IACR-Rothamsted, Harpenden, AL5 2JQ, UK, GB"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lazzeri", 
        "givenName": "P. A.", 
        "id": "sg:person.0674437245.89", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0674437245.89"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Rothamsted Research", 
          "id": "https://www.grid.ac/institutes/grid.418374.d", 
          "name": [
            "Department of Biochemistry and Physiology, IACR-Rothamsted, Harpenden, AL5 2JQ, UK, GB"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cannell", 
        "givenName": "M. E.", 
        "id": "sg:person.0764411411.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764411411.15"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2000-10", 
    "datePublishedReg": "2000-10-01", 
    "description": "\u2002A system for barley transformation via polyethyleneglycol-mediated DNA uptake into protoplasts isolated directly from scutella and the regeneration of transgenic plants is reported. Scutellum protoplasts (cv. Clipper, an Australian malting cultivar) were co-transformed with plasmids Act 1-DGUS, containing the marker uidA gene, and pCaIneo, which contains the selectable marker neomycin phosphotransferase gene. Protoplast-derived calluses were selected on medium containing the antibiotic G418 (25 and 15\u2009mg.l-1) and macroscopic antibiotic resistant colonies were recovered. Fertile plants were regenerated from a callus line and molecular analysis confirmed transgene integration.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s002990000220", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1124809", 
        "issn": [
          "0721-7714", 
          "1432-203X"
        ], 
        "name": "Plant Cell Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "19"
      }
    ], 
    "name": "Transformation of barley scutellum protoplasts: regeneration of fertile transgenic plants", 
    "pagination": "1000-1005", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "5fab345a83497b3ab56f36e9c5900d85cc5c102ac69e6ba8531a1fabf92340b2"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30754830"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9880970"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s002990000220"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1020078970"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s002990000220", 
      "https://app.dimensions.ai/details/publication/pub.1020078970"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:59", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000347_0000000347/records_89814_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs002990000220"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002990000220'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002990000220'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002990000220'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002990000220'


 

This table displays all metadata directly associated to this object as RDF triples.

94 TRIPLES      20 PREDICATES      29 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s002990000220 schema:about anzsrc-for:07
2 anzsrc-for:0703
3 schema:author N88065f76a7624cb2803225f5ddc0c22e
4 schema:datePublished 2000-10
5 schema:datePublishedReg 2000-10-01
6 schema:description  A system for barley transformation via polyethyleneglycol-mediated DNA uptake into protoplasts isolated directly from scutella and the regeneration of transgenic plants is reported. Scutellum protoplasts (cv. Clipper, an Australian malting cultivar) were co-transformed with plasmids Act 1-DGUS, containing the marker uidA gene, and pCaIneo, which contains the selectable marker neomycin phosphotransferase gene. Protoplast-derived calluses were selected on medium containing the antibiotic G418 (25 and 15 mg.l<sup>-1</sup>) and macroscopic antibiotic resistant colonies were recovered. Fertile plants were regenerated from a callus line and molecular analysis confirmed transgene integration.
7 schema:genre research_article
8 schema:inLanguage en
9 schema:isAccessibleForFree false
10 schema:isPartOf N405e537e9bdb4dccbce67b03489f2f14
11 N4b0d415433c74f9ca3b12ba85632bfd7
12 sg:journal.1124809
13 schema:name Transformation of barley scutellum protoplasts: regeneration of fertile transgenic plants
14 schema:pagination 1000-1005
15 schema:productId Ne0fcae228dc34fc79d8273f60cbdeee3
16 Ne3db1f2e14014e2c8cce2d3bb0c0af55
17 Ne416d4ae4d5c4d8bbee7e5f4b3fd59b5
18 Nf1bee4cd4e3546b494c3b8713e80a78d
19 Nfe8e04b35dac4faca86c05b81bf3fe73
20 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020078970
21 https://doi.org/10.1007/s002990000220
22 schema:sdDatePublished 2019-04-11T09:59
23 schema:sdLicense https://scigraph.springernature.com/explorer/license/
24 schema:sdPublisher N5bfe0246cecf4c3b915de8782afab2b9
25 schema:url https://link.springer.com/10.1007%2Fs002990000220
26 sgo:license sg:explorer/license/
27 sgo:sdDataset articles
28 rdf:type schema:ScholarlyArticle
29 N39411180cc06483580d21f7dc68ea322 rdf:first sg:person.0674437245.89
30 rdf:rest N9392b061da3d4c31a3b3e1f20db21ab8
31 N405e537e9bdb4dccbce67b03489f2f14 schema:issueNumber 10
32 rdf:type schema:PublicationIssue
33 N46eb6a8563604deb875d74890783aa89 rdf:first sg:person.01264611513.23
34 rdf:rest N39411180cc06483580d21f7dc68ea322
35 N4b0d415433c74f9ca3b12ba85632bfd7 schema:volumeNumber 19
36 rdf:type schema:PublicationVolume
37 N5bfe0246cecf4c3b915de8782afab2b9 schema:name Springer Nature - SN SciGraph project
38 rdf:type schema:Organization
39 N88065f76a7624cb2803225f5ddc0c22e rdf:first sg:person.016111045351.07
40 rdf:rest N46eb6a8563604deb875d74890783aa89
41 N9392b061da3d4c31a3b3e1f20db21ab8 rdf:first sg:person.0764411411.15
42 rdf:rest rdf:nil
43 Ne0fcae228dc34fc79d8273f60cbdeee3 schema:name readcube_id
44 schema:value 5fab345a83497b3ab56f36e9c5900d85cc5c102ac69e6ba8531a1fabf92340b2
45 rdf:type schema:PropertyValue
46 Ne3db1f2e14014e2c8cce2d3bb0c0af55 schema:name dimensions_id
47 schema:value pub.1020078970
48 rdf:type schema:PropertyValue
49 Ne416d4ae4d5c4d8bbee7e5f4b3fd59b5 schema:name pubmed_id
50 schema:value 30754830
51 rdf:type schema:PropertyValue
52 Nf1bee4cd4e3546b494c3b8713e80a78d schema:name nlm_unique_id
53 schema:value 9880970
54 rdf:type schema:PropertyValue
55 Nfe8e04b35dac4faca86c05b81bf3fe73 schema:name doi
56 schema:value 10.1007/s002990000220
57 rdf:type schema:PropertyValue
58 anzsrc-for:07 schema:inDefinedTermSet anzsrc-for:
59 schema:name Agricultural and Veterinary Sciences
60 rdf:type schema:DefinedTerm
61 anzsrc-for:0703 schema:inDefinedTermSet anzsrc-for:
62 schema:name Crop and Pasture Production
63 rdf:type schema:DefinedTerm
64 sg:journal.1124809 schema:issn 0721-7714
65 1432-203X
66 schema:name Plant Cell Reports
67 rdf:type schema:Periodical
68 sg:person.01264611513.23 schema:affiliation https://www.grid.ac/institutes/grid.4563.4
69 schema:familyName Davey
70 schema:givenName M. R.
71 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01264611513.23
72 rdf:type schema:Person
73 sg:person.016111045351.07 schema:affiliation https://www.grid.ac/institutes/grid.418374.d
74 schema:familyName Nobre
75 schema:givenName J.
76 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016111045351.07
77 rdf:type schema:Person
78 sg:person.0674437245.89 schema:affiliation https://www.grid.ac/institutes/grid.418374.d
79 schema:familyName Lazzeri
80 schema:givenName P. A.
81 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0674437245.89
82 rdf:type schema:Person
83 sg:person.0764411411.15 schema:affiliation https://www.grid.ac/institutes/grid.418374.d
84 schema:familyName Cannell
85 schema:givenName M. E.
86 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764411411.15
87 rdf:type schema:Person
88 https://www.grid.ac/institutes/grid.418374.d schema:alternateName Rothamsted Research
89 schema:name Department of Biochemistry and Physiology, IACR-Rothamsted, Harpenden, AL5 2JQ, UK, GB
90 DuPont CIC – Wheat Transformation Laboratory, c/o IACR-Rothamsted, Harpenden, AL5 2JQ, UK, GB
91 rdf:type schema:Organization
92 https://www.grid.ac/institutes/grid.4563.4 schema:alternateName University of Nottingham
93 schema:name Plant Science Division, School of Biological Sciences University of Nottingham, University Park, Nottingham, NG7 2RD, UK, GB
94 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...