Agrobacterium-mediated transformation of monocot and dicot plants using the NCR promoter derived from soybean chlorotic mottle virus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-07

AUTHORS

H. Fukuoka, T. Ogawa, I. Mitsuhara, T. Iwai, K. Isuzugawa, Yoko Nishizawa, Y. Gotoh, Yaeko Nishizawa, A. Tagiri, M. Ugaki, M. Ohshima, H. Yano, N. Murai, Y. Niwa, T. Hibi, Y. Ohashi

ABSTRACT

The NCR promoter (PNCR) from soybean chlorotic mottle virus (SoyCMV) was used to express the selectable marker, neomycin phosphotransferase (nptII) gene, in Agrobacterium-mediated transformation of both monocot (rice) and dicot (tobacco) plants. A multi-cloning site for insertion of a gene of interest into the binary vector pTN is located proximal to the right border region of T-DNA. When chimeric genes under the control of other strong promoters were located in a head-to-head orientation to the PNCR-nptII gene, kanamycin-resistant tobacco shoots were generated more efficiently than when using the original pTN vectors. This suggests that the enhancer-like sequences in the promoters adjacent to PNCR may promote expression of the PNCR-nptII gene. More... »

PAGES

815-820

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002990000191

DOI

http://dx.doi.org/10.1007/s002990000191

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052464872

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30754875


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