Chlorophyll fluorescence lifetime imaging provides new insight into the chlorosis induced by plant virus infection View Full Text


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Article Info

DATE

2016-11-30

AUTHORS

Rong Lei, Hongshan Jiang, Fan Hu, Jin Yan, Shuifang Zhu

ABSTRACT

Key messageLeaf chlorosis induced by plant virus infection has a short fluorescence lifetime, which reflects damaged photosynthetic complexes and degraded chloroplasts.AbstractPlant viruses often induce chlorosis and necrosis, which are intimately related to photosynthetic functions. Chlorophyll fluorescence lifetime measurement is a valuable noninvasive tool for analyzing photosynthetic processes and is a sensitive indicator of the environment surrounding the fluorescent molecules. In this study, our central goal was to explore the effect of viral infection on photosynthesis by employing chlorophyll fluorescence lifetime imaging (FLIM), steady-state fluorescence, non-photochemical quenching (NPQ), transmission electron microscopy (TEM), and pigment analysis. The data indicated that the chlorophyll fluorescence lifetime of chlorotic leaves was significantly shorter than that of healthy control leaves, and the fitted short lifetime component of chlorophyll fluorescence of chlorotic leaves was dominant. This dominant short lifetime component may result from damage to the structure of thylakoid, which was confirmed by TEM. The NPQ value of chlorotic leaves was slightly higher than that of healthy green leaves, which can be explained by increased neoxanthin, lutein and violaxanthin content relative to chlorophyll a. The difference in NPQ is slight, but FLIM can provide simple and direct characterization of PSII structure and photosynthetic function. Therefore, this technique shows great potential as a simple and rapid method for studying mechanisms of plant virus infection. More... »

PAGES

327-341

References to SciGraph publications

  • 2015-03-26. A megacomplex composed of both photosystem reaction centres in higher plants in NATURE COMMUNICATIONS
  • 2003-09. Photosynthesis and Activity of Phosphoenolpyruvate carboxylase in Nicotiana tabacum L. Leaves Infected by Potato virus A and Potato virus Y in PHOTOSYNTHETICA
  • 1992-06. Chlorophyll fluorescence lifetime determination of waterstressed C3- and C4-plants in RADIATION AND ENVIRONMENTAL BIOPHYSICS
  • 2012-07-18. Two-photon excitation chlorophyll fluorescence lifetime imaging: a rapid and noninvasive method for in vivo assessment of cadmium toxicity in a marine diatom Thalassiosira weissflogii in PLANTA
  • 2000-01. A pigment-binding protein essential for regulation of photosynthetic light harvesting in NATURE
  • 2006-06-29. Infection with virulent and avirulent P. syringae strains differentially affects photosynthesis and sink metabolism in Arabidopsis leaves in PLANTA
  • 1986-12. Time-resolved fluorescence of conifers exposed to environmental pollutants in RADIATION AND ENVIRONMENTAL BIOPHYSICS
  • 1991-04. A protein in the oxygen-evolving complex in the chloroplast is associated with symptom expression on tobacco leaves infected with cucumber mosaic virus strain Y in PLANT MOLECULAR BIOLOGY
  • 2007-11. Identification of a mechanism of photoprotective energy dissipation in higher plants in NATURE
  • 1996-05. Photosystem II chlorophyll a fluorescence lifetimes and intensity are independent of the antenna size differences between barley wild-type and chlorina mutants: Photochemical quenching and xanthophyll cycle-dependent nonphotochemical quenching of fluorescence in PHOTOSYNTHESIS RESEARCH
  • 2000-08. Fluorescence Lifetime Imaging (FLI) in Real-Time - a New Technique in Photosynthesis Research in PHOTOSYNTHETICA
  • 2013-03-15. Chlorophyll a fluorescence as a tool for a study of the Potato virus Y effects on photosynthesis of nontransgenic and transgenic Pssu-ipt tobacco in PHOTOSYNTHETICA
  • 2008-09-16. Conventional and combinatorial chlorophyll fluorescence imaging of tobamovirus-infected plants in PHOTOSYNTHETICA
  • 2008-02-15. Chlorophyll fluorescence emission spectrum inside a leaf in PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
  • 2009-05-26. Applying two-photon excitation fluorescence lifetime imaging microscopy to study photosynthesis in plant leaves in PHOTOSYNTHESIS RESEARCH
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    http://scigraph.springernature.com/pub.10.1007/s00299-016-2083-y

    DOI

    http://dx.doi.org/10.1007/s00299-016-2083-y

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27904946


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    91 method
    92 microscopy
    93 molecules
    94 necrosis
    95 neoxanthin
    96 new insights
    97 non-photochemical quenching
    98 noninvasive tool
    99 photosynthesis
    100 photosynthetic complexes
    101 photosynthetic function
    102 photosynthetic processes
    103 pigment analysis
    104 plant virus infection
    105 potential
    106 process
    107 quenching
    108 rapid method
    109 sensitive indicator
    110 short fluorescence lifetime
    111 short lifetime component
    112 steady-state fluorescence
    113 structure
    114 structure of thylakoids
    115 study
    116 technique
    117 thylakoids
    118 tool
    119 transmission electron microscopy
    120 valuable noninvasive tool
    121 values
    122 violaxanthin content
    123 viral infection
    124 virus
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