A chimeric cry8Ea1 gene flanked by MARs efficiently controls Holotrichia parallela View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-08

AUTHORS

Lili Geng, Jing Chi, Changlong Shu, Peter M. Gresshoff, Fuping Song, Dafang Huang, Jie Zhang

ABSTRACT

KEY MESSAGE: Peanuts transformed with the synthetic cry8Ea1 gene flanked by MARs are a potentially effective control strategy against white grubs. Cry8Ea1 protein levels of the construct containing MARs were increased by 2.5 times. White grubs are now recognized as the most important pests of peanut worldwide. A synthetic cry8Ea1 gene, which was toxic to Holotrichia parallela larvae, was expressed in chimeric peanut roots using an Agrobacterium rhizogenes-mediated transformation system. The relative mRNA and protein levels of the cry8Ea1 gene were confirmed by quantitative real-time PCR and ELISA, respectively. The effects of matrix attachment regions (MARs) on the expression and activity of the cry8Ea1 gene were analyzed. The average expression level of cry8Ea1 in peanut roots was higher for the plants harboring constructs flanked by MARs from tobacco. Moreover, differing from previous studies, the synthetic cry8Ea1 gene flanked by MARs showed more variation in protein levels than mRNA levels. These composite plants containing cry8Ea1 gene flanked by MARs exhibited a high toxicity against Holotrichia parallela larvae as shown by bioassay analysis, thus offering a potential effective combination to control subterranean insects in peanuts. More... »

PAGES

1211-1218

References to SciGraph publications

  • 2009-06. Functional characterization of a tobacco matrix attachment region-mediated enhancement of transgene expression in TRANSGENIC RESEARCH
  • 2009-04. Characterization of Two Novel cry8 Genes from Bacillus thuringiensis Strain BT185 in CURRENT MICROBIOLOGY
  • 2012-01. Minimizing the unpredictability of transgene expression in plants: the role of genetic insulators in PLANT CELL REPORTS
  • 2012-06. Efficient production of Agrobacterium rhizogenes-transformed roots and composite plants in peanut (Arachis hypogaea L.) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2011-06. An Improved Bioassay Method for Entomopathogenic Fungi of Sugarcane Pests and Its Evaluation in Studies of Virulence in Subcultures in SUGAR TECH
  • 2009. Use of MAR Elements to Increase the Production of Recombinant Proteins in CELL LINE DEVELOPMENT
  • 2008-06. Expression of a synthetic cry1EC gene for resistance against Spodoptera litura in transgenic peanut (Arachis hypogaea L.) in PLANT CELL REPORTS
  • 2010-06. Construction of a Bacillus thuringiensis engineered strain with high toxicity and broad pesticidal spectrum against coleopteran insects in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2007-09. Increased expression of transgene in stably transformed cells of Dunaliella salina by matrix attachment regions in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2011-09-21. Molecular Farming in Plants: An Overview in MOLECULAR FARMING IN PLANTS: RECENT ADVANCES AND FUTURE PROSPECTS
  • 2007-04. Agrobacterium rhizogenes-mediated transformation of soybean to study root biology in NATURE PROTOCOLS
  • 1994-03. The effect of root damage caused by simulated white grub attack on the growth, yield and water-use of groundnut plants in PLANT AND SOIL
  • 2005-04. Matrix attachment regions increase the efficiency and stability of RNA-mediated resistance to Tomato Spotted Wilt Virus in transgenic tobacco in TRANSGENIC RESEARCH
  • 1997-03. Expression of a Bacillus thuringiensis cryIA(c) gene in transgenic peanut plants and its efficacy against lesser cornstalk borer in TRANSGENIC RESEARCH
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00299-013-1417-2

    DOI

    http://dx.doi.org/10.1007/s00299-013-1417-2

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1009966337

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23535868


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Arachis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biological Assay", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Coleoptera", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Matrix Attachment Regions", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plant Roots", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants, Genetically Modified", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombinant Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transcription, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transformation, Genetic", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Institute of Plant Protection", 
              "id": "https://www.grid.ac/institutes/grid.464356.6", 
              "name": [
                "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Geng", 
            "givenName": "Lili", 
            "id": "sg:person.0743360071.96", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0743360071.96"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Plant Protection", 
              "id": "https://www.grid.ac/institutes/grid.464356.6", 
              "name": [
                "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chi", 
            "givenName": "Jing", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Plant Protection", 
              "id": "https://www.grid.ac/institutes/grid.464356.6", 
              "name": [
                "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shu", 
            "givenName": "Changlong", 
            "id": "sg:person.0662453611.56", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662453611.56"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Queensland", 
              "id": "https://www.grid.ac/institutes/grid.1003.2", 
              "name": [
                "ARC Centre of Excellence for Integrative Legume Research, The University of Queensland, St. Lucia, 4072, Brisbane, QLD, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gresshoff", 
            "givenName": "Peter M.", 
            "id": "sg:person.01363624355.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363624355.34"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Plant Protection", 
              "id": "https://www.grid.ac/institutes/grid.464356.6", 
              "name": [
                "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Song", 
            "givenName": "Fuping", 
            "id": "sg:person.01270102723.28", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270102723.28"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Biotechnology Research Institute", 
              "id": "https://www.grid.ac/institutes/grid.418873.1", 
              "name": [
                "Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Huang", 
            "givenName": "Dafang", 
            "id": "sg:person.0727054501.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727054501.27"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Plant Protection", 
              "id": "https://www.grid.ac/institutes/grid.464356.6", 
              "name": [
                "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Jie", 
            "id": "sg:person.01105541123.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105541123.50"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1111/j.1365-313x.2010.04398.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001982211"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00299-008-0525-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004357149", 
              "https://doi.org/10.1007/s00299-008-0525-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00299-008-0525-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004357149", 
              "https://doi.org/10.1007/s00299-008-0525-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00299-008-0525-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004357149", 
              "https://doi.org/10.1007/s00299-008-0525-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/09670870500399866", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005600480"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-007-1040-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009029709", 
              "https://doi.org/10.1007/s00253-007-1040-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-007-1040-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009029709", 
              "https://doi.org/10.1007/s00253-007-1040-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.107.111096", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012493034"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/09583157.2011.566324", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013208090"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/jee/100.2.431", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013848664"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00010152", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014293696", 
              "https://doi.org/10.1007/bf00010152"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00010152", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014293696", 
              "https://doi.org/10.1007/bf00010152"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00284-008-9338-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015190150", 
              "https://doi.org/10.1007/s00284-008-9338-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00284-008-9338-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015190150", 
              "https://doi.org/10.1007/s00284-008-9338-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.10.5.2302", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017428371"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2007.141", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021137406", 
              "https://doi.org/10.1038/nprot.2007.141"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023703429"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/treephys/28.1.55", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025218609"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/ps.1701", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032867062"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-90-481-2245-5_1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033829161", 
              "https://doi.org/10.1007/978-90-481-2245-5_1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12355-011-0080-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035485224", 
              "https://doi.org/10.1007/s12355-011-0080-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1360-1385(98)01198-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037482240"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0261-2194(99)00028-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037915963"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1603/0022-0493(2007)100[431:reoioj]2.0.co;2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041206754"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/ps.2041", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041483727"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00299-011-1167-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042252900", 
              "https://doi.org/10.1007/s00299-011-1167-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00299-011-1167-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042252900", 
              "https://doi.org/10.1007/s00299-011-1167-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-010-2479-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043836914", 
              "https://doi.org/10.1007/s00253-010-2479-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-010-2479-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043836914", 
              "https://doi.org/10.1007/s00253-010-2479-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1018481805928", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045821799", 
              "https://doi.org/10.1023/a:1018481805928"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11248-004-5413-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048508160", 
              "https://doi.org/10.1007/s11248-004-5413-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11248-004-5413-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048508160", 
              "https://doi.org/10.1007/s11248-004-5413-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11240-012-0113-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049226773", 
              "https://doi.org/10.1007/s11240-012-0113-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11248-008-9230-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049252548", 
              "https://doi.org/10.1007/s11248-008-9230-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/aem.01074-07", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049709351"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-94-007-2217-0_1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049764614", 
              "https://doi.org/10.1007/978-94-007-2217-0_1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jip.2007.09.007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053348623"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2013-08", 
        "datePublishedReg": "2013-08-01", 
        "description": "KEY MESSAGE: Peanuts transformed with the synthetic cry8Ea1 gene flanked by MARs are a potentially effective control strategy against white grubs. Cry8Ea1 protein levels of the construct containing MARs were increased by 2.5 times. White grubs are now recognized as the most important pests of peanut worldwide. A synthetic cry8Ea1 gene, which was toxic to Holotrichia parallela larvae, was expressed in chimeric peanut roots using an Agrobacterium rhizogenes-mediated transformation system. The relative mRNA and protein levels of the cry8Ea1 gene were confirmed by quantitative real-time PCR and ELISA, respectively. The effects of matrix attachment regions (MARs) on the expression and activity of the cry8Ea1 gene were analyzed. The average expression level of cry8Ea1 in peanut roots was higher for the plants harboring constructs flanked by MARs from tobacco. Moreover, differing from previous studies, the synthetic cry8Ea1 gene flanked by MARs showed more variation in protein levels than mRNA levels. These composite plants containing cry8Ea1 gene flanked by MARs exhibited a high toxicity against Holotrichia parallela larvae as shown by bioassay analysis, thus offering a potential effective combination to control subterranean insects in peanuts.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s00299-013-1417-2", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1124809", 
            "issn": [
              "0721-7714", 
              "1432-203X"
            ], 
            "name": "Plant Cell Reports", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "8", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "32"
          }
        ], 
        "name": "A chimeric cry8Ea1 gene flanked by MARs efficiently controls Holotrichia parallela", 
        "pagination": "1211-1218", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "2f7aa8d0e5888701b1da14dce8f188a38ef5a49feade1040373a27dca9f9a08a"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "23535868"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9880970"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00299-013-1417-2"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1009966337"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00299-013-1417-2", 
          "https://app.dimensions.ai/details/publication/pub.1009966337"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T09:58", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000347_0000000347/records_89812_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://link.springer.com/10.1007%2Fs00299-013-1417-2"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00299-013-1417-2'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00299-013-1417-2'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00299-013-1417-2'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00299-013-1417-2'


     

    This table displays all metadata directly associated to this object as RDF triples.

    257 TRIPLES      21 PREDICATES      68 URIs      31 LITERALS      19 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00299-013-1417-2 schema:about N5b6eeab344dc4141bfecf5ca0735daa3
    2 N5cee195bd893437b9de73ac18bade12f
    3 N73258230b23845f99e872bc46e2bf254
    4 N8ddc371915f84c2ebf67da1f47708d15
    5 Nbdabbaa3baa64299896e4b61aebc1e27
    6 Nc9c72b8047f841c1b8741766af0d3041
    7 Nd1393a9a3b1b455b8ff1971083f7bfdf
    8 Nd801576fefc84b00803e7a8129e9f312
    9 Ne6479f1215494d48b310947bb9bbb04d
    10 Nff2db9922aa24a48b36467867270feaa
    11 anzsrc-for:06
    12 anzsrc-for:0604
    13 schema:author Ndb97a6cfa2c7449fa97a55df36423c66
    14 schema:citation sg:pub.10.1007/978-90-481-2245-5_1
    15 sg:pub.10.1007/978-94-007-2217-0_1
    16 sg:pub.10.1007/bf00010152
    17 sg:pub.10.1007/s00253-007-1040-7
    18 sg:pub.10.1007/s00253-010-2479-5
    19 sg:pub.10.1007/s00284-008-9338-y
    20 sg:pub.10.1007/s00299-008-0525-x
    21 sg:pub.10.1007/s00299-011-1167-y
    22 sg:pub.10.1007/s11240-012-0113-1
    23 sg:pub.10.1007/s11248-004-5413-8
    24 sg:pub.10.1007/s11248-008-9230-3
    25 sg:pub.10.1007/s12355-011-0080-6
    26 sg:pub.10.1023/a:1018481805928
    27 sg:pub.10.1038/nprot.2007.141
    28 https://doi.org/10.1002/ps.1701
    29 https://doi.org/10.1002/ps.2041
    30 https://doi.org/10.1016/j.jip.2007.09.007
    31 https://doi.org/10.1016/s0261-2194(99)00028-9
    32 https://doi.org/10.1016/s1360-1385(98)01198-4
    33 https://doi.org/10.1080/09583157.2011.566324
    34 https://doi.org/10.1080/09670870500399866
    35 https://doi.org/10.1093/jee/100.2.431
    36 https://doi.org/10.1093/treephys/28.1.55
    37 https://doi.org/10.1104/pp.107.111096
    38 https://doi.org/10.1111/j.1365-313x.2010.04398.x
    39 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
    40 https://doi.org/10.1128/aem.01074-07
    41 https://doi.org/10.1128/mcb.10.5.2302
    42 https://doi.org/10.1603/0022-0493(2007)100[431:reoioj]2.0.co;2
    43 schema:datePublished 2013-08
    44 schema:datePublishedReg 2013-08-01
    45 schema:description KEY MESSAGE: Peanuts transformed with the synthetic cry8Ea1 gene flanked by MARs are a potentially effective control strategy against white grubs. Cry8Ea1 protein levels of the construct containing MARs were increased by 2.5 times. White grubs are now recognized as the most important pests of peanut worldwide. A synthetic cry8Ea1 gene, which was toxic to Holotrichia parallela larvae, was expressed in chimeric peanut roots using an Agrobacterium rhizogenes-mediated transformation system. The relative mRNA and protein levels of the cry8Ea1 gene were confirmed by quantitative real-time PCR and ELISA, respectively. The effects of matrix attachment regions (MARs) on the expression and activity of the cry8Ea1 gene were analyzed. The average expression level of cry8Ea1 in peanut roots was higher for the plants harboring constructs flanked by MARs from tobacco. Moreover, differing from previous studies, the synthetic cry8Ea1 gene flanked by MARs showed more variation in protein levels than mRNA levels. These composite plants containing cry8Ea1 gene flanked by MARs exhibited a high toxicity against Holotrichia parallela larvae as shown by bioassay analysis, thus offering a potential effective combination to control subterranean insects in peanuts.
    46 schema:genre research_article
    47 schema:inLanguage en
    48 schema:isAccessibleForFree false
    49 schema:isPartOf N6de2bd24162b432ba156b11b20120614
    50 Ne29ac12b196948e8aecf1d73407dd333
    51 sg:journal.1124809
    52 schema:name A chimeric cry8Ea1 gene flanked by MARs efficiently controls Holotrichia parallela
    53 schema:pagination 1211-1218
    54 schema:productId N0df2156bd62f49829e2970e1fc7af63d
    55 N319a9e893df14fa1bf6fa90770919979
    56 N36a2d4ca7fc1437290d047ec8b70445e
    57 N3d78c31df8724fbe8226aae1cdf6269b
    58 Nacb6dbf11f3b4799967be9bee4ad2b01
    59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009966337
    60 https://doi.org/10.1007/s00299-013-1417-2
    61 schema:sdDatePublished 2019-04-11T09:58
    62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    63 schema:sdPublisher N23589e2fe3ed4ceca2b0421c9c13b95a
    64 schema:url https://link.springer.com/10.1007%2Fs00299-013-1417-2
    65 sgo:license sg:explorer/license/
    66 sgo:sdDataset articles
    67 rdf:type schema:ScholarlyArticle
    68 N0df2156bd62f49829e2970e1fc7af63d schema:name nlm_unique_id
    69 schema:value 9880970
    70 rdf:type schema:PropertyValue
    71 N23589e2fe3ed4ceca2b0421c9c13b95a schema:name Springer Nature - SN SciGraph project
    72 rdf:type schema:Organization
    73 N319a9e893df14fa1bf6fa90770919979 schema:name doi
    74 schema:value 10.1007/s00299-013-1417-2
    75 rdf:type schema:PropertyValue
    76 N36a2d4ca7fc1437290d047ec8b70445e schema:name readcube_id
    77 schema:value 2f7aa8d0e5888701b1da14dce8f188a38ef5a49feade1040373a27dca9f9a08a
    78 rdf:type schema:PropertyValue
    79 N3d78c31df8724fbe8226aae1cdf6269b schema:name dimensions_id
    80 schema:value pub.1009966337
    81 rdf:type schema:PropertyValue
    82 N5b6eeab344dc4141bfecf5ca0735daa3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    83 schema:name Plants, Genetically Modified
    84 rdf:type schema:DefinedTerm
    85 N5cee195bd893437b9de73ac18bade12f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    86 schema:name Arachis
    87 rdf:type schema:DefinedTerm
    88 N6de2bd24162b432ba156b11b20120614 schema:volumeNumber 32
    89 rdf:type schema:PublicationVolume
    90 N73258230b23845f99e872bc46e2bf254 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    91 schema:name Transformation, Genetic
    92 rdf:type schema:DefinedTerm
    93 N7543c0b2adbe47b2991e430eca8be2e9 rdf:first sg:person.01105541123.50
    94 rdf:rest rdf:nil
    95 N79ad736448b24be9af2a46d1549de01d rdf:first sg:person.0662453611.56
    96 rdf:rest Nb615a5644a3b44d296ca06f2d4edc941
    97 N8ddc371915f84c2ebf67da1f47708d15 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    98 schema:name Biological Assay
    99 rdf:type schema:DefinedTerm
    100 Na770fcbd37c549dda33f32603083bd6b rdf:first sg:person.0727054501.27
    101 rdf:rest N7543c0b2adbe47b2991e430eca8be2e9
    102 Na90276c314cf41a9969870716617536a rdf:first sg:person.01270102723.28
    103 rdf:rest Na770fcbd37c549dda33f32603083bd6b
    104 Nacb6dbf11f3b4799967be9bee4ad2b01 schema:name pubmed_id
    105 schema:value 23535868
    106 rdf:type schema:PropertyValue
    107 Nb615a5644a3b44d296ca06f2d4edc941 rdf:first sg:person.01363624355.34
    108 rdf:rest Na90276c314cf41a9969870716617536a
    109 Nbdabbaa3baa64299896e4b61aebc1e27 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Plant Roots
    111 rdf:type schema:DefinedTerm
    112 Nc9c72b8047f841c1b8741766af0d3041 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Coleoptera
    114 rdf:type schema:DefinedTerm
    115 Nd1393a9a3b1b455b8ff1971083f7bfdf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Recombinant Proteins
    117 rdf:type schema:DefinedTerm
    118 Nd801576fefc84b00803e7a8129e9f312 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Matrix Attachment Regions
    120 rdf:type schema:DefinedTerm
    121 Nd8a291e7603142babbecdefe0d522131 rdf:first Neb7eeb6284cf45f788d7033a887b0d4f
    122 rdf:rest N79ad736448b24be9af2a46d1549de01d
    123 Ndb97a6cfa2c7449fa97a55df36423c66 rdf:first sg:person.0743360071.96
    124 rdf:rest Nd8a291e7603142babbecdefe0d522131
    125 Ne29ac12b196948e8aecf1d73407dd333 schema:issueNumber 8
    126 rdf:type schema:PublicationIssue
    127 Ne6479f1215494d48b310947bb9bbb04d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    128 schema:name Transcription, Genetic
    129 rdf:type schema:DefinedTerm
    130 Neb7eeb6284cf45f788d7033a887b0d4f schema:affiliation https://www.grid.ac/institutes/grid.464356.6
    131 schema:familyName Chi
    132 schema:givenName Jing
    133 rdf:type schema:Person
    134 Nff2db9922aa24a48b36467867270feaa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    135 schema:name Animals
    136 rdf:type schema:DefinedTerm
    137 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    138 schema:name Biological Sciences
    139 rdf:type schema:DefinedTerm
    140 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    141 schema:name Genetics
    142 rdf:type schema:DefinedTerm
    143 sg:journal.1124809 schema:issn 0721-7714
    144 1432-203X
    145 schema:name Plant Cell Reports
    146 rdf:type schema:Periodical
    147 sg:person.01105541123.50 schema:affiliation https://www.grid.ac/institutes/grid.464356.6
    148 schema:familyName Zhang
    149 schema:givenName Jie
    150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105541123.50
    151 rdf:type schema:Person
    152 sg:person.01270102723.28 schema:affiliation https://www.grid.ac/institutes/grid.464356.6
    153 schema:familyName Song
    154 schema:givenName Fuping
    155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270102723.28
    156 rdf:type schema:Person
    157 sg:person.01363624355.34 schema:affiliation https://www.grid.ac/institutes/grid.1003.2
    158 schema:familyName Gresshoff
    159 schema:givenName Peter M.
    160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363624355.34
    161 rdf:type schema:Person
    162 sg:person.0662453611.56 schema:affiliation https://www.grid.ac/institutes/grid.464356.6
    163 schema:familyName Shu
    164 schema:givenName Changlong
    165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662453611.56
    166 rdf:type schema:Person
    167 sg:person.0727054501.27 schema:affiliation https://www.grid.ac/institutes/grid.418873.1
    168 schema:familyName Huang
    169 schema:givenName Dafang
    170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727054501.27
    171 rdf:type schema:Person
    172 sg:person.0743360071.96 schema:affiliation https://www.grid.ac/institutes/grid.464356.6
    173 schema:familyName Geng
    174 schema:givenName Lili
    175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0743360071.96
    176 rdf:type schema:Person
    177 sg:pub.10.1007/978-90-481-2245-5_1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033829161
    178 https://doi.org/10.1007/978-90-481-2245-5_1
    179 rdf:type schema:CreativeWork
    180 sg:pub.10.1007/978-94-007-2217-0_1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049764614
    181 https://doi.org/10.1007/978-94-007-2217-0_1
    182 rdf:type schema:CreativeWork
    183 sg:pub.10.1007/bf00010152 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014293696
    184 https://doi.org/10.1007/bf00010152
    185 rdf:type schema:CreativeWork
    186 sg:pub.10.1007/s00253-007-1040-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009029709
    187 https://doi.org/10.1007/s00253-007-1040-7
    188 rdf:type schema:CreativeWork
    189 sg:pub.10.1007/s00253-010-2479-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043836914
    190 https://doi.org/10.1007/s00253-010-2479-5
    191 rdf:type schema:CreativeWork
    192 sg:pub.10.1007/s00284-008-9338-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1015190150
    193 https://doi.org/10.1007/s00284-008-9338-y
    194 rdf:type schema:CreativeWork
    195 sg:pub.10.1007/s00299-008-0525-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004357149
    196 https://doi.org/10.1007/s00299-008-0525-x
    197 rdf:type schema:CreativeWork
    198 sg:pub.10.1007/s00299-011-1167-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1042252900
    199 https://doi.org/10.1007/s00299-011-1167-y
    200 rdf:type schema:CreativeWork
    201 sg:pub.10.1007/s11240-012-0113-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049226773
    202 https://doi.org/10.1007/s11240-012-0113-1
    203 rdf:type schema:CreativeWork
    204 sg:pub.10.1007/s11248-004-5413-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048508160
    205 https://doi.org/10.1007/s11248-004-5413-8
    206 rdf:type schema:CreativeWork
    207 sg:pub.10.1007/s11248-008-9230-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049252548
    208 https://doi.org/10.1007/s11248-008-9230-3
    209 rdf:type schema:CreativeWork
    210 sg:pub.10.1007/s12355-011-0080-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035485224
    211 https://doi.org/10.1007/s12355-011-0080-6
    212 rdf:type schema:CreativeWork
    213 sg:pub.10.1023/a:1018481805928 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045821799
    214 https://doi.org/10.1023/a:1018481805928
    215 rdf:type schema:CreativeWork
    216 sg:pub.10.1038/nprot.2007.141 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021137406
    217 https://doi.org/10.1038/nprot.2007.141
    218 rdf:type schema:CreativeWork
    219 https://doi.org/10.1002/ps.1701 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032867062
    220 rdf:type schema:CreativeWork
    221 https://doi.org/10.1002/ps.2041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041483727
    222 rdf:type schema:CreativeWork
    223 https://doi.org/10.1016/j.jip.2007.09.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053348623
    224 rdf:type schema:CreativeWork
    225 https://doi.org/10.1016/s0261-2194(99)00028-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037915963
    226 rdf:type schema:CreativeWork
    227 https://doi.org/10.1016/s1360-1385(98)01198-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037482240
    228 rdf:type schema:CreativeWork
    229 https://doi.org/10.1080/09583157.2011.566324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013208090
    230 rdf:type schema:CreativeWork
    231 https://doi.org/10.1080/09670870500399866 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005600480
    232 rdf:type schema:CreativeWork
    233 https://doi.org/10.1093/jee/100.2.431 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013848664
    234 rdf:type schema:CreativeWork
    235 https://doi.org/10.1093/treephys/28.1.55 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025218609
    236 rdf:type schema:CreativeWork
    237 https://doi.org/10.1104/pp.107.111096 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012493034
    238 rdf:type schema:CreativeWork
    239 https://doi.org/10.1111/j.1365-313x.2010.04398.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1001982211
    240 rdf:type schema:CreativeWork
    241 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023703429
    242 rdf:type schema:CreativeWork
    243 https://doi.org/10.1128/aem.01074-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049709351
    244 rdf:type schema:CreativeWork
    245 https://doi.org/10.1128/mcb.10.5.2302 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017428371
    246 rdf:type schema:CreativeWork
    247 https://doi.org/10.1603/0022-0493(2007)100[431:reoioj]2.0.co;2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041206754
    248 rdf:type schema:CreativeWork
    249 https://www.grid.ac/institutes/grid.1003.2 schema:alternateName University of Queensland
    250 schema:name ARC Centre of Excellence for Integrative Legume Research, The University of Queensland, St. Lucia, 4072, Brisbane, QLD, Australia
    251 rdf:type schema:Organization
    252 https://www.grid.ac/institutes/grid.418873.1 schema:alternateName Biotechnology Research Institute
    253 schema:name Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, China
    254 rdf:type schema:Organization
    255 https://www.grid.ac/institutes/grid.464356.6 schema:alternateName Institute of Plant Protection
    256 schema:name State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China
    257 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...