Expression of a glycosylated GFP as a bivalent reporter in exocytosis View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-01

AUTHORS

Nadine Paris, Bruno Saint-Jean, Marianna Faraco, Weronika Krzeszowiec, Giuseppe Dalessandro, Jean-Marc Neuhaus, Gian Pietro Di Sansebastiano

ABSTRACT

The complex-type N-linked glycans of plants differ markedly in structure from those of animals. Like those of insects and mollusks they lack terminal sialic acid(s) and may contain an alpha-(1,3)-fucose (Fuc) linked to the proximal GlcNAc residue and/or a beta-(1,2)-xylose (Xyl) residue attached to the proximal mannose (Man) of the glycan core. N-glycosylated GFPs were used in previous studies showing their effective use to report on membrane traffic between the ER and the Golgi apparatus in plant cells. In all these cases glycosylated tags were added at the GFP termini. Because of the position of the tag and depending on the sorting and accumulation site of these modified GFP, there is always a risk of processing and degradation, and this protein design cannot be considered ideal. Here, we describe the development of three different GFPs in which the glycosylation site is internally localized at positions 80, 133, or 172 in the internal sequence. The best glycosylation site was at position 133. This glycosylated GFPgl133 appears to be protected from undesired processing of the glycosylation site and represents a bivalent reporter for biochemical and microscopic studies. After experimental validation, we can conclude that amino acid 133 is an effective glycosylation site and that the GFPgl133 is a powerful tool for in vivo investigations in plant cell biology. More... »

PAGES

79

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00299-009-0799-7

DOI

http://dx.doi.org/10.1007/s00299-009-0799-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002412201

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19957086


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Exocytosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Reporter", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glycosylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Golgi Apparatus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Green Fluorescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Confocal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protoplasts", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tobacco", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Biochemistry and Plant Molecular Physiology", 
          "id": "https://www.grid.ac/institutes/grid.461861.c", 
          "name": [
            "INRA, Biochemistry and Plant Molecular Physiology, IBIP, B\u00e2t. 7, 2 place Pierre Viala, 34060, Montpellier, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Paris", 
        "givenName": "Nadine", 
        "id": "sg:person.0764164646.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764164646.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "French Research Institute for Exploitation of the Sea", 
          "id": "https://www.grid.ac/institutes/grid.4825.b", 
          "name": [
            "Laboratoire de Physiologie et Biotechnologie des Algues, IFREMER, Rue de l\u2019Ile d\u2019Yeu, BP21105, 44311, Nantes Cedex 03, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Saint-Jean", 
        "givenName": "Bruno", 
        "id": "sg:person.0675400375.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675400375.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Salento", 
          "id": "https://www.grid.ac/institutes/grid.9906.6", 
          "name": [
            "Di.S.Te.B.A., Universit\u00e0 del Salento, via prov. Lecce-Monteroni, 73100, Lecce, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Faraco", 
        "givenName": "Marianna", 
        "id": "sg:person.01242433563.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242433563.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jagiellonian University", 
          "id": "https://www.grid.ac/institutes/grid.5522.0", 
          "name": [
            "Di.S.Te.B.A., Universit\u00e0 del Salento, via prov. Lecce-Monteroni, 73100, Lecce, Italy", 
            "Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa Str.7, 30-387, Krak\u00f3w, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Krzeszowiec", 
        "givenName": "Weronika", 
        "id": "sg:person.01101642751.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101642751.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Salento", 
          "id": "https://www.grid.ac/institutes/grid.9906.6", 
          "name": [
            "Di.S.Te.B.A., Universit\u00e0 del Salento, via prov. Lecce-Monteroni, 73100, Lecce, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dalessandro", 
        "givenName": "Giuseppe", 
        "id": "sg:person.0647102741.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647102741.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Neuch\u00e2tel", 
          "id": "https://www.grid.ac/institutes/grid.10711.36", 
          "name": [
            "Laboratory of Cell and Molecular Biology, University of Neuch\u00e2tel, Rue Emile-Argand 11, CP 158, 2009, Neuch\u00e2tel, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Neuhaus", 
        "givenName": "Jean-Marc", 
        "id": "sg:person.0631527437.73", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0631527437.73"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Salento", 
          "id": "https://www.grid.ac/institutes/grid.9906.6", 
          "name": [
            "Di.S.Te.B.A., Universit\u00e0 del Salento, via prov. Lecce-Monteroni, 73100, Lecce, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Di Sansebastiano", 
        "givenName": "Gian Pietro", 
        "id": "sg:person.01121052614.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01121052614.18"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s10535-007-0010-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001220249", 
          "https://doi.org/10.1007/s10535-007-0010-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0304-4165(99)00181-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009633663"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tibtech.2007.04.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010741793"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/11263500410001684107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012464081"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-4889(98)00061-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013386932"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.2.4.301", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017393318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.000620", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018849966"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.104.026351", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020112880"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0708236104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021068880"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.105.036400", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023560111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/11263500802150779", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025308929"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/pcp/pcn051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026333912"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00709-005-0139-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028092175", 
          "https://doi.org/10.1007/s00709-005-0139-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00709-005-0139-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028092175", 
          "https://doi.org/10.1007/s00709-005-0139-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200407072", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030825845"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.pp.46.060195.001401", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033584623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1998.00210.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035140833"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1998.00210.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035140833"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.105.040394", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035429959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/26.2.623", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045791827"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00425-006-0407-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046837877", 
          "https://doi.org/10.1007/s00425-006-0407-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00425-006-0407-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046837877", 
          "https://doi.org/10.1007/s00425-006-0407-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1006012005654", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047252811", 
          "https://doi.org/10.1023/a:1006012005654"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-0348-8876-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048794569", 
          "https://doi.org/10.1007/978-3-0348-8876-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-0348-8876-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048794569", 
          "https://doi.org/10.1007/978-3-0348-8876-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1467-7652.2006.00200.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049105010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.12.11.2201", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052456168"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3869078", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070467158"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3871115", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070468928"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/psb.1.2.2621", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072307346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075032411", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2010-01", 
    "datePublishedReg": "2010-01-01", 
    "description": "The complex-type N-linked glycans of plants differ markedly in structure from those of animals. Like those of insects and mollusks they lack terminal sialic acid(s) and may contain an alpha-(1,3)-fucose (Fuc) linked to the proximal GlcNAc residue and/or a beta-(1,2)-xylose (Xyl) residue attached to the proximal mannose (Man) of the glycan core. N-glycosylated GFPs were used in previous studies showing their effective use to report on membrane traffic between the ER and the Golgi apparatus in plant cells. In all these cases glycosylated tags were added at the GFP termini. Because of the position of the tag and depending on the sorting and accumulation site of these modified GFP, there is always a risk of processing and degradation, and this protein design cannot be considered ideal. Here, we describe the development of three different GFPs in which the glycosylation site is internally localized at positions 80, 133, or 172 in the internal sequence. The best glycosylation site was at position 133. This glycosylated GFPgl133 appears to be protected from undesired processing of the glycosylation site and represents a bivalent reporter for biochemical and microscopic studies. After experimental validation, we can conclude that amino acid 133 is an effective glycosylation site and that the GFPgl133 is a powerful tool for in vivo investigations in plant cell biology.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00299-009-0799-7", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.5203182", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1124809", 
        "issn": [
          "0721-7714", 
          "1432-203X"
        ], 
        "name": "Plant Cell Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "29"
      }
    ], 
    "name": "Expression of a glycosylated GFP as a bivalent reporter in exocytosis", 
    "pagination": "79", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "0b7c021f9ce8b04c1da389b58c58867cef45c94c650093dfdf4ace0c50ff5103"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19957086"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9880970"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00299-009-0799-7"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1002412201"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00299-009-0799-7", 
      "https://app.dimensions.ai/details/publication/pub.1002412201"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T14:33", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000373_0000000373/records_13106_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs00299-009-0799-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00299-009-0799-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00299-009-0799-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00299-009-0799-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00299-009-0799-7'


 

This table displays all metadata directly associated to this object as RDF triples.

247 TRIPLES      21 PREDICATES      65 URIs      30 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00299-009-0799-7 schema:about N074aa1c00abc4b9c8424b617cdc087c7
2 N240dbd6798d24d78a3aa0a74c80329b8
3 N2a37363ff2bc41d8942f30e891f87f72
4 N2d3b9c7a332c4ed78922945139fcc7b4
5 N47f89bca0556479a900aaffa44248390
6 N975eb5fa0bb24890808849a0632e6a39
7 Nc5ab0c6af23a4a648842840e79f48a35
8 Ndf5d477fafdf4fbea27927a8023fdb5e
9 Ne2427dde70f34b3ca9054c3c402e47f2
10 anzsrc-for:06
11 anzsrc-for:0601
12 schema:author N8261248c73454324bd587075dff090f9
13 schema:citation sg:pub.10.1007/978-3-0348-8876-9
14 sg:pub.10.1007/s00425-006-0407-9
15 sg:pub.10.1007/s00709-005-0139-x
16 sg:pub.10.1007/s10535-007-0010-3
17 sg:pub.10.1023/a:1006012005654
18 https://app.dimensions.ai/details/publication/pub.1075032411
19 https://doi.org/10.1016/j.tibtech.2007.04.008
20 https://doi.org/10.1016/s0167-4889(98)00061-5
21 https://doi.org/10.1016/s0304-4165(99)00181-6
22 https://doi.org/10.1046/j.1365-313x.1998.00210.x
23 https://doi.org/10.1073/pnas.0708236104
24 https://doi.org/10.1080/11263500410001684107
25 https://doi.org/10.1080/11263500802150779
26 https://doi.org/10.1083/jcb.200407072
27 https://doi.org/10.1093/nar/26.2.623
28 https://doi.org/10.1093/pcp/pcn051
29 https://doi.org/10.1105/tpc.000620
30 https://doi.org/10.1105/tpc.104.026351
31 https://doi.org/10.1105/tpc.105.036400
32 https://doi.org/10.1105/tpc.105.040394
33 https://doi.org/10.1105/tpc.12.11.2201
34 https://doi.org/10.1105/tpc.2.4.301
35 https://doi.org/10.1111/j.1467-7652.2006.00200.x
36 https://doi.org/10.1146/annurev.pp.46.060195.001401
37 https://doi.org/10.2307/3869078
38 https://doi.org/10.2307/3871115
39 https://doi.org/10.4161/psb.1.2.2621
40 schema:datePublished 2010-01
41 schema:datePublishedReg 2010-01-01
42 schema:description The complex-type N-linked glycans of plants differ markedly in structure from those of animals. Like those of insects and mollusks they lack terminal sialic acid(s) and may contain an alpha-(1,3)-fucose (Fuc) linked to the proximal GlcNAc residue and/or a beta-(1,2)-xylose (Xyl) residue attached to the proximal mannose (Man) of the glycan core. N-glycosylated GFPs were used in previous studies showing their effective use to report on membrane traffic between the ER and the Golgi apparatus in plant cells. In all these cases glycosylated tags were added at the GFP termini. Because of the position of the tag and depending on the sorting and accumulation site of these modified GFP, there is always a risk of processing and degradation, and this protein design cannot be considered ideal. Here, we describe the development of three different GFPs in which the glycosylation site is internally localized at positions 80, 133, or 172 in the internal sequence. The best glycosylation site was at position 133. This glycosylated GFPgl133 appears to be protected from undesired processing of the glycosylation site and represents a bivalent reporter for biochemical and microscopic studies. After experimental validation, we can conclude that amino acid 133 is an effective glycosylation site and that the GFPgl133 is a powerful tool for in vivo investigations in plant cell biology.
43 schema:genre research_article
44 schema:inLanguage en
45 schema:isAccessibleForFree true
46 schema:isPartOf N6aaa20a6e7744d2c862d5533ca831152
47 Nfe30a1dd056a4335bf18d4afbdb97f65
48 sg:journal.1124809
49 schema:name Expression of a glycosylated GFP as a bivalent reporter in exocytosis
50 schema:pagination 79
51 schema:productId N13f3d3e01189475ebf1e94b179963a92
52 N48a801e768514fb38c357c52b4f3b624
53 N5d0cbb18b67c49fdbd81674766038af0
54 N728147a70962409ea26542c2e11dcb88
55 Nacb504932de3440ba7216b5e2a0bdbfa
56 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002412201
57 https://doi.org/10.1007/s00299-009-0799-7
58 schema:sdDatePublished 2019-04-11T14:33
59 schema:sdLicense https://scigraph.springernature.com/explorer/license/
60 schema:sdPublisher Nf66cf8a964dd48ce8950ed1668027dee
61 schema:url https://link.springer.com/10.1007%2Fs00299-009-0799-7
62 sgo:license sg:explorer/license/
63 sgo:sdDataset articles
64 rdf:type schema:ScholarlyArticle
65 N074aa1c00abc4b9c8424b617cdc087c7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
66 schema:name Glycosylation
67 rdf:type schema:DefinedTerm
68 N13f3d3e01189475ebf1e94b179963a92 schema:name pubmed_id
69 schema:value 19957086
70 rdf:type schema:PropertyValue
71 N240dbd6798d24d78a3aa0a74c80329b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Protoplasts
73 rdf:type schema:DefinedTerm
74 N25c26e09721640b2b81309300ed30188 rdf:first sg:person.0675400375.17
75 rdf:rest N79a1e6a6ba654c35b27fc5ec7be3f803
76 N266fc561774e4e11bbaac621fe3434c1 rdf:first sg:person.0631527437.73
77 rdf:rest N3c6846c1c8034939ab3191b7cdb66a26
78 N2a37363ff2bc41d8942f30e891f87f72 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Exocytosis
80 rdf:type schema:DefinedTerm
81 N2d3b9c7a332c4ed78922945139fcc7b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Tobacco
83 rdf:type schema:DefinedTerm
84 N344da446cdaa4fc8a6c1113b2a52de3f rdf:first sg:person.01101642751.53
85 rdf:rest Ne6f8a75c025343e3ab5b3d05a3a2ce53
86 N3c6846c1c8034939ab3191b7cdb66a26 rdf:first sg:person.01121052614.18
87 rdf:rest rdf:nil
88 N47f89bca0556479a900aaffa44248390 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Plant Proteins
90 rdf:type schema:DefinedTerm
91 N48a801e768514fb38c357c52b4f3b624 schema:name nlm_unique_id
92 schema:value 9880970
93 rdf:type schema:PropertyValue
94 N5d0cbb18b67c49fdbd81674766038af0 schema:name doi
95 schema:value 10.1007/s00299-009-0799-7
96 rdf:type schema:PropertyValue
97 N6aaa20a6e7744d2c862d5533ca831152 schema:issueNumber 1
98 rdf:type schema:PublicationIssue
99 N728147a70962409ea26542c2e11dcb88 schema:name dimensions_id
100 schema:value pub.1002412201
101 rdf:type schema:PropertyValue
102 N79a1e6a6ba654c35b27fc5ec7be3f803 rdf:first sg:person.01242433563.52
103 rdf:rest N344da446cdaa4fc8a6c1113b2a52de3f
104 N8261248c73454324bd587075dff090f9 rdf:first sg:person.0764164646.15
105 rdf:rest N25c26e09721640b2b81309300ed30188
106 N975eb5fa0bb24890808849a0632e6a39 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Microscopy, Confocal
108 rdf:type schema:DefinedTerm
109 Nacb504932de3440ba7216b5e2a0bdbfa schema:name readcube_id
110 schema:value 0b7c021f9ce8b04c1da389b58c58867cef45c94c650093dfdf4ace0c50ff5103
111 rdf:type schema:PropertyValue
112 Nc5ab0c6af23a4a648842840e79f48a35 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Green Fluorescent Proteins
114 rdf:type schema:DefinedTerm
115 Ndf5d477fafdf4fbea27927a8023fdb5e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Golgi Apparatus
117 rdf:type schema:DefinedTerm
118 Ne2427dde70f34b3ca9054c3c402e47f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Genes, Reporter
120 rdf:type schema:DefinedTerm
121 Ne6f8a75c025343e3ab5b3d05a3a2ce53 rdf:first sg:person.0647102741.37
122 rdf:rest N266fc561774e4e11bbaac621fe3434c1
123 Nf66cf8a964dd48ce8950ed1668027dee schema:name Springer Nature - SN SciGraph project
124 rdf:type schema:Organization
125 Nfe30a1dd056a4335bf18d4afbdb97f65 schema:volumeNumber 29
126 rdf:type schema:PublicationVolume
127 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
128 schema:name Biological Sciences
129 rdf:type schema:DefinedTerm
130 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
131 schema:name Biochemistry and Cell Biology
132 rdf:type schema:DefinedTerm
133 sg:grant.5203182 http://pending.schema.org/fundedItem sg:pub.10.1007/s00299-009-0799-7
134 rdf:type schema:MonetaryGrant
135 sg:journal.1124809 schema:issn 0721-7714
136 1432-203X
137 schema:name Plant Cell Reports
138 rdf:type schema:Periodical
139 sg:person.01101642751.53 schema:affiliation https://www.grid.ac/institutes/grid.5522.0
140 schema:familyName Krzeszowiec
141 schema:givenName Weronika
142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101642751.53
143 rdf:type schema:Person
144 sg:person.01121052614.18 schema:affiliation https://www.grid.ac/institutes/grid.9906.6
145 schema:familyName Di Sansebastiano
146 schema:givenName Gian Pietro
147 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01121052614.18
148 rdf:type schema:Person
149 sg:person.01242433563.52 schema:affiliation https://www.grid.ac/institutes/grid.9906.6
150 schema:familyName Faraco
151 schema:givenName Marianna
152 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242433563.52
153 rdf:type schema:Person
154 sg:person.0631527437.73 schema:affiliation https://www.grid.ac/institutes/grid.10711.36
155 schema:familyName Neuhaus
156 schema:givenName Jean-Marc
157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0631527437.73
158 rdf:type schema:Person
159 sg:person.0647102741.37 schema:affiliation https://www.grid.ac/institutes/grid.9906.6
160 schema:familyName Dalessandro
161 schema:givenName Giuseppe
162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647102741.37
163 rdf:type schema:Person
164 sg:person.0675400375.17 schema:affiliation https://www.grid.ac/institutes/grid.4825.b
165 schema:familyName Saint-Jean
166 schema:givenName Bruno
167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675400375.17
168 rdf:type schema:Person
169 sg:person.0764164646.15 schema:affiliation https://www.grid.ac/institutes/grid.461861.c
170 schema:familyName Paris
171 schema:givenName Nadine
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764164646.15
173 rdf:type schema:Person
174 sg:pub.10.1007/978-3-0348-8876-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048794569
175 https://doi.org/10.1007/978-3-0348-8876-9
176 rdf:type schema:CreativeWork
177 sg:pub.10.1007/s00425-006-0407-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046837877
178 https://doi.org/10.1007/s00425-006-0407-9
179 rdf:type schema:CreativeWork
180 sg:pub.10.1007/s00709-005-0139-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1028092175
181 https://doi.org/10.1007/s00709-005-0139-x
182 rdf:type schema:CreativeWork
183 sg:pub.10.1007/s10535-007-0010-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001220249
184 https://doi.org/10.1007/s10535-007-0010-3
185 rdf:type schema:CreativeWork
186 sg:pub.10.1023/a:1006012005654 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047252811
187 https://doi.org/10.1023/a:1006012005654
188 rdf:type schema:CreativeWork
189 https://app.dimensions.ai/details/publication/pub.1075032411 schema:CreativeWork
190 https://doi.org/10.1016/j.tibtech.2007.04.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010741793
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/s0167-4889(98)00061-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013386932
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/s0304-4165(99)00181-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009633663
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1046/j.1365-313x.1998.00210.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1035140833
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1073/pnas.0708236104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021068880
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1080/11263500410001684107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012464081
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1080/11263500802150779 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025308929
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1083/jcb.200407072 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030825845
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1093/nar/26.2.623 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045791827
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1093/pcp/pcn051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026333912
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1105/tpc.000620 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018849966
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1105/tpc.104.026351 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020112880
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1105/tpc.105.036400 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023560111
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1105/tpc.105.040394 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035429959
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1105/tpc.12.11.2201 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052456168
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1105/tpc.2.4.301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017393318
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1111/j.1467-7652.2006.00200.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1049105010
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1146/annurev.pp.46.060195.001401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033584623
225 rdf:type schema:CreativeWork
226 https://doi.org/10.2307/3869078 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070467158
227 rdf:type schema:CreativeWork
228 https://doi.org/10.2307/3871115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070468928
229 rdf:type schema:CreativeWork
230 https://doi.org/10.4161/psb.1.2.2621 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072307346
231 rdf:type schema:CreativeWork
232 https://www.grid.ac/institutes/grid.10711.36 schema:alternateName University of Neuchâtel
233 schema:name Laboratory of Cell and Molecular Biology, University of Neuchâtel, Rue Emile-Argand 11, CP 158, 2009, Neuchâtel, Switzerland
234 rdf:type schema:Organization
235 https://www.grid.ac/institutes/grid.461861.c schema:alternateName Biochemistry and Plant Molecular Physiology
236 schema:name INRA, Biochemistry and Plant Molecular Physiology, IBIP, Bât. 7, 2 place Pierre Viala, 34060, Montpellier, France
237 rdf:type schema:Organization
238 https://www.grid.ac/institutes/grid.4825.b schema:alternateName French Research Institute for Exploitation of the Sea
239 schema:name Laboratoire de Physiologie et Biotechnologie des Algues, IFREMER, Rue de l’Ile d’Yeu, BP21105, 44311, Nantes Cedex 03, France
240 rdf:type schema:Organization
241 https://www.grid.ac/institutes/grid.5522.0 schema:alternateName Jagiellonian University
242 schema:name Di.S.Te.B.A., Università del Salento, via prov. Lecce-Monteroni, 73100, Lecce, Italy
243 Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa Str.7, 30-387, Kraków, Poland
244 rdf:type schema:Organization
245 https://www.grid.ac/institutes/grid.9906.6 schema:alternateName University of Salento
246 schema:name Di.S.Te.B.A., Università del Salento, via prov. Lecce-Monteroni, 73100, Lecce, Italy
247 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...