Transformation of Kalanchoe blossfeldiana with rol-genes is useful in molecular breeding towards compact growth View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-09

AUTHORS

Brian Christensen, Sridevy Sriskandarajah, Margrethe Serek, Renate Müller

ABSTRACT

Dwarf genotypes of the economically important flowering potted plant Kalanchoe blossfeldiana were developed by molecular breeding. Root inducing (Ri)-lines were regenerated by applying CPPU to the hairy roots, which were produced by inoculating leaf explants with a wild-type Agrobacterium rhizogenes strain ATCC15834. Amplification by polymerase chain reaction (PCR) and Southern blot analysis confirmed the presence of T-DNA in the Ri-lines. Six Ri-lines were characterised in a greenhouse trial revealing that several morphological traits changed with respect to ornamental value such as plant height, number of lateral shoots, leaf size, leaf number, flower size and number of flowers. The Ri-lines differed in their degree of Ri-phenotype, and the internodes of the Ri-lines were clearly shorter, giving a compact growth habit compared to control plants. Time to anthesis was the same in Ri-line 331 as in control plants and delayed by only 3 days in Ri-line 306 as compared to control plants. A compact plant without delayed flowering can be assumed to be valuable for further breeding. More... »

PAGES

1485-1495

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00299-008-0575-0

DOI

http://dx.doi.org/10.1007/s00299-008-0575-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030671166

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18597094


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