Agrobacterium-mediated transformation of the dwarf pomegranate (Punica granatum L. var. nana) View Full Text


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Article Info

DATE

2007-04-24

AUTHORS

Shingo Terakami, Nagao Matsuta, Toshiya Yamamoto, Sumiko Sugaya, Hiroshi Gemma, Junichi Soejima

ABSTRACT

The dwarf pomegranate (Punica granatum L. var. nana) is a dwarf ornamental plant that has the potential to be the model plant of perennial fruit trees because it bears fruits within 1 year of seedling. We established an Agrobacterium-mediated transformation system for the dwarf pomegranate. Adventitious shoots regenerated from leaf segments were inoculated with A. tumefaciens strain EHA105 harboring the binary vector pBin19-sgfp, which contains neomycin phosphotransferase (npt II) and green fluorescent protein (gfp) gene as a selectable and visual marker, respectively. After co-cultivation, the inoculated adventitious shoots were cut into small pieces to induce regeneration, and then selected on MS medium supplemented with 0.5 μM α-naphthaleneacetic acid (NAA), 5 μM N6-benzyladenine (BA), 0.3% gellan gum, 50 mg/l kanamycin, and 10 mg/l meropenem. Putative transformed shoots were regenerated after 6–8 months of selection. PCR and PCR-Southern blot analysis revealed the integration of the transgene into the plant genome. Transformants bloomed and bore fruits within 3 months of being potted, and the inheritance of the transgene was confirmed in T1 generations. The advantage of the transformation of dwarf pomegranate was shown to be the high transformation rate. The establishment of this transformation system is invaluable for investigating fruit-tree-specific phenomena. More... »

PAGES

1243-1251

References to SciGraph publications

  • 2001-06-01. Agrobacterium-mediated transformation of Campanula glomerata in PLANT CELL REPORTS
  • 2003-12. In vitro induction of tetraploid in pomegranate (Punica granatum) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2004-08-05. Agrobacterium tumefaciens-mediated transformation of blueberry (Vaccinium corymbosum L.) in PLANT CELL REPORTS
  • 1993-07. NewAgrobacterium helper plasmids for gene transfer to plants in TRANSGENIC RESEARCH
  • 1999-01. Transgenic almond (Prunus dulcis Mill.) plants obtained by Agrobacterium-mediated transformation of leaf explants in PLANT CELL REPORTS
  • 2004-05-05. Agrobacterium-mediated genetic transformation and development of herbicide-resistant sugarcane (Saccharum species hybrids) using axillary buds in PLANT CELL REPORTS
  • 1999-07. Green fluorescent protein as a screenable marker to increase the efficiency of generating transgenic woody fruit plants in THEORETICAL AND APPLIED GENETICS
  • 1996-03. Transformation of 12 different plasmids into soybean via particle bombardment in PLANT CELL REPORTS
  • 2002-06-01. Appropriate choice of antibiotic and Agrobacterium strain improves transformation of antibiotic-sensitive Fragaria vesca and F. v. semperflorens in PLANT CELL REPORTS
  • 2005-02. Transformation of fruit trees. Useful breeding tool or continued future prospect? in TRANSGENIC RESEARCH
  • 1983-05. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid in NATURE
  • 2003-05-27. Ethylene inhibitors and low kanamycin concentrations improve adventitious regeneration from apricot leaves in PLANT CELL REPORTS
  • 2000-01. Influence of the antibiotic timentin on plant regeneration of tomato (Lycopersicon esculentum Mill.) cultivars in PLANT CELL REPORTS
  • 2003-11. Effects of Antibiotics and Bialaphos on the Growth and Development of Embryogenic Callus Cultures of Muscari armeniacum in BIOLOGIA PLANTARUM
  • 1999-11. Agrobacterium tumefaciens-mediated transformation of Pinus pinea L. cotyledons: an assessment of factors influencing the efficiency of uidA gene transfer in PLANT CELL REPORTS
  • 1995-04. Regeneration of transgenic plants from the commercial apple cultivar Royal Gala in PLANT CELL REPORTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00299-007-0347-2

    DOI

    http://dx.doi.org/10.1007/s00299-007-0347-2

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1031813055

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17453216


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