Stable Agrobacterium-mediated genetic transformation of London plane tree (Platanus acerifolia Willd.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-05

AUTHORS

Z. N. Li, F. Fang, G. F. Liu, M. Z. Bao

ABSTRACT

London plane tree (Platanus acerifolia Willd.) is an important tree in urban landscaping but it suffers from a number of negative traits which genetic engineering could be used to address. As with many woody species, P. acerifolia has appeared recalcitrant to genetic transformation. However, the recent development of a method for regenerating shoots from P. acerifolia leaf explants suggests that such material could be a target for gene-transfer. Using an Agrobacterium tumefaciens strain in which the T-DNA carries the histochemically detected reporter gene beta-glucuronidase (GUS), we have followed the transfer of genes from Agrobacterium to leaf explants of Platanus acerifolia. Using this system, we have identified a set of inoculation and co-cultivation conditions (notably: the pre-treatment of leaf explants with 0.4 M mannitol, an inoculation period of 10 min, a bacterial OD(600) of 0.8-1.0 and a co-cultivation period of 5 days) that permit a good frequency and reliability of transient gene-transfer. Optimum levels of antibiotics for bacterial elimination and kanamycin-resistant shoot regeneration were also established. By applying these parameters, we recovered eight independent stably transformed shoots that were kanamycin-resistant and contained the nptII T-DNA gene, as confirmed by PCR analysis. Furthermore, Southern blot analysis confirmed that, in at least five of these lines, the transgene was associated with high molecular weight DNA, so indicating integration into the plant genome. More... »

PAGES

641-650

References to SciGraph publications

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  • 2002-11. Plant regeneration from excised hypocotyl explants of Platanus acerifolia willd in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 2002-02. Agrobacterium-mediated production of transgenic plants of Muscari armeniacum Leichtl. ex Bak. in PLANT CELL REPORTS
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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00299-006-0271-x

    DOI

    http://dx.doi.org/10.1007/s00299-006-0271-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1022798548

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17165043


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