Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-01

AUTHORS

Alida Ballester, Magdalena Cervera, Leandro Peña

ABSTRACT

The presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. In citrus, selection using the selectable marker gene nptII, that confers resistance to the antibiotic kanamycin, is in general very effective. An attractive alternative is offered by the MAT system (Multi-Auto-Transformation), which combines the ipt gene for positive selection with the recombinase system R/RS for removal of marker genes from transgenic cells after transformation. Transformation with a MAT vector has been attempted in two citrus genotypes, Pineapple sweet orange (Citrus sinensis L. Osb.) and Carrizo citrange (C. sinensis L. Osb. x Poncirus trifoliata L. Raf.). Results indicated that the IPT phenotype was clearly distinguishable in sweet orange but not in citrange, and that excision was not always efficient and precise. Nevertheless, the easy visual detection of the IPT phenotype combined with the higher transformation efficiency achieved in sweet orange using this system open interesting perspectives for the generation of marker-free transgenic citrus plants. More... »

PAGES

39-45

References to SciGraph publications

  • 1998-12. Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration in PLANT CELL REPORTS
  • 1999-07. Green fluorescent protein as a screenable marker to increase the efficiency of generating transgenic woody fruit plants in THEORETICAL AND APPLIED GENETICS
  • 1983-07. From tumour to tuber; tumour cell characteristics and chromosome numbers of crown gall-derived tetraploid potato plants (Solanum tuberosum cv. ‘Maris Bard’) in THEORETICAL AND APPLIED GENETICS
  • 1998-01. Genetic transformation and regeneration of mature tissues of woody fruit plants bypassing the juvenile stage in TRANSGENIC RESEARCH
  • 2002-09. Asexual production of selectable marker-free transgenic woody plants, vegetatively propagated species in MOLECULAR BREEDING
  • 1995-07. Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants in PLANT CELL REPORTS
  • 1999-08. Effective selection system for generating marker-free transgenic plants independent of sexual crossing in PLANT CELL REPORTS
  • 1983-09. A plant DNA minipreparation: Version II in PLANT MOLECULAR BIOLOGY REPORTER
  • 2004. Agrobacterum-Mediated Transformation of Citrus in TRANSGENIC CROPS OF THE WORLD
  • 2004-09. Characterisation of regenerants obtained under selective conditions after Agrobacterium-mediated transformation of citrus explants reveals production of silenced and chimeric plants at unexpected high frequencies in MOLECULAR BREEDING
  • 2006-02. Induction, suppression and requirement of RNA silencing pathways in virulent Agrobacterium tumefaciens infections in NATURE GENETICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00299-006-0197-3

    DOI

    http://dx.doi.org/10.1007/s00299-006-0197-3

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1010968345

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16927091


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Alkyl and Aryl Transferases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Citrus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Citrus sinensis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Markers", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Vectors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plant Shoots", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants, Genetically Modified", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombinases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombination, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transformation, Genetic", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Instituto Valenciano de Investigaciones Agrarias", 
              "id": "https://www.grid.ac/institutes/grid.419276.f", 
              "name": [
                "Department Plant Protection and Biotechnology, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial 46113-Moncada, Valencia, Spain"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ballester", 
            "givenName": "Alida", 
            "id": "sg:person.01263224217.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01263224217.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Instituto Valenciano de Investigaciones Agrarias", 
              "id": "https://www.grid.ac/institutes/grid.419276.f", 
              "name": [
                "Department Plant Protection and Biotechnology, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial 46113-Moncada, Valencia, Spain"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Cervera", 
            "givenName": "Magdalena", 
            "id": "sg:person.01032457452.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01032457452.34"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Instituto Valenciano de Investigaciones Agrarias", 
              "id": "https://www.grid.ac/institutes/grid.419276.f", 
              "name": [
                "Department Plant Protection and Biotechnology, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial 46113-Moncada, Valencia, Spain"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pe\u00f1a", 
            "givenName": "Leandro", 
            "id": "sg:person.0743161043.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0743161043.55"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1093/treephys/20.17.1183", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002652786"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02712670", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003131267", 
              "https://doi.org/10.1007/bf02712670"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02712670", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003131267", 
              "https://doi.org/10.1007/bf02712670"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1020308216747", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004890324", 
              "https://doi.org/10.1023/a:1020308216747"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s002990050570", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007201059", 
              "https://doi.org/10.1007/s002990050570"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s002990050570", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007201059", 
              "https://doi.org/10.1007/s002990050570"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s002990050570", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007201059", 
              "https://doi.org/10.1007/s002990050570"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng1722", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011139424", 
              "https://doi.org/10.1038/ng1722"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng1722", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011139424", 
              "https://doi.org/10.1038/ng1722"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s002990050688", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012967220", 
              "https://doi.org/10.1007/s002990050688"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s002990050688", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012967220", 
              "https://doi.org/10.1007/s002990050688"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s001220051244", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015745462", 
              "https://doi.org/10.1007/s001220051244"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s001220051244", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015745462", 
              "https://doi.org/10.1007/s001220051244"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.2002.01272.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019828039"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00265193", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021579003", 
              "https://doi.org/10.1007/bf00265193"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00265193", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021579003", 
              "https://doi.org/10.1007/bf00265193"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1467-7652.2004.00067.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022960382"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.85.14.5131", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025804462"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/b:molb.0000038005.73265.61", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026199000", 
              "https://doi.org/10.1023/b:molb.0000038005.73265.61"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.94.6.2117", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032218295"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-4020-2333-0_11", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037734756", 
              "https://doi.org/10.1007/978-1-4020-2333-0_11"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00232724", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039279479", 
              "https://doi.org/10.1007/bf00232724"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00232724", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039279479", 
              "https://doi.org/10.1007/bf00232724"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/aob/mch117", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043604990"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1008855922283", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049553652", 
              "https://doi.org/10.1023/a:1008855922283"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1076878580", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077498858", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2007-01", 
        "datePublishedReg": "2007-01-01", 
        "description": "The presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. In citrus, selection using the selectable marker gene nptII, that confers resistance to the antibiotic kanamycin, is in general very effective. An attractive alternative is offered by the MAT system (Multi-Auto-Transformation), which combines the ipt gene for positive selection with the recombinase system R/RS for removal of marker genes from transgenic cells after transformation. Transformation with a MAT vector has been attempted in two citrus genotypes, Pineapple sweet orange (Citrus sinensis L. Osb.) and Carrizo citrange (C. sinensis L. Osb. x Poncirus trifoliata L. Raf.). Results indicated that the IPT phenotype was clearly distinguishable in sweet orange but not in citrange, and that excision was not always efficient and precise. Nevertheless, the easy visual detection of the IPT phenotype combined with the higher transformation efficiency achieved in sweet orange using this system open interesting perspectives for the generation of marker-free transgenic citrus plants.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s00299-006-0197-3", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1124809", 
            "issn": [
              "0721-7714", 
              "1432-203X"
            ], 
            "name": "Plant Cell Reports", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "26"
          }
        ], 
        "name": "Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination", 
        "pagination": "39-45", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "3442376fdc23c7874464141cc5c57b490b02b5d6732b15fff54329a781b705fc"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "16927091"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9880970"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00299-006-0197-3"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1010968345"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00299-006-0197-3", 
          "https://app.dimensions.ai/details/publication/pub.1010968345"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T14:30", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000373_0000000373/records_13093_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://link.springer.com/10.1007%2Fs00299-006-0197-3"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00299-006-0197-3'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00299-006-0197-3'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00299-006-0197-3'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00299-006-0197-3'


     

    This table displays all metadata directly associated to this object as RDF triples.

    197 TRIPLES      21 PREDICATES      60 URIs      33 LITERALS      21 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00299-006-0197-3 schema:about N03dddb710c7c4b5aa4ceae1e22dfdec4
    2 N0854eec28df74e879e494e56e8f82963
    3 N124d20db73a84d1d8e71ec2b983d99b1
    4 N2ba2f23927c6485a8a33b122293773eb
    5 N2e838040b29a44df93a56fb26e32974f
    6 N3ee60571dbad4f328a150c3b119446c8
    7 N82868aca193148179fa93a7989aa85c6
    8 N83d55f77a1ab447a9cddb012b6aed162
    9 Na3c0026aeb3445fd8d79786c34106208
    10 Na8274b19f4454a11b66dbe8a85eff451
    11 Nfbea867f7a9943fc8c94c9374c094381
    12 Nff4a1d291064420bac8fe45485e07cce
    13 anzsrc-for:06
    14 anzsrc-for:0604
    15 schema:author Nf3db3c07f7ea4f17a9c06038fdbde5db
    16 schema:citation sg:pub.10.1007/978-1-4020-2333-0_11
    17 sg:pub.10.1007/bf00232724
    18 sg:pub.10.1007/bf00265193
    19 sg:pub.10.1007/bf02712670
    20 sg:pub.10.1007/s001220051244
    21 sg:pub.10.1007/s002990050570
    22 sg:pub.10.1007/s002990050688
    23 sg:pub.10.1023/a:1008855922283
    24 sg:pub.10.1023/a:1020308216747
    25 sg:pub.10.1023/b:molb.0000038005.73265.61
    26 sg:pub.10.1038/ng1722
    27 https://app.dimensions.ai/details/publication/pub.1076878580
    28 https://app.dimensions.ai/details/publication/pub.1077498858
    29 https://doi.org/10.1046/j.1365-313x.2002.01272.x
    30 https://doi.org/10.1073/pnas.85.14.5131
    31 https://doi.org/10.1073/pnas.94.6.2117
    32 https://doi.org/10.1093/aob/mch117
    33 https://doi.org/10.1093/treephys/20.17.1183
    34 https://doi.org/10.1111/j.1467-7652.2004.00067.x
    35 schema:datePublished 2007-01
    36 schema:datePublishedReg 2007-01-01
    37 schema:description The presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. In citrus, selection using the selectable marker gene nptII, that confers resistance to the antibiotic kanamycin, is in general very effective. An attractive alternative is offered by the MAT system (Multi-Auto-Transformation), which combines the ipt gene for positive selection with the recombinase system R/RS for removal of marker genes from transgenic cells after transformation. Transformation with a MAT vector has been attempted in two citrus genotypes, Pineapple sweet orange (Citrus sinensis L. Osb.) and Carrizo citrange (C. sinensis L. Osb. x Poncirus trifoliata L. Raf.). Results indicated that the IPT phenotype was clearly distinguishable in sweet orange but not in citrange, and that excision was not always efficient and precise. Nevertheless, the easy visual detection of the IPT phenotype combined with the higher transformation efficiency achieved in sweet orange using this system open interesting perspectives for the generation of marker-free transgenic citrus plants.
    38 schema:genre research_article
    39 schema:inLanguage en
    40 schema:isAccessibleForFree false
    41 schema:isPartOf N55baf2874a1242fb85db3c2a4231196f
    42 Nd29f4b65d9444d65a905d5e7fe14544b
    43 sg:journal.1124809
    44 schema:name Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination
    45 schema:pagination 39-45
    46 schema:productId N147a83d5d1574ffd879e971b2cb0ad4a
    47 Nc203866570a64c2680e4caa1fb054c0e
    48 Nc41236dfb14f4b7c9006c83eb9e841ee
    49 Nd65e79ad760f4a7487a6b5ad6cdef7aa
    50 Ne96725cfd98d49d0a1b419b2e29685ed
    51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010968345
    52 https://doi.org/10.1007/s00299-006-0197-3
    53 schema:sdDatePublished 2019-04-11T14:30
    54 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    55 schema:sdPublisher Nb09a3dd9888a4103bb29e031e293b979
    56 schema:url https://link.springer.com/10.1007%2Fs00299-006-0197-3
    57 sgo:license sg:explorer/license/
    58 sgo:sdDataset articles
    59 rdf:type schema:ScholarlyArticle
    60 N03dddb710c7c4b5aa4ceae1e22dfdec4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    61 schema:name Recombinases
    62 rdf:type schema:DefinedTerm
    63 N0854eec28df74e879e494e56e8f82963 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    64 schema:name Alkyl and Aryl Transferases
    65 rdf:type schema:DefinedTerm
    66 N124d20db73a84d1d8e71ec2b983d99b1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    67 schema:name Plant Shoots
    68 rdf:type schema:DefinedTerm
    69 N147a83d5d1574ffd879e971b2cb0ad4a schema:name pubmed_id
    70 schema:value 16927091
    71 rdf:type schema:PropertyValue
    72 N2ba2f23927c6485a8a33b122293773eb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    73 schema:name Citrus sinensis
    74 rdf:type schema:DefinedTerm
    75 N2e838040b29a44df93a56fb26e32974f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    76 schema:name Genetic Vectors
    77 rdf:type schema:DefinedTerm
    78 N3ee60571dbad4f328a150c3b119446c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    79 schema:name Genetic Markers
    80 rdf:type schema:DefinedTerm
    81 N55baf2874a1242fb85db3c2a4231196f schema:issueNumber 1
    82 rdf:type schema:PublicationIssue
    83 N82868aca193148179fa93a7989aa85c6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    84 schema:name Base Sequence
    85 rdf:type schema:DefinedTerm
    86 N83d55f77a1ab447a9cddb012b6aed162 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    87 schema:name Transformation, Genetic
    88 rdf:type schema:DefinedTerm
    89 N950eef5cc9ec4dbea2133a54a113a0f5 rdf:first sg:person.01032457452.34
    90 rdf:rest Nee07b4274583433cbc2d8eafdc216023
    91 Na3c0026aeb3445fd8d79786c34106208 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    92 schema:name Recombination, Genetic
    93 rdf:type schema:DefinedTerm
    94 Na8274b19f4454a11b66dbe8a85eff451 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    95 schema:name Plants, Genetically Modified
    96 rdf:type schema:DefinedTerm
    97 Nb09a3dd9888a4103bb29e031e293b979 schema:name Springer Nature - SN SciGraph project
    98 rdf:type schema:Organization
    99 Nc203866570a64c2680e4caa1fb054c0e schema:name readcube_id
    100 schema:value 3442376fdc23c7874464141cc5c57b490b02b5d6732b15fff54329a781b705fc
    101 rdf:type schema:PropertyValue
    102 Nc41236dfb14f4b7c9006c83eb9e841ee schema:name dimensions_id
    103 schema:value pub.1010968345
    104 rdf:type schema:PropertyValue
    105 Nd29f4b65d9444d65a905d5e7fe14544b schema:volumeNumber 26
    106 rdf:type schema:PublicationVolume
    107 Nd65e79ad760f4a7487a6b5ad6cdef7aa schema:name nlm_unique_id
    108 schema:value 9880970
    109 rdf:type schema:PropertyValue
    110 Ne96725cfd98d49d0a1b419b2e29685ed schema:name doi
    111 schema:value 10.1007/s00299-006-0197-3
    112 rdf:type schema:PropertyValue
    113 Nee07b4274583433cbc2d8eafdc216023 rdf:first sg:person.0743161043.55
    114 rdf:rest rdf:nil
    115 Nf3db3c07f7ea4f17a9c06038fdbde5db rdf:first sg:person.01263224217.20
    116 rdf:rest N950eef5cc9ec4dbea2133a54a113a0f5
    117 Nfbea867f7a9943fc8c94c9374c094381 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name Citrus
    119 rdf:type schema:DefinedTerm
    120 Nff4a1d291064420bac8fe45485e07cce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name Molecular Sequence Data
    122 rdf:type schema:DefinedTerm
    123 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    124 schema:name Biological Sciences
    125 rdf:type schema:DefinedTerm
    126 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    127 schema:name Genetics
    128 rdf:type schema:DefinedTerm
    129 sg:journal.1124809 schema:issn 0721-7714
    130 1432-203X
    131 schema:name Plant Cell Reports
    132 rdf:type schema:Periodical
    133 sg:person.01032457452.34 schema:affiliation https://www.grid.ac/institutes/grid.419276.f
    134 schema:familyName Cervera
    135 schema:givenName Magdalena
    136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01032457452.34
    137 rdf:type schema:Person
    138 sg:person.01263224217.20 schema:affiliation https://www.grid.ac/institutes/grid.419276.f
    139 schema:familyName Ballester
    140 schema:givenName Alida
    141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01263224217.20
    142 rdf:type schema:Person
    143 sg:person.0743161043.55 schema:affiliation https://www.grid.ac/institutes/grid.419276.f
    144 schema:familyName Peña
    145 schema:givenName Leandro
    146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0743161043.55
    147 rdf:type schema:Person
    148 sg:pub.10.1007/978-1-4020-2333-0_11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037734756
    149 https://doi.org/10.1007/978-1-4020-2333-0_11
    150 rdf:type schema:CreativeWork
    151 sg:pub.10.1007/bf00232724 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039279479
    152 https://doi.org/10.1007/bf00232724
    153 rdf:type schema:CreativeWork
    154 sg:pub.10.1007/bf00265193 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021579003
    155 https://doi.org/10.1007/bf00265193
    156 rdf:type schema:CreativeWork
    157 sg:pub.10.1007/bf02712670 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003131267
    158 https://doi.org/10.1007/bf02712670
    159 rdf:type schema:CreativeWork
    160 sg:pub.10.1007/s001220051244 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015745462
    161 https://doi.org/10.1007/s001220051244
    162 rdf:type schema:CreativeWork
    163 sg:pub.10.1007/s002990050570 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007201059
    164 https://doi.org/10.1007/s002990050570
    165 rdf:type schema:CreativeWork
    166 sg:pub.10.1007/s002990050688 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012967220
    167 https://doi.org/10.1007/s002990050688
    168 rdf:type schema:CreativeWork
    169 sg:pub.10.1023/a:1008855922283 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049553652
    170 https://doi.org/10.1023/a:1008855922283
    171 rdf:type schema:CreativeWork
    172 sg:pub.10.1023/a:1020308216747 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004890324
    173 https://doi.org/10.1023/a:1020308216747
    174 rdf:type schema:CreativeWork
    175 sg:pub.10.1023/b:molb.0000038005.73265.61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026199000
    176 https://doi.org/10.1023/b:molb.0000038005.73265.61
    177 rdf:type schema:CreativeWork
    178 sg:pub.10.1038/ng1722 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011139424
    179 https://doi.org/10.1038/ng1722
    180 rdf:type schema:CreativeWork
    181 https://app.dimensions.ai/details/publication/pub.1076878580 schema:CreativeWork
    182 https://app.dimensions.ai/details/publication/pub.1077498858 schema:CreativeWork
    183 https://doi.org/10.1046/j.1365-313x.2002.01272.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1019828039
    184 rdf:type schema:CreativeWork
    185 https://doi.org/10.1073/pnas.85.14.5131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025804462
    186 rdf:type schema:CreativeWork
    187 https://doi.org/10.1073/pnas.94.6.2117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032218295
    188 rdf:type schema:CreativeWork
    189 https://doi.org/10.1093/aob/mch117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043604990
    190 rdf:type schema:CreativeWork
    191 https://doi.org/10.1093/treephys/20.17.1183 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002652786
    192 rdf:type schema:CreativeWork
    193 https://doi.org/10.1111/j.1467-7652.2004.00067.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022960382
    194 rdf:type schema:CreativeWork
    195 https://www.grid.ac/institutes/grid.419276.f schema:alternateName Instituto Valenciano de Investigaciones Agrarias
    196 schema:name Department Plant Protection and Biotechnology, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial 46113-Moncada, Valencia, Spain
    197 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...