Isolation of cytoplasts from Satsuma mandarin (Citrus unshiu Marc.) and production of alloplasmic hybrid calluses via cytoplast–protoplast fusion View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-06

AUTHORS

X. Y. Xu, J. H. Liu, X. X. Deng

ABSTRACT

Cytoplasm of Satsuma mandarin (Citrus unshiu Marc.) is known to influence seedlessness. Transfer of cytoplasm to a seedy cultivar could possibly lead to the production of seedless citrus fruits. In the present paper cytoplasts were isolated from cell suspension-derived protoplasts of Satsuma mandarin via ultra-centrifugation in a discontinuous gradient. No nucleus could be detected in the cytoplasts by DAPI (4', 6-diamidino-2-phenylindole) staining compared with normal protoplasts. The cytoplasts, with high viability and small size, did not divide during solid embedding culture. Cytoplasts of Satsuma mandarin were electrically fused with embryogenic protoplasts of Murcott tangor (C. reticulata x C. sinensis), which led to regeneration of several cell lines. Flow cytometry (FCM) indicated that the cell lines were diploids. Simple sequence repeats (SSR) and cleaved amplified polymorphism sequence (CAPS) showed that the cell lines got their nuclear DNA from the protoplast parent, whereas the cytoplast parent donated the mtDNA, confirming transfer of mtDNA from Satsuma mandarin into Murcott tangor via cytoplast-protoplast fusion though no polymorphism was detected in chloroplast DNA between the fusion partners. This is the first report on isolation and characterization of cytoplasts, together with cytoplast-protoplast fusion in Citrus, which has a potential for citrus cultivar improvement involving cytoplasm transfer via cytoplast-protoplast fusion. More... »

PAGES

533-539

References to SciGraph publications

  • 2005-01. Allotetraploid hybrids produced by protoplast fusion for seedless triploid Citrus breeding in EUPHYTICA
  • 2000-11. Somatic hybridization in citrus: An effective tool to facilitate variety improvement in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 1994-02. Organelle inheritance in plants in HEREDITY
  • 1978-11. Investigations on the transfer of isolated nuclei into plant protoplasts in THEORETICAL AND APPLIED GENETICS
  • 2002-11. Molecular characterization of the nuclear and cytoplasmic genomes of intergeneric diploid plants from cell fusion between Microcitrus papuana and Rough lemon in PLANT CELL REPORTS
  • 2005-07. Intergeneric somatic hybridization and its application to crop genetic improvement in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1997-04. Integration of trinucleotide microsatellites into a linkage map of Citrus in THEORETICAL AND APPLIED GENETICS
  • 1990-09. Intergeneric transfer of cytoplasmic male sterility between Raphanus sativus (cms line) and Brassica napus through cytoplast-protoplast fusion in THEORETICAL AND APPLIED GENETICS
  • 1995-02. Intergeneric symmetric and asymmetric somatic hybridization in Festuca and Lolium in EUPHYTICA
  • 2004-05. Amplified fragment length polymorphism for variety identification and genetic diversity assessment in oleander (Nerium oleander L.) in EUPHYTICA
  • 1989-11. Protoplast-fusion-mediated transfer of organelles from Microcitrus into Citrus and regeneration of novel alloplasmic trees in THEORETICAL AND APPLIED GENETICS
  • 1997-02. Direct transfer of a cold-tolerant Ogura male-sterile cytoplasm into cabbage (Brassica oleracea ssp. capitata) via protoplast fusion in THEORETICAL AND APPLIED GENETICS
  • 1987-12. Citrus cybrids: production by donor-recipient protoplast-fusion and verification by mitochondrial-DNA restriction profiles in THEORETICAL AND APPLIED GENETICS
  • 1999-11. Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2002-06. Cell size as a morphological marker to calculate the mitotic index and ploidy level of citrus callus in PLANT CELL REPORTS
  • 2002-05. Regeneration and analysis of citrus interspecific mixoploid hybrid plants from asymmetric somatic hybridization in EUPHYTICA
  • 1982-04. Cytoplast-protoplast fusion for interspecific chloroplast transfer in Nicotiana in MOLECULAR GENETICS AND GENOMICS
  • 1993-04. Glycerol stimulation of chlorophyll synthesis, embryogenesis, and carboxylation and sucrose metabolism enzymes in nucellar callus of ‘Hamlin’ sweet orange in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1991-04. Organelle transfer by microfusion of defined protoplast-cytoplast pairs in THEORETICAL AND APPLIED GENETICS
  • 1986-04. A technique for bulk production of cytoplasts and miniprotoplasts from suspension culture-derived protoplasts in PLANT CELL REPORTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00299-005-0057-6

    DOI

    http://dx.doi.org/10.1007/s00299-005-0057-6

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1045285216

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16477406


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