Increased resistance to crown rust disease in transgenic Italian ryegrass (Lolium multiflorum Lam.) expressing the rice chitinase gene View Full Text


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Article Info

DATE

2004-12-15

AUTHORS

Wataru Takahashi, Masahiro Fujimori, Yuichi Miura, Toshinori Komatsu, Yoko Nishizawa, Tadaaki Hibi, Tadashi Takamizo

ABSTRACT

We introduced the rice chitinase (Cht-2; RCC2) gene into calli of Italian ryegrass (Lolium multiflorum Lam.), with a hygromycin phosphotransferase (HPT) gene as a selectable marker, by particle bombardment. Hygromycin-resistant calli were selected and transferred to regeneration medium for shoot formation. Polymerase chain reaction (PCR) analysis revealed regenerants containing the HPT gene. The RCC2 gene was detected in 65.5% of those regenerants. Southern hybridization detected both HPT and RCC2 genes and indicated that the transgenic plants were independently transformed. Expression of the RCC2 gene in the transgenic plants was confirmed by Northern hybridization, reverse transcription–PCR and Western blotting. Bioassay of detached leaves indicated increased resistance to crown rust (Puccinia coronata) in transgenic plants, which exhibited higher chitinase activity than a nontransgenic plant. More... »

PAGES

811-818

References to SciGraph publications

  • 1997-03. Transgenic Italian ryegrass (Lolium multiflorum) plants from microprojectile bombardment of embryogenic suspension cells in PLANT CELL REPORTS
  • 1998-01. Transgenic cucumber plants harboring a rice chitinase gene exhibit enhanced resistance to gray mold (Botrytis cinerea) in PLANT CELL REPORTS
  • 2004-02-13. Modification of senescence in ryegrass transformed with IPT under the control of a monocot senescence-enhanced promoter in PLANT CELL REPORTS
  • 1999-08. Enhanced resistance to blast (Magnaporthe grisea) in transgenic Japonica rice by constitutive expression of rice chitinase in THEORETICAL AND APPLIED GENETICS
  • 1993-10. Sequence variation, differential expression and chromosomal location of rice chitinase genes in MOLECULAR GENETICS AND GENOMICS
  • 2001-02-03. Altered fructan accumulation in transgenic Lolium multiflorum plants expressing a Bacillus subtilis sacB gene in PLANT CELL REPORTS
  • 1999-09. Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene in THEORETICAL AND APPLIED GENETICS
  • 2002-12-04. Expression of pokeweed antiviral proteins in creeping bentgrass in PLANT CELL REPORTS
  • 2003-08. The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene, confers antifungal activity against Botrytis cinerea to Transgenic tobacco in TRANSGENIC RESEARCH
  • 2000-06. Transgenic grapevine plants expressing a rice chitinase with enhanced resistance to fungal pathogens in PLANT CELL REPORTS
  • 2000-03. Expression of active barley seed ribosome-inactivating protein in transgenic wheat in THEORETICAL AND APPLIED GENETICS
  • 1996-09. Constitutive expression of an inducible β-1,3-glucanase in alfalfa reduces disease severity caused by the oomycete pathogenPhytophthora megasperma f. spmedicaginis, but does not reduce disease severity of chitin-containing fungi in TRANSGENIC RESEARCH
  • 1999-05. Genetic engineering of wheat for increased resistance to powdery mildew disease in THEORETICAL AND APPLIED GENETICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00299-004-0900-1

    DOI

    http://dx.doi.org/10.1007/s00299-004-0900-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1033760437

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15599752


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