Green fluorescent protein as a vital elimination marker to easily screen marker-free transgenic progeny derived from plants co-transformed with a ... View Full Text


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Article Info

DATE

2005-02

AUTHORS

Songbiao Chen, Xugang Li, Xiang Liu, Hongling Xu, Kun Meng, Guifang Xiao, Xiaoli Wei, Feng Wang, Zhen Zhu

ABSTRACT

We investigated the potential of a novel double T-DNA vector for generating marker-free transgenic plants. Co-transformation methods using a double T-DNA vector or using mixture of two Agrobacterium tumefaciens strains were compared, and showed that the double T-DNA vector method could produce marker-free transgenic tobacco (Nicotiana tabacum L.) plants more efficiently. A dual marker double T-DNA vector was then constructed by assembling the green fluorescent protein (GFP) gene mgfp5 and the neomycin phosphotransferase gene nptII into the same T-DNA. The frequency of co-transformants produced by this vector was 56.3%. Co-expression of mgfp5 and nptII was found in 28 out of 29 T1 lines, and segregation of the reporter beta-glucuronidase gene, gusA, from mgfp5 to nptII was found in 12 out of 29 T1 lines. Therefore, GFP could be used as a vital marker to improve the transformation efficiency and to easily monitor the segregation of marker genes, thus facilitating screening of marker-free progeny. More... »

PAGES

625-631

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00299-004-0853-4

DOI

http://dx.doi.org/10.1007/s00299-004-0853-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037109083

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15449016


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