Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-11

AUTHORS

J-S. Han, D-G. Oh, I-G. Mok, H-G. Park, C. K. Kim

ABSTRACT

Using cotyledon explants excised from seedlings germinated in vitro, an efficient plant regeneration system via organogenesis was established for bottle gourd (Lagenaria siceraria Standl.). Maximum shoot regeneration was obtained when the proximal parts of cotyledons from 4-day-old seedlings were cultured on MS medium with 3 mg/l BA and 0.5 mg/l AgNO(3) under a 16-h photoperiod. After 3-4 weeks of culture, 21.9-80.7% of explants from the five cultivars regenerated shoots. Adventitious shoots were successfully rooted on a half-strength MS medium with 0.1 mg/l IAA for 2-3 weeks. Flow cytometric analysis revealed that most of the regenerated plants derived from culture on medium with AgNO(3) were diploid. More... »

PAGES

291-296

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00299-004-0846-3

DOI

http://dx.doi.org/10.1007/s00299-004-0846-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009883136

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15365758


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