Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.) View Full Text


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Article Info

DATE

2004-11

AUTHORS

J-S. Han, D-G. Oh, I-G. Mok, H-G. Park, C. K. Kim

ABSTRACT

Using cotyledon explants excised from seedlings germinated in vitro, an efficient plant regeneration system via organogenesis was established for bottle gourd (Lagenaria siceraria Standl.). Maximum shoot regeneration was obtained when the proximal parts of cotyledons from 4-day-old seedlings were cultured on MS medium with 3 mg/l BA and 0.5 mg/l AgNO(3) under a 16-h photoperiod. After 3-4 weeks of culture, 21.9-80.7% of explants from the five cultivars regenerated shoots. Adventitious shoots were successfully rooted on a half-strength MS medium with 0.1 mg/l IAA for 2-3 weeks. Flow cytometric analysis revealed that most of the regenerated plants derived from culture on medium with AgNO(3) were diploid. More... »

PAGES

291-296

References to SciGraph publications

  • 1997-10. Influence of silver nitrate (ethylene inhibitor) on cucumber in vitro shoot regeneration in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
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  • 1994-06. Enhancement of shoot regeneration from cotyledon explants of Brassica rapa ssp oleifera through pretreatment with auxin and cytokinin and use of ethylene inhibitors in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1988-03. Callus growth and plant regeneration in diverse cultivars of cucumber (Cucumis sativus L.) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1995-12. Characterization and classification of different genotypes in a population of Cucumis melo based on their ability to regenerate shoots from leaf explants in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1998-01. Regeneration and frequency of tetraploid variants of Cucumis metuliferus are affected by explant induction on semi-solid medium versus the liquid/ membrane system in PLANT CELL REPORTS
  • 1991-02. High efficiency plant regeneration from cotyledons of watermelon (Citrullus vulgaris Schrad) in PLANT CELL REPORTS
  • 2003-08. Ethylene inhibitors and low kanamycin concentrations improve adventitious regeneration from apricot leaves in PLANT CELL REPORTS
  • 2003-11. In vitro micropropagation of a Cucurbita interspecific hybrid cultivar – a root stock plant in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1999-09. Dark pretreatment improves adventitious shoot organogenesis from cotyledons of diploid watermelon in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00299-004-0846-3

    DOI

    http://dx.doi.org/10.1007/s00299-004-0846-3

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1009883136

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15365758


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