A simple and rapid Agrobacterium-mediated transformation protocol for cotton (Gossypium hirsutum L.): Embryogenic calli as a source to generate large ... View Full Text


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Article Info

DATE

2004-02

AUTHORS

S. Leelavathi, V. G. Sunnichan, R. Kumria, G. P. Vijaykanth, R. K. Bhatnagar, V. S. Reddy

ABSTRACT

A protocol is presented for efficient transformation and regeneration of cotton. Embryogenic calli co-cultivated with Agrobacterium carrying cry1Ia5 gene were cultured under dehydration stress and antibiotic selection for 3-6 weeks to generate several transgenic embryos. An average of 75 globular embryo clusters were observed on selection plates and these embryos were cultured on multiplication medium followed by development of cotyledonary embryos on embryo maturation medium to obtain an average of 12 plants per Petri plate of co-cultivated callus. About 83% of these plants have been confirmed to be transgenic by Southern blot analysis. An efficiency of ten kanamycin-resistant plants per Petri plate of co-cultivated embryogenic callus was obtained. The simplicity of the procedure and the efficiency of the initial material allow transformation of any variety where a single regenerating embryogenic callus line can be obtained. In addition, multiple transformations can be performed either simultaneously or sequentially. The method is extremely simple, reliable, efficient, and much less laborious than any other existing method for cotton transformation. More... »

PAGES

465-470

References to SciGraph publications

  • 2003-06. Slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L. cv. Coker 310 FR) in PLANT CELL REPORTS
  • 2001-08. Transgenic cotton: factors influencing Agrobacterium-mediated transformation and regeneration in MOLECULAR BREEDING
  • 1999-02. Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex in THEORETICAL AND APPLIED GENETICS
  • 1987-03. Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants in PLANT MOLECULAR BIOLOGY
  • 1996-08. Regeneration of plants from cryopreserved embryogenic cell suspension and callus cultures of cotton (Gossypium hirsutum L.) in PLANT CELL REPORTS
  • 1991-08. Improved culture conditions for somatic embryogenesis from Asparagus officinalis L. using an aseptic ventilative filter in PLANT CELL REPORTS
  • 1997-11. Transgenic cotton resistant to herbicide bialaphos in TRANSGENIC RESEARCH
  • 2003-03. High-frequency somatic embryo production and maturation into normal plants in cotton (Gossypium hirsutum) through metabolic stress in PLANT CELL REPORTS
  • 1998-12. Transformation of Nicotiana tabacum with a native cry1Ia5 gene confers complete protection against Heliothis armigera in MOLECULAR BREEDING
  • 1991-05. Regeneration of Gossypium hirsutum and G. barbadense from shoot apex tissues for transformation in PLANT CELL REPORTS
  • 2003-04. Development of a highly regenerable elite Acala cotton (Gossypium hirsutum cv. Maxxa) – a step towards genotype-independent regeneration in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1988-10. Plant regeneration from somatic embryogenic suspension cultures of cotton (Gossypium hirsutum L.) in PLANT CELL REPORTS
  • 1996-02. Stimulatory effect of water stress on plant regeneration in aromatic Indica rice varieties in PLANT CELL REPORTS
  • 1987-03. Genetically Transformed Cotton (Gossypium Hirsutum L.) Plants in NATURE BIOTECHNOLOGY
  • 1997-09. Transformation of rice mediated by Agrobacterium tumefaciens in PLANT MOLECULAR BIOLOGY
  • 1996-12. Herbicide-resistant Acala and Coker cottons transformed with a native gene encoding mutant forms of acetohydroxyacid synthase in MOLECULAR BREEDING
  • 2000-05. High-frequency stable transformation of cotton (Gossypium hirsutum L.) by particle bombardment of embryogenic cell suspension cultures in PLANT CELL REPORTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00299-003-0710-x

    DOI

    http://dx.doi.org/10.1007/s00299-003-0710-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1032861855

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/13680138


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