Slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L. cv. Coker 310 FR) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-06

AUTHORS

B. Chaudhary, S. Kumar, K. V. S. K. Prasad, G. S. Oinam, P. K. Burma, D. Pental

ABSTRACT

In Agrobacterium-mediated genetic transformation of cotton (Gossypium hirsutum L. cv. Coker 310FR) the frequency at which somatic embryos were converted to plantlets was significantly improved by subjecting the embryos to slow physical desiccation. We used Agrobacterium strain GV3101 containing the binary vector pGSFR with the nos-nptII gene for in vitro selection and the 35S gus-int fragment as a reporter to optimize the transformation protocol. Although the concentration of kanamycin was reduced during embryogenesis and embryo maturation, even at the lower levels somatic embryos were predominantly abnormal, showing hypertrophy and reduced or fused cotyledons or poor radicle ends. A majority of these embryos (more than 75%) were beta-glucuronidase (GUS)-positive. Embryos with an abnormal appearance showed a very poor conversion to plantlets. However, these embryos, when subjected to slow physical desiccation followed by transfer to fresh medium, regenerated single or multiple shoots from the cotyledonary end. These shoots could be grafted on wild-type seedling stocks in vitro, which, following their transfer to soil, developed normally and set seeds. Regenerated plants tested positive for the transgene by Southern analysis. An overall scheme for the high-frequency production of cotton transgenics from both normal and abnormal appearing somatic embryos is presented. More... »

PAGES

955-960

References to SciGraph publications

  • 2001-08. Transgenic cotton: factors influencing Agrobacterium-mediated transformation and regeneration in MOLECULAR BREEDING
  • 1990-01. Construction of an intron-containing marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation in MOLECULAR GENETICS AND GENOMICS
  • 2001-02. Effects of kanamycin on tissue culture and somatic embryogenesis in cotton in PLANT GROWTH REGULATION
  • 1998-02. Rapid in-vitro plant regeneration of cotton (Gossypium hirsutum L.) in PLANT CELL REPORTS
  • 1990-10. Insect Resistant Cotton Plants in BIO/TECHNOLOGY
  • 1990-03. Transformation of cotton (Gossypium hirsutum L.) via particle bombardment in PLANT CELL REPORTS
  • 1996-12. Herbicide-resistant Acala and Coker cottons transformed with a native gene encoding mutant forms of acetohydroxyacid synthase in MOLECULAR BREEDING
  • 1987-03. Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants in PLANT MOLECULAR BIOLOGY
  • 1998-11. A genetic approach to in vitro regeneration of non-regenerating cotton (Gossypium hirsutum L.) cultivars in PLANT CELL REPORTS
  • 1999-06. In vitro shoot-tip grafting improves recovery of cotton plants from culture in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1987-03. Genetically Transformed Cotton (Gossypium Hirsutum L.) Plants in NATURE BIOTECHNOLOGY
  • 1986-09. The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector in MOLECULAR GENETICS AND GENOMICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00299-003-0613-x

    DOI

    http://dx.doi.org/10.1007/s00299-003-0613-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1032819279

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12835904


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