High-frequency somatic embryo production and maturation into normal plants in cotton (Gossypium hirsutum) through metabolic stress View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-03

AUTHORS

R. Kumria, V. G. Sunnichan, D. K. Das, S. K. Gupta, V. S. Reddy, R. K. Bhatnagar, S. Leelavathi

ABSTRACT

A highly efficient somatic embryo production and maturation procedure has been developed to regenerate plantlets from cotton ( Gossypium hirsutum). This procedure involves the acceleration of differentiation through manipulations of nutrient and microenvironment conditions. Embryogenic calli, initiated from hypocotyls or cotyledonary leaf sections on MS medium containing 0.1 mg/l 2,4 dichlorophenoxyacetic acid, 0.5 mg/l kinetin, and 3% maltose produced globular-stage somatic embryos when transferred to hormone-free MS medium supplemented with high concentrations of nitrate. Subculture of globular embryos on hormone-free MS medium led to the development of torpedo- and cotyledonary-stage at a low frequency (two to four per plate) with the majority of embryos lacking further growth or entering into the dedifferentiation stage. Significant improvement in embryogenesis (two- to threefold) was achieved when calli were cultured on 1/5-strength MS medium irrespective of stress treatment. However, the frequency of globular embryos developing into normal plantlets improved considerably (20-24 per plate) when cultured on filter paper placed on MS medium. In this procedure, about 33% of globular embryos not only developed into the cotyledonary stage but rooted simultaneously, eliminating a separate rooting step. More than 70% of cotyledonary embryos developed into normal plantlets when cultured on full- strength MS medium containing 0.05 mg/l gibberellic acid. More... »

PAGES

635-639

References to SciGraph publications

  • 1996-08. Regeneration of plants from cryopreserved embryogenic cell suspension and callus cultures of cotton (Gossypium hirsutum L.) in PLANT CELL REPORTS
  • 1990-09. Genetic control of somatic embryogenesis in cotton petiole callus cultures in EUPHYTICA
  • 1987-03. Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants in PLANT MOLECULAR BIOLOGY
  • 1987-06. Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.) in PLANT CELL REPORTS
  • 1991-08. Improved culture conditions for somatic embryogenesis from Asparagus officinalis L. using an aseptic ventilative filter in PLANT CELL REPORTS
  • 1988-03. Somatic embryogenesis in cotton (Gossypium). II. Requirements for embryo development and plant regeneration in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1991-05. Regeneration of Gossypium hirsutum and G. barbadense from shoot apex tissues for transformation in PLANT CELL REPORTS
  • 1979-01. Somatic embryogenesis in suspension cultures of Gossypium klotzschianum anderss in PLANTA
  • 1988-10. Plant regeneration from somatic embryogenic suspension cultures of cotton (Gossypium hirsutum L.) in PLANT CELL REPORTS
  • 1986-12. Somatic embryogenesis from leaf and petiole callus cultures of Gossypium hirsutum L. in PLANT CELL REPORTS
  • 1986-06. Characterization of somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.) in PLANT CELL REPORTS
  • 1989-03. Genotype specificity of the somatic embryogenesis response in cotton in PLANT CELL REPORTS
  • 1995. Morphogenic Aspects of Somatic Embryogenesis in IN VITRO EMBRYOGENESIS IN PLANTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00299-002-0554-9

    DOI

    http://dx.doi.org/10.1007/s00299-002-0554-9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1075296688

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12789412


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    36 schema:description A highly efficient somatic embryo production and maturation procedure has been developed to regenerate plantlets from cotton ( Gossypium hirsutum). This procedure involves the acceleration of differentiation through manipulations of nutrient and microenvironment conditions. Embryogenic calli, initiated from hypocotyls or cotyledonary leaf sections on MS medium containing 0.1 mg/l 2,4 dichlorophenoxyacetic acid, 0.5 mg/l kinetin, and 3% maltose produced globular-stage somatic embryos when transferred to hormone-free MS medium supplemented with high concentrations of nitrate. Subculture of globular embryos on hormone-free MS medium led to the development of torpedo- and cotyledonary-stage at a low frequency (two to four per plate) with the majority of embryos lacking further growth or entering into the dedifferentiation stage. Significant improvement in embryogenesis (two- to threefold) was achieved when calli were cultured on 1/5-strength MS medium irrespective of stress treatment. However, the frequency of globular embryos developing into normal plantlets improved considerably (20-24 per plate) when cultured on filter paper placed on MS medium. In this procedure, about 33% of globular embryos not only developed into the cotyledonary stage but rooted simultaneously, eliminating a separate rooting step. More than 70% of cotyledonary embryos developed into normal plantlets when cultured on full- strength MS medium containing 0.05 mg/l gibberellic acid.
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