Highly efficient expression of rabbit neutrophil peptide-1 gene in Chlorella ellipsoidea cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-07

AUTHORS

Ying Chen, Yiqin Wang, Yongru Sun, Liming Zhang, Wenbin Li

ABSTRACT

A highly efficient system was developed for the expression of foreign genes in Chlorella ellipsoidea cells. The effect of five promoters on the expression efficiency of beta-glucuronidase (GUS) gene was evaluated by transient expression of the UidA gene. Among these promoters, Ubiquitin-omega was found to be the most efficient and was selected to drive the expression of foreign genes in Chlorella cells. A gene encoding the mature rabbit neutrophil peptide-1 (NP-1) was introduced into the cells. Integration of the gene for NP-1 into the Chlorella genome was confirmed by PCR and Southern blot analysis. In, vitro anti-microbial testes demonstrated the expression of biologically active NP-1 by the transgenic Chlorella cells. More... »

PAGES

365-370

Journal

TITLE

Current Genetics

ISSUE

5-6

VOLUME

39

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002940100205

DOI

http://dx.doi.org/10.1007/s002940100205

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020123991

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11525411


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