AoHapB, AoHapC and AoHapE, subunits of the Aspergillus oryzae CCAAT-binding complex, are functionally interchangeable with the corresponding subunits in Aspergillus ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-05

AUTHORS

Akimitsu Tanaka, Ken-ichi Kamei, Shinjiro Tanoue, Peter Papagiannopoulos, Stefan Steidl, Axel A. Brakhage, Meryl A. Davis, Michael J. Hynes, Masashi Kato, Tetsuo Kobayashi, Norihiro Tsukagoshi

ABSTRACT

Two genes, AohapB and AohapE, encoding subunits of the Aspergillus oryzae CCAAT-binding complex were cloned and sequenced. The polypeptides encoded by AohapB and AohapE were expressed in Escherichia coli and used to reconstitute a DNA-binding complex with recombinant AoHapC. The DNA-binding activity was observed only in the presence of all three subunits, indicating that AoHapB, AoHapE and AoHapC are essential for CCAAT-binding. Furthermore, introduction of the AohapB, AohapC and AohapE genes into the A. nidulans hapB delta, hapC delta and hapE delta strains, respectively, revealed that the A. oryzae Hap subunits are functionally interchangeable with the corresponding subunits in A. nidulans. More... »

PAGES

175-182

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002940100190

DOI

http://dx.doi.org/10.1007/s002940100190

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034976591

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11409179


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