Prediction of breakthrough curves in packed-bed column as tool for lysozyme isolation using a green bed View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-01-18

AUTHORS

M. Emilia Brassesco, Nadia Woitovich Valetti, Guillermo Picó

ABSTRACT

The adsorption of lysozyme onto alginate–guar gum bed cross-linked with epichlorohydrin has been studied in packed-bed column. Adsorption performance was evaluated with different bed heights, flow rates and initial protein concentrations. The Thomas and bed depth service time mathematical models fitted well the breakthrough curves experimental data with high correlation coefficients. Not modification of the working yield of the bed was observed in progressive ten cycles of reused. Lysozyme recovery of 75% was achieved from white egg with a purification factor around 15 under the condition of bed height 9 cm, flow rate 0.4 mL/min and total protein inlet 10 mg/mL, while the dynamic bed capacity under this experimental condition was 10.87 mg/g hydrated bed. More... »

PAGES

1-17

References to SciGraph publications

Journal

TITLE

Polymer Bulletin

ISSUE

N/A

VOLUME

N/A

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00289-019-02683-5

DOI

http://dx.doi.org/10.1007/s00289-019-02683-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111509086


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