Synthesis of Polystyrene Nanoparticles with Different Surface Modification by Emulsion Polymerization and Measurement of IgG Adsorption and Stability for the ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-01

AUTHORS

Sanghee Kim, Chul Am Kim, Yo Han Choi, Moon Youn Jung

ABSTRACT

Unmodified and surface modified polystyrene (PS) nanoparticles with hydrophilic end group on surface were synthesized by emulsion polymerization for adsorption of a protein (immunoglobulin G, IgG) by passive adsorption and electrostatic attraction for the application on solid-phase immunoassay. The presence of formed IgG-PS particle complex was confirmed by dynamic light scattering (DLS) measurement which shows the increased size of particle (≈178nm and 220nm) after IgG immobilization. Increased Zeta (ζ) potential from -56.7mV to -3.84mV following the IgG immobilization by electrostatic attraction also provides the presence of the formed IgG-PS particle complex. Enzyme-linked immunosorbent assay (ELISA) results demonstrate that the formed IgG-PS complex was stable and the binding strength between IgG and PS was maintained after 1 month from initial IgG-PS particle complex formation. More... »

PAGES

23-32

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00289-008-1010-y

DOI

http://dx.doi.org/10.1007/s00289-008-1010-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020964047


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