Physiological and Molecular Biological Characterization of Ammonia Oxidation of the Heterotrophic Nitrifier Pseudomonas putida View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-10

AUTHORS

Michael Daum, Wolfgang Zimmer, Hans Papen, Karin Kloos, Kerstin Nawrath, Hermann Bothe

ABSTRACT

The heterotrophic nitrifier Pseudomonas putida aerobically oxidized ammonia to hydroxylamine, nitrite, and nitrate. Product formation was accompanied by a small but significant release of NO, whereas N2O evolution could not be detected under the assay conditions employed. The isolate reduced nitrate to nitrite and partially further to NO under anaerobic conditions. Aerobically grown cells utilized gamma-aminobutyrate as a carbon source and as a N-source by ammonification. The physiological experiments, in particular the inhibition pattern by C2H2, indicated that P. putida expressed an ammonia monooxigenase. DNA-hybridization with an amoA gene probe coding for the smaller subunit of the ammonia monooxigenase of Nitrosomonas europaea allowed us to identify, to clone, and to sequence a region with an open reading frame showing distinct sequence similarities to the amoA gene of autotrophic ammonia oxidizers. More... »

PAGES

281-288

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002849900379

DOI

http://dx.doi.org/10.1007/s002849900379

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034296882

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9732537


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aerobiosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ammonia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydroxylamine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nitrates", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Open Reading Frames", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oxidation-Reduction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oxidoreductases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pseudomonas putida", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Restriction Mapping", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Amino Acid", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Fraunhofer Institut f\u00fcr Atmosph\u00e4rische Umweltforschung, Kreuzeckbahnstr. 19, D-82467 Garmisch-Partenkirchen, Germany, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Daum", 
        "givenName": "Michael", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Fraunhofer Institut f\u00fcr Atmosph\u00e4rische Umweltforschung, Kreuzeckbahnstr. 19, D-82467 Garmisch-Partenkirchen, Germany, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zimmer", 
        "givenName": "Wolfgang", 
        "id": "sg:person.01105672124.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105672124.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Fraunhofer Institut f\u00fcr Atmosph\u00e4rische Umweltforschung, Kreuzeckbahnstr. 19, D-82467 Garmisch-Partenkirchen, Germany, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Papen", 
        "givenName": "Hans", 
        "id": "sg:person.012142240251.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012142240251.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Cologne", 
          "id": "https://www.grid.ac/institutes/grid.6190.e", 
          "name": [
            "Botanisches Institut, Universit\u00e4t zu K\u00f6ln, Gyrhofstr. 15, D-50923 K\u00f6ln, Germany, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kloos", 
        "givenName": "Karin", 
        "id": "sg:person.01265432666.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265432666.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Cologne", 
          "id": "https://www.grid.ac/institutes/grid.6190.e", 
          "name": [
            "Botanisches Institut, Universit\u00e4t zu K\u00f6ln, Gyrhofstr. 15, D-50923 K\u00f6ln, Germany, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nawrath", 
        "givenName": "Kerstin", 
        "id": "sg:person.01333546066.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01333546066.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Cologne", 
          "id": "https://www.grid.ac/institutes/grid.6190.e", 
          "name": [
            "Botanisches Institut, Universit\u00e4t zu K\u00f6ln, Gyrhofstr. 15, D-50923 K\u00f6ln, Germany, DE"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bothe", 
        "givenName": "Hermann", 
        "id": "sg:person.01324264512.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324264512.36"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1998-10", 
    "datePublishedReg": "1998-10-01", 
    "description": "The heterotrophic nitrifier Pseudomonas putida aerobically oxidized ammonia to hydroxylamine, nitrite, and nitrate. Product formation was accompanied by a small but significant release of NO, whereas N2O evolution could not be detected under the assay conditions employed. The isolate reduced nitrate to nitrite and partially further to NO under anaerobic conditions. Aerobically grown cells utilized gamma-aminobutyrate as a carbon source and as a N-source by ammonification. The physiological experiments, in particular the inhibition pattern by C2H2, indicated that P. putida expressed an ammonia monooxigenase. DNA-hybridization with an amoA gene probe coding for the smaller subunit of the ammonia monooxigenase of Nitrosomonas europaea allowed us to identify, to clone, and to sequence a region with an open reading frame showing distinct sequence similarities to the amoA gene of autotrophic ammonia oxidizers.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s002849900379", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1088675", 
        "issn": [
          "0343-8651", 
          "1432-0991"
        ], 
        "name": "Current Microbiology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "37"
      }
    ], 
    "name": "Physiological and Molecular Biological Characterization of Ammonia Oxidation of the Heterotrophic Nitrifier Pseudomonas putida", 
    "pagination": "281-288", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "a360682850e0fe52ad07d18fa79acc01cb81ef7561ace089462f3c3d0dc49f44"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "9732537"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "7808448"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s002849900379"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1034296882"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s002849900379", 
      "https://app.dimensions.ai/details/publication/pub.1034296882"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T02:01", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000514.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs002849900379"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002849900379'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002849900379'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002849900379'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002849900379'


 

This table displays all metadata directly associated to this object as RDF triples.

165 TRIPLES      20 PREDICATES      43 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s002849900379 schema:about N02be662711fc43719cb21ad906182ef1
2 N064a4d2ec0f34e27b085684b79799373
3 N0b26bcc229164f2faecd7020a60afd5c
4 N527e051c537d4bb4b6d8be0b5a0b5fa3
5 N753ce4fd58514eefa2820ac582c03886
6 N88435859602c470180063f262d031b93
7 N99987748b38749da994279402c7a8610
8 Na6bfd04d3a804264ac4fa007d3627dd3
9 Naa7bf29ec0df4ea0a1161fde41d512c9
10 Nab90dc1a3b9c4b4194140bbe5c3f7581
11 Ncc4a9ed206c246d293806abe9baba03c
12 Ndda325e0bfed4689ac40e5c48a8c4fda
13 Nf42c725a7f1e4d329aa1dbb0b8dab5e2
14 Nf6adf0cfa87d4e5bb954d920c26526e8
15 anzsrc-for:06
16 anzsrc-for:0604
17 schema:author N698b988c6737482898aeaa07767315f2
18 schema:datePublished 1998-10
19 schema:datePublishedReg 1998-10-01
20 schema:description The heterotrophic nitrifier Pseudomonas putida aerobically oxidized ammonia to hydroxylamine, nitrite, and nitrate. Product formation was accompanied by a small but significant release of NO, whereas N2O evolution could not be detected under the assay conditions employed. The isolate reduced nitrate to nitrite and partially further to NO under anaerobic conditions. Aerobically grown cells utilized gamma-aminobutyrate as a carbon source and as a N-source by ammonification. The physiological experiments, in particular the inhibition pattern by C2H2, indicated that P. putida expressed an ammonia monooxigenase. DNA-hybridization with an amoA gene probe coding for the smaller subunit of the ammonia monooxigenase of Nitrosomonas europaea allowed us to identify, to clone, and to sequence a region with an open reading frame showing distinct sequence similarities to the amoA gene of autotrophic ammonia oxidizers.
21 schema:genre research_article
22 schema:inLanguage en
23 schema:isAccessibleForFree false
24 schema:isPartOf N845303da7d004bf9b80f901366c7ccdc
25 Ndf489cb37bc644a5bc2d4c3edd838bfb
26 sg:journal.1088675
27 schema:name Physiological and Molecular Biological Characterization of Ammonia Oxidation of the Heterotrophic Nitrifier Pseudomonas putida
28 schema:pagination 281-288
29 schema:productId N005ab682c1014b069194a6d33e29c91f
30 N1bd712924fb14c5b8091e1ef32d78de2
31 N4485561441ec4286baa0302eee7aeb38
32 Na412f38971fd43d39caf69e487dfd9ee
33 Nd45f8b68282d425e88d3ffb795983a8c
34 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034296882
35 https://doi.org/10.1007/s002849900379
36 schema:sdDatePublished 2019-04-11T02:01
37 schema:sdLicense https://scigraph.springernature.com/explorer/license/
38 schema:sdPublisher N71a015eff7164a10948faee07c55c56c
39 schema:url http://link.springer.com/10.1007%2Fs002849900379
40 sgo:license sg:explorer/license/
41 sgo:sdDataset articles
42 rdf:type schema:ScholarlyArticle
43 N005ab682c1014b069194a6d33e29c91f schema:name doi
44 schema:value 10.1007/s002849900379
45 rdf:type schema:PropertyValue
46 N02be662711fc43719cb21ad906182ef1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
47 schema:name Open Reading Frames
48 rdf:type schema:DefinedTerm
49 N064a4d2ec0f34e27b085684b79799373 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
50 schema:name Nitrates
51 rdf:type schema:DefinedTerm
52 N0b26bcc229164f2faecd7020a60afd5c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
53 schema:name Amino Acid Sequence
54 rdf:type schema:DefinedTerm
55 N17960882b52040debc98b2a1863acec1 rdf:first sg:person.01265432666.28
56 rdf:rest N3b73542ff3c944759beb9eeb8544d80e
57 N19df29008c8e4b4994d140a7ce69470f rdf:first sg:person.01105672124.50
58 rdf:rest Nf38970da09e54ed2b670cb1778860999
59 N1bd712924fb14c5b8091e1ef32d78de2 schema:name nlm_unique_id
60 schema:value 7808448
61 rdf:type schema:PropertyValue
62 N3b73542ff3c944759beb9eeb8544d80e rdf:first sg:person.01333546066.23
63 rdf:rest N90c90da24834441ca888fe94ce2807ff
64 N40a6b8f421dd4237b0829038de4b532d schema:name Fraunhofer Institut für Atmosphärische Umweltforschung, Kreuzeckbahnstr. 19, D-82467 Garmisch-Partenkirchen, Germany, DE
65 rdf:type schema:Organization
66 N4485561441ec4286baa0302eee7aeb38 schema:name pubmed_id
67 schema:value 9732537
68 rdf:type schema:PropertyValue
69 N527e051c537d4bb4b6d8be0b5a0b5fa3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name Sequence Homology, Amino Acid
71 rdf:type schema:DefinedTerm
72 N698b988c6737482898aeaa07767315f2 rdf:first Ne564adb8c580495bbf741d2bc94119d9
73 rdf:rest N19df29008c8e4b4994d140a7ce69470f
74 N6e9de9996a1446d8a0ce06962c324c72 schema:name Fraunhofer Institut für Atmosphärische Umweltforschung, Kreuzeckbahnstr. 19, D-82467 Garmisch-Partenkirchen, Germany, DE
75 rdf:type schema:Organization
76 N71a015eff7164a10948faee07c55c56c schema:name Springer Nature - SN SciGraph project
77 rdf:type schema:Organization
78 N753ce4fd58514eefa2820ac582c03886 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Aerobiosis
80 rdf:type schema:DefinedTerm
81 N845303da7d004bf9b80f901366c7ccdc schema:volumeNumber 37
82 rdf:type schema:PublicationVolume
83 N88435859602c470180063f262d031b93 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Oxidation-Reduction
85 rdf:type schema:DefinedTerm
86 N90c90da24834441ca888fe94ce2807ff rdf:first sg:person.01324264512.36
87 rdf:rest rdf:nil
88 N99987748b38749da994279402c7a8610 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Hydroxylamine
90 rdf:type schema:DefinedTerm
91 Na412f38971fd43d39caf69e487dfd9ee schema:name readcube_id
92 schema:value a360682850e0fe52ad07d18fa79acc01cb81ef7561ace089462f3c3d0dc49f44
93 rdf:type schema:PropertyValue
94 Na6bfd04d3a804264ac4fa007d3627dd3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Restriction Mapping
96 rdf:type schema:DefinedTerm
97 Naa7bf29ec0df4ea0a1161fde41d512c9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Cloning, Molecular
99 rdf:type schema:DefinedTerm
100 Nab90dc1a3b9c4b4194140bbe5c3f7581 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Genes, Bacterial
102 rdf:type schema:DefinedTerm
103 Ncc4a9ed206c246d293806abe9baba03c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Ammonia
105 rdf:type schema:DefinedTerm
106 Nd45f8b68282d425e88d3ffb795983a8c schema:name dimensions_id
107 schema:value pub.1034296882
108 rdf:type schema:PropertyValue
109 Ndda325e0bfed4689ac40e5c48a8c4fda schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Molecular Sequence Data
111 rdf:type schema:DefinedTerm
112 Ndf489cb37bc644a5bc2d4c3edd838bfb schema:issueNumber 4
113 rdf:type schema:PublicationIssue
114 Ne564adb8c580495bbf741d2bc94119d9 schema:affiliation N40a6b8f421dd4237b0829038de4b532d
115 schema:familyName Daum
116 schema:givenName Michael
117 rdf:type schema:Person
118 Ne7e4d48ccb7648f88d6d1b91508abe3c schema:name Fraunhofer Institut für Atmosphärische Umweltforschung, Kreuzeckbahnstr. 19, D-82467 Garmisch-Partenkirchen, Germany, DE
119 rdf:type schema:Organization
120 Nf38970da09e54ed2b670cb1778860999 rdf:first sg:person.012142240251.29
121 rdf:rest N17960882b52040debc98b2a1863acec1
122 Nf42c725a7f1e4d329aa1dbb0b8dab5e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Pseudomonas putida
124 rdf:type schema:DefinedTerm
125 Nf6adf0cfa87d4e5bb954d920c26526e8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Oxidoreductases
127 rdf:type schema:DefinedTerm
128 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
129 schema:name Biological Sciences
130 rdf:type schema:DefinedTerm
131 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
132 schema:name Genetics
133 rdf:type schema:DefinedTerm
134 sg:journal.1088675 schema:issn 0343-8651
135 1432-0991
136 schema:name Current Microbiology
137 rdf:type schema:Periodical
138 sg:person.01105672124.50 schema:affiliation N6e9de9996a1446d8a0ce06962c324c72
139 schema:familyName Zimmer
140 schema:givenName Wolfgang
141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105672124.50
142 rdf:type schema:Person
143 sg:person.012142240251.29 schema:affiliation Ne7e4d48ccb7648f88d6d1b91508abe3c
144 schema:familyName Papen
145 schema:givenName Hans
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012142240251.29
147 rdf:type schema:Person
148 sg:person.01265432666.28 schema:affiliation https://www.grid.ac/institutes/grid.6190.e
149 schema:familyName Kloos
150 schema:givenName Karin
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265432666.28
152 rdf:type schema:Person
153 sg:person.01324264512.36 schema:affiliation https://www.grid.ac/institutes/grid.6190.e
154 schema:familyName Bothe
155 schema:givenName Hermann
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324264512.36
157 rdf:type schema:Person
158 sg:person.01333546066.23 schema:affiliation https://www.grid.ac/institutes/grid.6190.e
159 schema:familyName Nawrath
160 schema:givenName Kerstin
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01333546066.23
162 rdf:type schema:Person
163 https://www.grid.ac/institutes/grid.6190.e schema:alternateName University of Cologne
164 schema:name Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, D-50923 Köln, Germany, DE
165 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...