Volatile Ketone Formation in Bacteria: Release of 3-Oxopentanoate by Soil Pseudomonads During Growth on Heptanoate View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-04

AUTHORS

Michael G. Matiasek, Keka Choudhury, Michele Nemecek-Marshall, Ray Fall

ABSTRACT

During enrichments to search for soil bacteria that form the volatile ketones, acetone and butanone, we isolated pure cultures of Pseudomonas aureofaciens, P. fluorescens, and P. putida that excrete the beta-keto-acid, 3-oxopentanoate (3-OPA), when grown on heptanoic acid as carbon source. Analysis of 3-OPA used enzymatic decarboxylation by acetoacetate decarboxylase to yield butanone, which was detected by headspace gas chromatography. The formation of 3-OPA was strongly dependent on heptanoic acid concentration, the level of oxygen, and the state of growth, and was not seen with even-chain or other odd-chain fatty acids. Uptake of 3-OPA during stationary phase of growth is probably related to polyhydroxyalkanoate (PHA) formation in these isolates. A model for formation and release of 3-OPA is proposed. More... »

PAGES

276-281

Journal

TITLE

Current Microbiology

ISSUE

4

VOLUME

42

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s002840110217

    DOI

    http://dx.doi.org/10.1007/s002840110217

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1011784385

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/11178729


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