Volatile Ketone Formation in Bacteria: Release of 3-Oxopentanoate by Soil Pseudomonads During Growth on Heptanoate View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-04

AUTHORS

Michael G. Matiasek, Keka Choudhury, Michele Nemecek-Marshall, Ray Fall

ABSTRACT

During enrichments to search for soil bacteria that form the volatile ketones, acetone and butanone, we isolated pure cultures of Pseudomonas aureofaciens, P. fluorescens, and P. putida that excrete the beta-keto-acid, 3-oxopentanoate (3-OPA), when grown on heptanoic acid as carbon source. Analysis of 3-OPA used enzymatic decarboxylation by acetoacetate decarboxylase to yield butanone, which was detected by headspace gas chromatography. The formation of 3-OPA was strongly dependent on heptanoic acid concentration, the level of oxygen, and the state of growth, and was not seen with even-chain or other odd-chain fatty acids. Uptake of 3-OPA during stationary phase of growth is probably related to polyhydroxyalkanoate (PHA) formation in these isolates. A model for formation and release of 3-OPA is proposed. More... »

PAGES

276-281

Journal

TITLE

Current Microbiology

ISSUE

4

VOLUME

42

Author Affiliations

Related Patents

  • Microorganisms And Methods For The Co-Production Of Isopropanol And 1,4-Butanediol
  • Microorganisms And Methods For The Biosynthesis Of Aromatics, 2,4-Pentadienoate And 1,3-Butadiene
  • Microorganisms For The Production Of Methacrylic Acid
  • Microorganisms And Methods For Conversion Of Syngas And Other Carbon Sources To Useful Products
  • Methods And Organisms For Converting Synthesis Gas Or Other Gaseous Carbon Sources And Methanol To 1,3-Butanediol
  • Semi-Synthetic Terephthalic Acid Via Microorganisms That Produce Muconic Acid
  • Microorganisms And Methods For The Biosynthesis Of Adipate, Hexamethylenediamine And 6-Aminocaproic Acid
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms And Methods For The Biosynthesis Of Butadiene
  • Microorganisms And Methods For The Biosynthesis Of Propylene
  • Semi-Synthetic Terephthalic Acid Via Microorganisms That Produce Muconic Acid
  • Microorganisms And Methods For The Biosynthesis Of Adipate, Hexamethylenediamine And 6-Aminocaproic Acid
  • Microorganisms And Methods For The Production Of Caprolactone
  • Methods For The Synthesis Of Olefins And Derivatives
  • Microorganisms And Methods For Carbon-Efficient Biosynthesis Of Mek And 2-Butanol
  • Microorganisms And Methods For Producing Alkenes
  • Microorganisms And Methods For The Biosynthesis Of Fumarate, Malate, And Acrylate
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms And Methods For The Biosynthesis Of Aromatics, 2,4-Pentadienoate And 1,3-Butadiene
  • Microorganisms And Methods For The Production Of Caprolactone
  • Organisms For The Production Of 1,3-Butanediol
  • Organisms For The Production Of 1,3-Butanediol
  • Microbial Organisms Comprising Exogenous Nucleic Acids Encoding Reductive Tca Pathway Enzymes
  • Microorganisms And Methods For The Production Of Caprolactone
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Primary Alcohol Producing Organisms
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Methods For Increasing Product Yields
  • Microorganisms For The Production Of 1,4-Butanediol, 4-Hydroxybutanal, 4-Hydroxybutyryl-Coa, Putrescine And Related Compounds, And Methods Related Thereto
  • Organisms For The Production Of Isopropanol, N-Butanol, And Isobutanol
  • Primary Alcohol Producing Organisms
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s002840110217

    DOI

    http://dx.doi.org/10.1007/s002840110217

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1011784385

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/11178729


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0503", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Soil Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/05", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Environmental Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Acetone", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Butanones", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Carboxy-Lyases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chromatography, Gas", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Heptanoic Acids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Oxygen", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pentanoic Acids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pseudomonas", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Soil Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Substrate Specificity", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Colorado Boulder", 
              "id": "https://www.grid.ac/institutes/grid.266190.a", 
              "name": [
                "Department of Chemistry and Biochemistry, Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, CO 80309-0215, USA, US"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Matiasek", 
            "givenName": "Michael G.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Colorado Boulder", 
              "id": "https://www.grid.ac/institutes/grid.266190.a", 
              "name": [
                "Department of Chemistry and Biochemistry, Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, CO 80309-0215, USA, US"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Choudhury", 
            "givenName": "Keka", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Colorado Boulder", 
              "id": "https://www.grid.ac/institutes/grid.266190.a", 
              "name": [
                "Department of Chemistry and Biochemistry, Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, CO 80309-0215, USA, US"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nemecek-Marshall", 
            "givenName": "Michele", 
            "id": "sg:person.01327613674.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327613674.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Colorado Boulder", 
              "id": "https://www.grid.ac/institutes/grid.266190.a", 
              "name": [
                "Department of Chemistry and Biochemistry, Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, CO 80309-0215, USA, US"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Fall", 
            "givenName": "Ray", 
            "id": "sg:person.01117314472.64", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01117314472.64"
            ], 
            "type": "Person"
          }
        ], 
        "datePublished": "2001-04", 
        "datePublishedReg": "2001-04-01", 
        "description": "During enrichments to search for soil bacteria that form the volatile ketones, acetone and butanone, we isolated pure cultures of Pseudomonas aureofaciens, P. fluorescens, and P. putida that excrete the beta-keto-acid, 3-oxopentanoate (3-OPA), when grown on heptanoic acid as carbon source. Analysis of 3-OPA used enzymatic decarboxylation by acetoacetate decarboxylase to yield butanone, which was detected by headspace gas chromatography. The formation of 3-OPA was strongly dependent on heptanoic acid concentration, the level of oxygen, and the state of growth, and was not seen with even-chain or other odd-chain fatty acids. Uptake of 3-OPA during stationary phase of growth is probably related to polyhydroxyalkanoate (PHA) formation in these isolates. A model for formation and release of 3-OPA is proposed.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s002840110217", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1088675", 
            "issn": [
              "0343-8651", 
              "1432-0991"
            ], 
            "name": "Current Microbiology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "4", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "42"
          }
        ], 
        "name": "Volatile Ketone Formation in Bacteria: Release of 3-Oxopentanoate by Soil Pseudomonads During Growth on Heptanoate", 
        "pagination": "276-281", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "3825bb56ae6b79fa17d38d9e4a256f02b44d53337e022c2e9f7e5f9fea3a9200"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "11178729"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "7808448"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s002840110217"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1011784385"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s002840110217", 
          "https://app.dimensions.ai/details/publication/pub.1011784385"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T16:42", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000511.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs002840110217"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002840110217'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002840110217'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002840110217'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002840110217'


     

    This table displays all metadata directly associated to this object as RDF triples.

    128 TRIPLES      20 PREDICATES      39 URIs      31 LITERALS      19 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s002840110217 schema:about N4bc130048feb41a89aa2fc713e747744
    2 N4eaa43d5f60f4d128cc65964aed179e7
    3 N599753c9f4a444caa23c6f98a67ceedd
    4 N6b48b7984ab14967ad374493223b67a6
    5 N7acb1238e4f94b7aa6de12eba745ce1a
    6 N7fe2754e632d4add85b5758bb73d567e
    7 N824e72c243ca438f9866bd036b5986ff
    8 Na502645be55e4ebfa8eeaad60ef30516
    9 Nbe8e82f57f464c2d981a445916e4b50a
    10 Nca28493e1cd542c2b6deaa468bf450c0
    11 anzsrc-for:05
    12 anzsrc-for:0503
    13 schema:author Na3590ef5780e42f68800bcf4f9215d82
    14 schema:datePublished 2001-04
    15 schema:datePublishedReg 2001-04-01
    16 schema:description During enrichments to search for soil bacteria that form the volatile ketones, acetone and butanone, we isolated pure cultures of Pseudomonas aureofaciens, P. fluorescens, and P. putida that excrete the beta-keto-acid, 3-oxopentanoate (3-OPA), when grown on heptanoic acid as carbon source. Analysis of 3-OPA used enzymatic decarboxylation by acetoacetate decarboxylase to yield butanone, which was detected by headspace gas chromatography. The formation of 3-OPA was strongly dependent on heptanoic acid concentration, the level of oxygen, and the state of growth, and was not seen with even-chain or other odd-chain fatty acids. Uptake of 3-OPA during stationary phase of growth is probably related to polyhydroxyalkanoate (PHA) formation in these isolates. A model for formation and release of 3-OPA is proposed.
    17 schema:genre research_article
    18 schema:inLanguage en
    19 schema:isAccessibleForFree false
    20 schema:isPartOf N9099e59b28b3410298c3cbeaae64b4f3
    21 Ne92b283819e04b569408057d672c9b8c
    22 sg:journal.1088675
    23 schema:name Volatile Ketone Formation in Bacteria: Release of 3-Oxopentanoate by Soil Pseudomonads During Growth on Heptanoate
    24 schema:pagination 276-281
    25 schema:productId N08ca2c0f28604616b064fb523e900d7b
    26 N0d710164e0954994aeac1f39f0968b34
    27 N4856ee3ea134479faf80f16be0d0461c
    28 N8c426e7b7beb4564873603778abd50cb
    29 Nd3163f67bf5b46a9bf06a7866ea2c076
    30 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011784385
    31 https://doi.org/10.1007/s002840110217
    32 schema:sdDatePublished 2019-04-10T16:42
    33 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    34 schema:sdPublisher N207e20d6cdf040938ac73810f4dae13b
    35 schema:url http://link.springer.com/10.1007%2Fs002840110217
    36 sgo:license sg:explorer/license/
    37 sgo:sdDataset articles
    38 rdf:type schema:ScholarlyArticle
    39 N08ca2c0f28604616b064fb523e900d7b schema:name readcube_id
    40 schema:value 3825bb56ae6b79fa17d38d9e4a256f02b44d53337e022c2e9f7e5f9fea3a9200
    41 rdf:type schema:PropertyValue
    42 N0d710164e0954994aeac1f39f0968b34 schema:name dimensions_id
    43 schema:value pub.1011784385
    44 rdf:type schema:PropertyValue
    45 N207e20d6cdf040938ac73810f4dae13b schema:name Springer Nature - SN SciGraph project
    46 rdf:type schema:Organization
    47 N2b90a7db96c249c5978715ec2e2f6146 rdf:first sg:person.01117314472.64
    48 rdf:rest rdf:nil
    49 N4856ee3ea134479faf80f16be0d0461c schema:name nlm_unique_id
    50 schema:value 7808448
    51 rdf:type schema:PropertyValue
    52 N4bc130048feb41a89aa2fc713e747744 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    53 schema:name Carboxy-Lyases
    54 rdf:type schema:DefinedTerm
    55 N4eaa43d5f60f4d128cc65964aed179e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    56 schema:name Chromatography, Gas
    57 rdf:type schema:DefinedTerm
    58 N599753c9f4a444caa23c6f98a67ceedd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    59 schema:name Butanones
    60 rdf:type schema:DefinedTerm
    61 N6322be40a8314a179e1ab7e5e0bda184 schema:affiliation https://www.grid.ac/institutes/grid.266190.a
    62 schema:familyName Matiasek
    63 schema:givenName Michael G.
    64 rdf:type schema:Person
    65 N6b48b7984ab14967ad374493223b67a6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    66 schema:name Acetone
    67 rdf:type schema:DefinedTerm
    68 N7189cc7f9d724896b96d5207adce181b rdf:first Nf441054ab15d4888987d1b251fa3c0a7
    69 rdf:rest N86108988d0254fb6a588f7be231b85e6
    70 N7acb1238e4f94b7aa6de12eba745ce1a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    71 schema:name Heptanoic Acids
    72 rdf:type schema:DefinedTerm
    73 N7fe2754e632d4add85b5758bb73d567e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    74 schema:name Pseudomonas
    75 rdf:type schema:DefinedTerm
    76 N824e72c243ca438f9866bd036b5986ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    77 schema:name Soil Microbiology
    78 rdf:type schema:DefinedTerm
    79 N86108988d0254fb6a588f7be231b85e6 rdf:first sg:person.01327613674.26
    80 rdf:rest N2b90a7db96c249c5978715ec2e2f6146
    81 N8c426e7b7beb4564873603778abd50cb schema:name doi
    82 schema:value 10.1007/s002840110217
    83 rdf:type schema:PropertyValue
    84 N9099e59b28b3410298c3cbeaae64b4f3 schema:issueNumber 4
    85 rdf:type schema:PublicationIssue
    86 Na3590ef5780e42f68800bcf4f9215d82 rdf:first N6322be40a8314a179e1ab7e5e0bda184
    87 rdf:rest N7189cc7f9d724896b96d5207adce181b
    88 Na502645be55e4ebfa8eeaad60ef30516 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    89 schema:name Pentanoic Acids
    90 rdf:type schema:DefinedTerm
    91 Nbe8e82f57f464c2d981a445916e4b50a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    92 schema:name Oxygen
    93 rdf:type schema:DefinedTerm
    94 Nca28493e1cd542c2b6deaa468bf450c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    95 schema:name Substrate Specificity
    96 rdf:type schema:DefinedTerm
    97 Nd3163f67bf5b46a9bf06a7866ea2c076 schema:name pubmed_id
    98 schema:value 11178729
    99 rdf:type schema:PropertyValue
    100 Ne92b283819e04b569408057d672c9b8c schema:volumeNumber 42
    101 rdf:type schema:PublicationVolume
    102 Nf441054ab15d4888987d1b251fa3c0a7 schema:affiliation https://www.grid.ac/institutes/grid.266190.a
    103 schema:familyName Choudhury
    104 schema:givenName Keka
    105 rdf:type schema:Person
    106 anzsrc-for:05 schema:inDefinedTermSet anzsrc-for:
    107 schema:name Environmental Sciences
    108 rdf:type schema:DefinedTerm
    109 anzsrc-for:0503 schema:inDefinedTermSet anzsrc-for:
    110 schema:name Soil Sciences
    111 rdf:type schema:DefinedTerm
    112 sg:journal.1088675 schema:issn 0343-8651
    113 1432-0991
    114 schema:name Current Microbiology
    115 rdf:type schema:Periodical
    116 sg:person.01117314472.64 schema:affiliation https://www.grid.ac/institutes/grid.266190.a
    117 schema:familyName Fall
    118 schema:givenName Ray
    119 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01117314472.64
    120 rdf:type schema:Person
    121 sg:person.01327613674.26 schema:affiliation https://www.grid.ac/institutes/grid.266190.a
    122 schema:familyName Nemecek-Marshall
    123 schema:givenName Michele
    124 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327613674.26
    125 rdf:type schema:Person
    126 https://www.grid.ac/institutes/grid.266190.a schema:alternateName University of Colorado Boulder
    127 schema:name Department of Chemistry and Biochemistry, Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, CO 80309-0215, USA, US
    128 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...