Transformation of Internal Extracellular Bacteria Isolated from Rhagoletis completa Cresson Gut with Enhanced Green Fluorescent Protein View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-06

AUTHORS

John J. Peloquin, Lyudmila Kuzina, Carol R. Lauzon, Thomas A. Miller

ABSTRACT

We discovered Zeocintrade mark is an effective antibiotic against Enterobacter agglomerans and Klebsiella pneumoniae strains isolated from the walnut husk fly (Rhagoletis completa Cresson: Family Tephritidae) and that bleomycin resistance can be used as a selective marker in transforming plasmids. We transformed Ent. agglomerans and K. pneumoniae strains originally isolated from their close association with R. completa gut to produce enhanced green fluorescent protein, a variant of green fluorescent protein in the first demonstration of genetic transformation of internal extracellular bacteria isolated from a tephritid pest. We report methods for plasmid-mediated transformation of these bacteria, the expression of fluorescent marker protein from the transforming plasmids, and the stability of the transforming plasmid in the bacteria. We also discuss applications of this technology in the study of pest biology and control implementation. More... »

PAGES

367-371

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002840010072

DOI

http://dx.doi.org/10.1007/s002840010072

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036542658

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10827278


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Anti-Bacterial Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bleomycin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Diptera", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Resistance, Microbial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electrophoresis, Agar Gel", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electroporation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enterobacteriaceae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Markers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Green Fluorescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Luminescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Time Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transformation, Bacterial", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of California, Riverside", 
          "id": "https://www.grid.ac/institutes/grid.266097.c", 
          "name": [
            "Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA, US"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Peloquin", 
        "givenName": "John J.", 
        "id": "sg:person.0700133621.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700133621.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, Riverside", 
          "id": "https://www.grid.ac/institutes/grid.266097.c", 
          "name": [
            "Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA, US"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kuzina", 
        "givenName": "Lyudmila", 
        "id": "sg:person.0746247021.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746247021.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "California State University, East Bay", 
          "id": "https://www.grid.ac/institutes/grid.253557.3", 
          "name": [
            "Department of Biological Sciences, California State University-Hayward, Hayward, CA 94542, USA, US"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lauzon", 
        "givenName": "Carol R.", 
        "id": "sg:person.01035426506.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035426506.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, Riverside", 
          "id": "https://www.grid.ac/institutes/grid.266097.c", 
          "name": [
            "Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA, US"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miller", 
        "givenName": "Thomas A.", 
        "id": "sg:person.01047000573.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047000573.48"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2000-06", 
    "datePublishedReg": "2000-06-01", 
    "description": "We discovered Zeocintrade mark is an effective antibiotic against Enterobacter agglomerans and Klebsiella pneumoniae strains isolated from the walnut husk fly (Rhagoletis completa Cresson: Family Tephritidae) and that bleomycin resistance can be used as a selective marker in transforming plasmids. We transformed Ent. agglomerans and K. pneumoniae strains originally isolated from their close association with R. completa gut to produce enhanced green fluorescent protein, a variant of green fluorescent protein in the first demonstration of genetic transformation of internal extracellular bacteria isolated from a tephritid pest. We report methods for plasmid-mediated transformation of these bacteria, the expression of fluorescent marker protein from the transforming plasmids, and the stability of the transforming plasmid in the bacteria. We also discuss applications of this technology in the study of pest biology and control implementation.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s002840010072", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1088675", 
        "issn": [
          "0343-8651", 
          "1432-0991"
        ], 
        "name": "Current Microbiology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "40"
      }
    ], 
    "name": "Transformation of Internal Extracellular Bacteria Isolated from Rhagoletis completa Cresson Gut with Enhanced Green Fluorescent Protein", 
    "pagination": "367-371", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "ae12fad7d27f0f7eb3621bc5682e0be1153cc047e45f26059c12dd6e52734777"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10827278"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "7808448"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s002840010072"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1036542658"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s002840010072", 
      "https://app.dimensions.ai/details/publication/pub.1036542658"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T14:10", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000514.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs002840010072"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002840010072'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002840010072'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002840010072'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002840010072'


 

This table displays all metadata directly associated to this object as RDF triples.

149 TRIPLES      20 PREDICATES      43 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s002840010072 schema:about N11f15e0104794d6fbee83d0ba04735bc
2 N34d7c3288070438ba9ee9b7b8556203a
3 N726441829eea4dae99e39418083c6de6
4 N8daf86866101437aa6db629cab1ab8f3
5 N9dce0c956aea45198336bc6d52b0e7bf
6 Na0096ec216054f6ab407d5bf8de0015f
7 Nab016abd5fda4825b35a8ba5935063d6
8 Nb02a5e859ef24163b34f6173fa1c7ce6
9 Nb45aa5a8c2be4ed4909fd7794377acdc
10 Nc98f20fcc7c4477b972b7f88e53f6acd
11 Nd682a416089348f999de12f8b57a16b5
12 Nd9fcab39b66948d79292bee51269a951
13 Ndd7554b6f1c543a489f6d754bdc7e994
14 Ndf028de1ee0141d39ffd312905cd9af4
15 anzsrc-for:06
16 anzsrc-for:0604
17 schema:author N44a75e36a45a41559bcf835aa70832e6
18 schema:datePublished 2000-06
19 schema:datePublishedReg 2000-06-01
20 schema:description We discovered Zeocintrade mark is an effective antibiotic against Enterobacter agglomerans and Klebsiella pneumoniae strains isolated from the walnut husk fly (Rhagoletis completa Cresson: Family Tephritidae) and that bleomycin resistance can be used as a selective marker in transforming plasmids. We transformed Ent. agglomerans and K. pneumoniae strains originally isolated from their close association with R. completa gut to produce enhanced green fluorescent protein, a variant of green fluorescent protein in the first demonstration of genetic transformation of internal extracellular bacteria isolated from a tephritid pest. We report methods for plasmid-mediated transformation of these bacteria, the expression of fluorescent marker protein from the transforming plasmids, and the stability of the transforming plasmid in the bacteria. We also discuss applications of this technology in the study of pest biology and control implementation.
21 schema:genre research_article
22 schema:inLanguage en
23 schema:isAccessibleForFree false
24 schema:isPartOf N1d283494565c4ed292220df8932e0abf
25 N29b39d00ecfc408c90593b7c01229002
26 sg:journal.1088675
27 schema:name Transformation of Internal Extracellular Bacteria Isolated from Rhagoletis completa Cresson Gut with Enhanced Green Fluorescent Protein
28 schema:pagination 367-371
29 schema:productId N7c02a85f964041f28d58d209b0c58ee3
30 N876346924e8e4bbe941897a017f0bbbe
31 Na214fbd496ed4794846fe388095c99d6
32 Nc6b05cc312f34390969a1d0312064e22
33 Ne70facbee5594d108387c027f8b799d4
34 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036542658
35 https://doi.org/10.1007/s002840010072
36 schema:sdDatePublished 2019-04-10T14:10
37 schema:sdLicense https://scigraph.springernature.com/explorer/license/
38 schema:sdPublisher Nabab177bb92c404983c1c3213b98789e
39 schema:url http://link.springer.com/10.1007%2Fs002840010072
40 sgo:license sg:explorer/license/
41 sgo:sdDataset articles
42 rdf:type schema:ScholarlyArticle
43 N11f15e0104794d6fbee83d0ba04735bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
44 schema:name Electroporation
45 rdf:type schema:DefinedTerm
46 N1d283494565c4ed292220df8932e0abf schema:issueNumber 6
47 rdf:type schema:PublicationIssue
48 N29b39d00ecfc408c90593b7c01229002 schema:volumeNumber 40
49 rdf:type schema:PublicationVolume
50 N34d7c3288070438ba9ee9b7b8556203a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
51 schema:name Animals
52 rdf:type schema:DefinedTerm
53 N44a75e36a45a41559bcf835aa70832e6 rdf:first sg:person.0700133621.07
54 rdf:rest Nfd05c4e349c14a728d527f9fbc9d5ce7
55 N726441829eea4dae99e39418083c6de6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
56 schema:name Transformation, Bacterial
57 rdf:type schema:DefinedTerm
58 N7c02a85f964041f28d58d209b0c58ee3 schema:name pubmed_id
59 schema:value 10827278
60 rdf:type schema:PropertyValue
61 N876346924e8e4bbe941897a017f0bbbe schema:name dimensions_id
62 schema:value pub.1036542658
63 rdf:type schema:PropertyValue
64 N8daf86866101437aa6db629cab1ab8f3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
65 schema:name Bleomycin
66 rdf:type schema:DefinedTerm
67 N9dce0c956aea45198336bc6d52b0e7bf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Drug Resistance, Microbial
69 rdf:type schema:DefinedTerm
70 Na0096ec216054f6ab407d5bf8de0015f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Green Fluorescent Proteins
72 rdf:type schema:DefinedTerm
73 Na214fbd496ed4794846fe388095c99d6 schema:name readcube_id
74 schema:value ae12fad7d27f0f7eb3621bc5682e0be1153cc047e45f26059c12dd6e52734777
75 rdf:type schema:PropertyValue
76 Na512c7bf32ff402e8a7165273792668b rdf:first sg:person.01035426506.26
77 rdf:rest Nf3aca6dde32e4df9ae8f4bb4add947c9
78 Nab016abd5fda4825b35a8ba5935063d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Diptera
80 rdf:type schema:DefinedTerm
81 Nabab177bb92c404983c1c3213b98789e schema:name Springer Nature - SN SciGraph project
82 rdf:type schema:Organization
83 Nb02a5e859ef24163b34f6173fa1c7ce6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Genetic Markers
85 rdf:type schema:DefinedTerm
86 Nb45aa5a8c2be4ed4909fd7794377acdc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Plasmids
88 rdf:type schema:DefinedTerm
89 Nc6b05cc312f34390969a1d0312064e22 schema:name nlm_unique_id
90 schema:value 7808448
91 rdf:type schema:PropertyValue
92 Nc98f20fcc7c4477b972b7f88e53f6acd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Luminescent Proteins
94 rdf:type schema:DefinedTerm
95 Nd682a416089348f999de12f8b57a16b5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Time Factors
97 rdf:type schema:DefinedTerm
98 Nd9fcab39b66948d79292bee51269a951 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Enterobacteriaceae
100 rdf:type schema:DefinedTerm
101 Ndd7554b6f1c543a489f6d754bdc7e994 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Anti-Bacterial Agents
103 rdf:type schema:DefinedTerm
104 Ndf028de1ee0141d39ffd312905cd9af4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Electrophoresis, Agar Gel
106 rdf:type schema:DefinedTerm
107 Ne70facbee5594d108387c027f8b799d4 schema:name doi
108 schema:value 10.1007/s002840010072
109 rdf:type schema:PropertyValue
110 Nf3aca6dde32e4df9ae8f4bb4add947c9 rdf:first sg:person.01047000573.48
111 rdf:rest rdf:nil
112 Nfd05c4e349c14a728d527f9fbc9d5ce7 rdf:first sg:person.0746247021.00
113 rdf:rest Na512c7bf32ff402e8a7165273792668b
114 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
115 schema:name Biological Sciences
116 rdf:type schema:DefinedTerm
117 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
118 schema:name Genetics
119 rdf:type schema:DefinedTerm
120 sg:journal.1088675 schema:issn 0343-8651
121 1432-0991
122 schema:name Current Microbiology
123 rdf:type schema:Periodical
124 sg:person.01035426506.26 schema:affiliation https://www.grid.ac/institutes/grid.253557.3
125 schema:familyName Lauzon
126 schema:givenName Carol R.
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035426506.26
128 rdf:type schema:Person
129 sg:person.01047000573.48 schema:affiliation https://www.grid.ac/institutes/grid.266097.c
130 schema:familyName Miller
131 schema:givenName Thomas A.
132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047000573.48
133 rdf:type schema:Person
134 sg:person.0700133621.07 schema:affiliation https://www.grid.ac/institutes/grid.266097.c
135 schema:familyName Peloquin
136 schema:givenName John J.
137 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700133621.07
138 rdf:type schema:Person
139 sg:person.0746247021.00 schema:affiliation https://www.grid.ac/institutes/grid.266097.c
140 schema:familyName Kuzina
141 schema:givenName Lyudmila
142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746247021.00
143 rdf:type schema:Person
144 https://www.grid.ac/institutes/grid.253557.3 schema:alternateName California State University, East Bay
145 schema:name Department of Biological Sciences, California State University-Hayward, Hayward, CA 94542, USA, US
146 rdf:type schema:Organization
147 https://www.grid.ac/institutes/grid.266097.c schema:alternateName University of California, Riverside
148 schema:name Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA, US
149 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...