Analysis of the Impact of Rosuvastatin on Bacterial Mevalonate Production Using a UPLC-Mass Spectrometry Approach View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-03-10

AUTHORS

J. A. Nolan, M. Kinsella, C. Hill, S. A. Joyce, C. G. M. Gahan

ABSTRACT

Statins are widely prescribed cholesterol-lowering medications and act through inhibition of the human enzyme 3-methylglutaryl coenzyme A reductase (HMG-R) which produces mevalonate (MVAL), a key substrate for cholesterol biosynthesis. Some important microbial species also express an isoform of HMG-R; however, the nature of the interaction between statins and bacteria is currently unclear and studies would benefit from protocols to quantify MVAL in complex microbial environments. The objective of this study was to develop a protocol for the analytical quantification of MVAL in bacterial systems and to utilise this approach to analyse the effects of Rosuvastatin (RSV) on bacterial MVAL formation. To determine the effective concentration range of RSV, we examined the dose-dependent inhibition of growth in the HMG-R+ bacterial pathogens Listeria monocytogenes, Staphylococcus aureus and Enterococcus faecium at various concentrations of pure RSV. Growth inhibition generally correlated with a reduction in bacterial MVAL levels, particularly in culture supernatants at high RSV concentrations, as determined using our ultra-performance liquid chromatography mass spectrometry protocol. This work therefore outlines a refined protocol for the analysis of MVAL in microbial cultures and provides evidence for statin-mediated inhibition of bacterial HMG-R. Furthermore, we show that MVAL is readily transported and secreted from bacterial cells into the growth media. More... »

PAGES

1-8

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00284-016-1014-z

DOI

http://dx.doi.org/10.1007/s00284-016-1014-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046418218

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26960292


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatography, High Pressure Liquid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enterococcus faecium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Listeria monocytogenes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mass Spectrometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mevalonic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rosuvastatin Calcium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Staphylococcus aureus", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "School of Microbiology, University College Cork, Cork, Ireland", 
          "id": "http://www.grid.ac/institutes/grid.7872.a", 
          "name": [
            "APC Microbiome Institute, University College Cork, Cork, Ireland", 
            "School of Microbiology, University College Cork, Cork, Ireland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nolan", 
        "givenName": "J. A.", 
        "id": "sg:person.0775107227.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0775107227.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Science, School of Science & Computing, Waterford Institute of Technology, Waterford, Ireland", 
          "id": "http://www.grid.ac/institutes/grid.24349.38", 
          "name": [
            "School of Food and Nutritional Sciences, University College Cork, Cork, Ireland", 
            "Department of Science, School of Science & Computing, Waterford Institute of Technology, Waterford, Ireland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kinsella", 
        "givenName": "M.", 
        "id": "sg:person.01161643776.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01161643776.94"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Microbiology, University College Cork, Cork, Ireland", 
          "id": "http://www.grid.ac/institutes/grid.7872.a", 
          "name": [
            "APC Microbiome Institute, University College Cork, Cork, Ireland", 
            "School of Microbiology, University College Cork, Cork, Ireland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hill", 
        "givenName": "C.", 
        "id": "sg:person.01202224511.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01202224511.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Biochemistry & Cell Biology, University College Cork, Cork, Ireland", 
          "id": "http://www.grid.ac/institutes/grid.7872.a", 
          "name": [
            "APC Microbiome Institute, University College Cork, Cork, Ireland", 
            "School of Biochemistry & Cell Biology, University College Cork, Cork, Ireland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Joyce", 
        "givenName": "S. A.", 
        "id": "sg:person.0605260211.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605260211.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Pharmacy, University College Cork, Cork, Ireland", 
          "id": "http://www.grid.ac/institutes/grid.7872.a", 
          "name": [
            "APC Microbiome Institute, University College Cork, Cork, Ireland", 
            "School of Microbiology, University College Cork, Cork, Ireland", 
            "School of Pharmacy, University College Cork, Cork, Ireland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gahan", 
        "givenName": "C. G. M.", 
        "id": "sg:person.014255126037.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014255126037.78"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nbt.1557", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022462669", 
          "https://doi.org/10.1038/nbt.1557"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ja.2012.95", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008567184", 
          "https://doi.org/10.1038/ja.2012.95"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1476-0711-11-13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040573060", 
          "https://doi.org/10.1186/1476-0711-11-13"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-03-10", 
    "datePublishedReg": "2016-03-10", 
    "description": "Statins are widely prescribed cholesterol-lowering medications and act through inhibition of the human enzyme 3-methylglutaryl coenzyme A reductase (HMG-R) which produces mevalonate (MVAL), a key substrate for cholesterol biosynthesis. Some important microbial species also express an isoform of HMG-R; however, the nature of the interaction between statins and bacteria is currently unclear and studies would benefit from protocols to quantify MVAL in complex microbial environments. The objective of this study was to develop a protocol for the analytical quantification of MVAL in bacterial systems and to utilise this approach to analyse the effects of Rosuvastatin (RSV) on bacterial MVAL formation. To determine the effective concentration range of RSV, we examined the dose-dependent inhibition of growth in the HMG-R+ bacterial pathogens Listeria monocytogenes, Staphylococcus aureus and Enterococcus faecium at various concentrations of pure RSV. Growth inhibition generally correlated with a reduction in bacterial MVAL levels, particularly in culture supernatants at high RSV concentrations, as determined using our ultra-performance liquid chromatography mass spectrometry protocol. This work therefore outlines a refined protocol for the analysis of MVAL in microbial cultures and provides evidence for statin-mediated inhibition of bacterial HMG-R. Furthermore, we show that MVAL is readily transported and secreted from bacterial cells into the growth media.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s00284-016-1014-z", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3984108", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1088675", 
        "issn": [
          "0343-8651", 
          "1432-0991"
        ], 
        "name": "Current Microbiology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "73"
      }
    ], 
    "keywords": [
      "bacterial pathogen Listeria monocytogenes", 
      "important microbial species", 
      "complex microbial environments", 
      "pathogen Listeria monocytogenes", 
      "microbial species", 
      "bacterial systems", 
      "bacterial cells", 
      "key substrate", 
      "microbial environment", 
      "growth medium", 
      "mevalonate production", 
      "cholesterol biosynthesis", 
      "spectrometry approach", 
      "microbial cultures", 
      "growth inhibition", 
      "mass spectrometry protocol", 
      "cholesterol-lowering medications", 
      "dose-dependent inhibition", 
      "impact of rosuvastatin", 
      "effect of rosuvastatin", 
      "Listeria monocytogenes", 
      "culture supernatants", 
      "inhibition", 
      "HMG", 
      "biosynthesis", 
      "refined protocol", 
      "reductase", 
      "species", 
      "isoforms", 
      "bacteria", 
      "effective concentration range", 
      "rosuvastatin", 
      "statins", 
      "coenzyme", 
      "Enterococcus faecium", 
      "cells", 
      "Staphylococcus aureus", 
      "RSV concentrations", 
      "growth", 
      "MVAL", 
      "monocytogenes", 
      "substrate", 
      "production", 
      "supernatant", 
      "culture", 
      "medications", 
      "interaction", 
      "analysis", 
      "formation", 
      "faecium", 
      "protocol", 
      "study", 
      "analytical quantification", 
      "medium", 
      "aureus", 
      "concentration", 
      "evidence", 
      "levels", 
      "environment", 
      "quantification", 
      "reduction", 
      "effect", 
      "approach", 
      "objective", 
      "range", 
      "impact", 
      "nature", 
      "system", 
      "concentration range", 
      "work", 
      "human enzyme 3-methylglutaryl coenzyme", 
      "enzyme 3-methylglutaryl coenzyme", 
      "isoform of HMG", 
      "bacterial MVAL formation", 
      "MVAL formation", 
      "pure RSV", 
      "bacterial MVAL levels", 
      "MVAL levels", 
      "high RSV concentrations", 
      "ultra-performance liquid chromatography mass spectrometry protocol", 
      "liquid chromatography mass spectrometry protocol", 
      "chromatography mass spectrometry protocol", 
      "spectrometry protocol", 
      "analysis of MVAL", 
      "bacterial HMG", 
      "Bacterial Mevalonate Production", 
      "UPLC-Mass Spectrometry Approach"
    ], 
    "name": "Analysis of the Impact of Rosuvastatin on Bacterial Mevalonate Production Using a UPLC-Mass Spectrometry Approach", 
    "pagination": "1-8", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1046418218"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00284-016-1014-z"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "26960292"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00284-016-1014-z", 
      "https://app.dimensions.ai/details/publication/pub.1046418218"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-11-01T18:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211101/entities/gbq_results/article/article_703.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s00284-016-1014-z"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00284-016-1014-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00284-016-1014-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00284-016-1014-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00284-016-1014-z'


 

This table displays all metadata directly associated to this object as RDF triples.

228 TRIPLES      22 PREDICATES      123 URIs      112 LITERALS      14 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00284-016-1014-z schema:about N2699fe496d46463eae37831d061399e5
2 N807d8bebc9bb4a0bbce98384de5ad32c
3 Nbd6333b15c804487a3536916d47e28e7
4 Nd046ba8fceda45adafc6569d73feb93b
5 Ne0786e3b63964c80aafe2564c12e05b8
6 Ne96f4b702e3841dfb776c51296666acd
7 Neb86797453ae452cbd56b5071527d5e3
8 anzsrc-for:06
9 anzsrc-for:0605
10 schema:author N1bd25247b2334ddb84ca12b43ad9783c
11 schema:citation sg:pub.10.1038/ja.2012.95
12 sg:pub.10.1038/nbt.1557
13 sg:pub.10.1186/1476-0711-11-13
14 schema:datePublished 2016-03-10
15 schema:datePublishedReg 2016-03-10
16 schema:description Statins are widely prescribed cholesterol-lowering medications and act through inhibition of the human enzyme 3-methylglutaryl coenzyme A reductase (HMG-R) which produces mevalonate (MVAL), a key substrate for cholesterol biosynthesis. Some important microbial species also express an isoform of HMG-R; however, the nature of the interaction between statins and bacteria is currently unclear and studies would benefit from protocols to quantify MVAL in complex microbial environments. The objective of this study was to develop a protocol for the analytical quantification of MVAL in bacterial systems and to utilise this approach to analyse the effects of Rosuvastatin (RSV) on bacterial MVAL formation. To determine the effective concentration range of RSV, we examined the dose-dependent inhibition of growth in the HMG-R+ bacterial pathogens Listeria monocytogenes, Staphylococcus aureus and Enterococcus faecium at various concentrations of pure RSV. Growth inhibition generally correlated with a reduction in bacterial MVAL levels, particularly in culture supernatants at high RSV concentrations, as determined using our ultra-performance liquid chromatography mass spectrometry protocol. This work therefore outlines a refined protocol for the analysis of MVAL in microbial cultures and provides evidence for statin-mediated inhibition of bacterial HMG-R. Furthermore, we show that MVAL is readily transported and secreted from bacterial cells into the growth media.
17 schema:genre article
18 schema:inLanguage en
19 schema:isAccessibleForFree false
20 schema:isPartOf N7e1c79a0e476459a80d3ae4b3f898992
21 N9e6a86bd249d4edea1401532168b771c
22 sg:journal.1088675
23 schema:keywords Bacterial Mevalonate Production
24 Enterococcus faecium
25 HMG
26 Listeria monocytogenes
27 MVAL
28 MVAL formation
29 MVAL levels
30 RSV concentrations
31 Staphylococcus aureus
32 UPLC-Mass Spectrometry Approach
33 analysis
34 analysis of MVAL
35 analytical quantification
36 approach
37 aureus
38 bacteria
39 bacterial HMG
40 bacterial MVAL formation
41 bacterial MVAL levels
42 bacterial cells
43 bacterial pathogen Listeria monocytogenes
44 bacterial systems
45 biosynthesis
46 cells
47 cholesterol biosynthesis
48 cholesterol-lowering medications
49 chromatography mass spectrometry protocol
50 coenzyme
51 complex microbial environments
52 concentration
53 concentration range
54 culture
55 culture supernatants
56 dose-dependent inhibition
57 effect
58 effect of rosuvastatin
59 effective concentration range
60 environment
61 enzyme 3-methylglutaryl coenzyme
62 evidence
63 faecium
64 formation
65 growth
66 growth inhibition
67 growth medium
68 high RSV concentrations
69 human enzyme 3-methylglutaryl coenzyme
70 impact
71 impact of rosuvastatin
72 important microbial species
73 inhibition
74 interaction
75 isoform of HMG
76 isoforms
77 key substrate
78 levels
79 liquid chromatography mass spectrometry protocol
80 mass spectrometry protocol
81 medications
82 medium
83 mevalonate production
84 microbial cultures
85 microbial environment
86 microbial species
87 monocytogenes
88 nature
89 objective
90 pathogen Listeria monocytogenes
91 production
92 protocol
93 pure RSV
94 quantification
95 range
96 reductase
97 reduction
98 refined protocol
99 rosuvastatin
100 species
101 spectrometry approach
102 spectrometry protocol
103 statins
104 study
105 substrate
106 supernatant
107 system
108 ultra-performance liquid chromatography mass spectrometry protocol
109 work
110 schema:name Analysis of the Impact of Rosuvastatin on Bacterial Mevalonate Production Using a UPLC-Mass Spectrometry Approach
111 schema:pagination 1-8
112 schema:productId N4562f31f3b5f49ba8c607f9002532d8f
113 N678b9506a8274da78b3e504728591f16
114 Naf21ba4ec06548208937b1589db732b9
115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046418218
116 https://doi.org/10.1007/s00284-016-1014-z
117 schema:sdDatePublished 2021-11-01T18:27
118 schema:sdLicense https://scigraph.springernature.com/explorer/license/
119 schema:sdPublisher N0981ef61f4de4b73ac88f525b3771aa3
120 schema:url https://doi.org/10.1007/s00284-016-1014-z
121 sgo:license sg:explorer/license/
122 sgo:sdDataset articles
123 rdf:type schema:ScholarlyArticle
124 N0981ef61f4de4b73ac88f525b3771aa3 schema:name Springer Nature - SN SciGraph project
125 rdf:type schema:Organization
126 N1bd25247b2334ddb84ca12b43ad9783c rdf:first sg:person.0775107227.48
127 rdf:rest N592864bdcdb0474bb1ccf9276de55449
128 N2699fe496d46463eae37831d061399e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Mevalonic Acid
130 rdf:type schema:DefinedTerm
131 N2a7e89e439c74b6b919c8ea4959d7be9 rdf:first sg:person.0605260211.71
132 rdf:rest N336546b3ca9644b8a609d1dcc033db2e
133 N336546b3ca9644b8a609d1dcc033db2e rdf:first sg:person.014255126037.78
134 rdf:rest rdf:nil
135 N4562f31f3b5f49ba8c607f9002532d8f schema:name doi
136 schema:value 10.1007/s00284-016-1014-z
137 rdf:type schema:PropertyValue
138 N592864bdcdb0474bb1ccf9276de55449 rdf:first sg:person.01161643776.94
139 rdf:rest N5aca9019cee348438a917e4624f4c7ba
140 N5aca9019cee348438a917e4624f4c7ba rdf:first sg:person.01202224511.99
141 rdf:rest N2a7e89e439c74b6b919c8ea4959d7be9
142 N678b9506a8274da78b3e504728591f16 schema:name pubmed_id
143 schema:value 26960292
144 rdf:type schema:PropertyValue
145 N7e1c79a0e476459a80d3ae4b3f898992 schema:volumeNumber 73
146 rdf:type schema:PublicationVolume
147 N807d8bebc9bb4a0bbce98384de5ad32c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Rosuvastatin Calcium
149 rdf:type schema:DefinedTerm
150 N9e6a86bd249d4edea1401532168b771c schema:issueNumber 1
151 rdf:type schema:PublicationIssue
152 Naf21ba4ec06548208937b1589db732b9 schema:name dimensions_id
153 schema:value pub.1046418218
154 rdf:type schema:PropertyValue
155 Nbd6333b15c804487a3536916d47e28e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Listeria monocytogenes
157 rdf:type schema:DefinedTerm
158 Nd046ba8fceda45adafc6569d73feb93b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Staphylococcus aureus
160 rdf:type schema:DefinedTerm
161 Ne0786e3b63964c80aafe2564c12e05b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Mass Spectrometry
163 rdf:type schema:DefinedTerm
164 Ne96f4b702e3841dfb776c51296666acd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Chromatography, High Pressure Liquid
166 rdf:type schema:DefinedTerm
167 Neb86797453ae452cbd56b5071527d5e3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name Enterococcus faecium
169 rdf:type schema:DefinedTerm
170 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
171 schema:name Biological Sciences
172 rdf:type schema:DefinedTerm
173 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
174 schema:name Microbiology
175 rdf:type schema:DefinedTerm
176 sg:grant.3984108 http://pending.schema.org/fundedItem sg:pub.10.1007/s00284-016-1014-z
177 rdf:type schema:MonetaryGrant
178 sg:journal.1088675 schema:issn 0343-8651
179 1432-0991
180 schema:name Current Microbiology
181 schema:publisher Springer Nature
182 rdf:type schema:Periodical
183 sg:person.01161643776.94 schema:affiliation grid-institutes:grid.24349.38
184 schema:familyName Kinsella
185 schema:givenName M.
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01161643776.94
187 rdf:type schema:Person
188 sg:person.01202224511.99 schema:affiliation grid-institutes:grid.7872.a
189 schema:familyName Hill
190 schema:givenName C.
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01202224511.99
192 rdf:type schema:Person
193 sg:person.014255126037.78 schema:affiliation grid-institutes:grid.7872.a
194 schema:familyName Gahan
195 schema:givenName C. G. M.
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014255126037.78
197 rdf:type schema:Person
198 sg:person.0605260211.71 schema:affiliation grid-institutes:grid.7872.a
199 schema:familyName Joyce
200 schema:givenName S. A.
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605260211.71
202 rdf:type schema:Person
203 sg:person.0775107227.48 schema:affiliation grid-institutes:grid.7872.a
204 schema:familyName Nolan
205 schema:givenName J. A.
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0775107227.48
207 rdf:type schema:Person
208 sg:pub.10.1038/ja.2012.95 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008567184
209 https://doi.org/10.1038/ja.2012.95
210 rdf:type schema:CreativeWork
211 sg:pub.10.1038/nbt.1557 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022462669
212 https://doi.org/10.1038/nbt.1557
213 rdf:type schema:CreativeWork
214 sg:pub.10.1186/1476-0711-11-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040573060
215 https://doi.org/10.1186/1476-0711-11-13
216 rdf:type schema:CreativeWork
217 grid-institutes:grid.24349.38 schema:alternateName Department of Science, School of Science & Computing, Waterford Institute of Technology, Waterford, Ireland
218 schema:name Department of Science, School of Science & Computing, Waterford Institute of Technology, Waterford, Ireland
219 School of Food and Nutritional Sciences, University College Cork, Cork, Ireland
220 rdf:type schema:Organization
221 grid-institutes:grid.7872.a schema:alternateName School of Biochemistry & Cell Biology, University College Cork, Cork, Ireland
222 School of Microbiology, University College Cork, Cork, Ireland
223 School of Pharmacy, University College Cork, Cork, Ireland
224 schema:name APC Microbiome Institute, University College Cork, Cork, Ireland
225 School of Biochemistry & Cell Biology, University College Cork, Cork, Ireland
226 School of Microbiology, University College Cork, Cork, Ireland
227 School of Pharmacy, University College Cork, Cork, Ireland
228 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...