Quantitative Analysis of Porcine Endogenous Retroviruses in Different Organs of Transgenic Pigs Generated for Xenotransplantation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-06-02

AUTHORS

Urszula Mazurek, Magdalena C. Kimsa, Barbara Strzalka-Mrozik, Malgorzata W. Kimsa, Jolanta Adamska, Daniel Lipinski, Joanna Zeyland, Marlena Szalata, Ryszard Slomski, Jacek Jura, Zdzislaw Smorag, Roman Nowak, Joanna Gola

ABSTRACT

The pig appears to be the most promising animal donor of organs for use in human recipients. Among several types of pathogens found in pigs, one of the greatest problems is presented by porcine endogenous retroviruses (PERVs). Screening of the source pig herd for PERVs should include analysis of both PERV DNA and RNA. Therefore, the present study focuses on quantitative analysis of PERVs in different organs such as the skin, heart, muscle, and liver and blood of transgenic pigs generated for xenotransplantation. Transgenic pigs were developed to express the human α-galactosidase, the human α-1,2-fucosyltransferase gene, or both genetic modifications of the genome (Lipinski et al., Medycyna Wet 66:316–322, 2010; Lipinski et al., Ann Anim Sci 12:349–356, 2012; Wieczorek et al., Medycyna Wet 67:462–466, 2011). The copy numbers of PERV DNA and RNA were evaluated using real-time Q-PCR and QRT-PCR, respectively. Comparative analysis of all PERV subtypes revealed the following relationships: PERV A > PERV B > PERV C. PERV A and B were found in all samples, whereas PERV C was detected in 47 % of the tested animals. The lowest level of PERV DNA was shown in the muscles for PERV A and B and in blood samples for PERV C. The lowest level of PERV A RNA was found in the skin, whereas those of PERV B and C RNA were found in liver specimens. Quantitative analysis revealed differences in the copy number of PERV subtypes between various organs of transgenic pigs generated for xenotransplantation. Our data support the idea that careful pig selection for organ donation with low PERV copy number may limit the risk of retrovirus transmission to the human recipients. More... »

PAGES

505-514

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00284-013-0397-3

DOI

http://dx.doi.org/10.1007/s00284-013-0397-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024984459

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23728786


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39 PERV C. PERV A
40 PERV DNA
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42 PERV subtypes
43 RNA
44 analysis
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46 animals
47 blood
48 blood samples
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51 copy number
52 data
53 differences
54 different organs
55 donation
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57 endogenous retroviruses
58 fucosyltransferase gene
59 galactosidase
60 genes
61 genetic modification
62 genome
63 great problem
64 heart
65 herds
66 human recipients
67 idea
68 levels
69 liver
70 liver specimens
71 low PERV copy number
72 low levels
73 modification
74 muscle
75 number
76 organ donation
77 organs
78 pathogens
79 pig herds
80 pig selection
81 pigs
82 porcine endogenous retrovirus
83 present study
84 problem
85 promising animal donor
86 qRT-PCR
87 quantitative analysis
88 recipients
89 relationship
90 retrovirus transmission
91 retroviruses
92 risk
93 samples
94 selection
95 skin
96 source pig herd
97 specimens
98 study
99 subtypes
100 transgenic pigs
101 transmission
102 types
103 types of pathogens
104 use
105 xenotransplantation
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