Expression of dha Operon Required for 1,3-PD Formation in Escherichia coli and Saccharomyces cerevisiae View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-03

AUTHORS

Zheng Ma, Zhiming Rao, Liyu Xu, Xiangru Liao, Huiying Fang, Bin Zhuge, Jian Zhuge

ABSTRACT

The 1,3-propanediol (1,3-PD) synthesis operon (dha operon) was mainly composed of four genes: dhaB, dhaT, gdrA, and gdrB, which encoded glycerol dehydratase, 1,3-PD oxidoreductase and reactivating factor for glycerol dehydratase, respectively. In the present study, dha operon was cloned from 1,3-PD producing strain Klebsiella pneumoniae. Heterologous expression of cloned dha operon was carried out in Escherichia coli and Saccharomyces cerevisiae W303-1A, respectively. The results indicated that recombinant E. coli harboring the dha operon can produce 8-9 g/l 1,3-PD from glycerol while the 1,3-PD yield of recombinant strain W303-1A-dha could not be detected. In order to complete the 1,3-PD production from glucose, further, we also constructed the recombinant S. cerevisiae W303-1A-BT harboring plasmid pZ-BT. The 1,3-PD production and enzymatic activities of DhaB and DhaT were found in the engineered strain W303-1A-BT. Our results demonstrated that the recombinant S. cerevisiae strain W303-1A-BT that can produce 1,3-PD at low cost was constructed. This study might open a novel way to a safe and cost-efficient method for microbial production of 1,3-PD. More... »

PAGES

191-198

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00284-009-9528-2

DOI

http://dx.doi.org/10.1007/s00284-009-9528-2

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https://app.dimensions.ai/details/publication/pub.1041690763

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19924483


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