Identification and Functional Analysis of dTDP-Glucose-4,6-Dehydratase Gene and Its Linked Gene Cluster in an Aminoglycoside Antibiotics Producer of Streptomyces tenebrarius ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-08

AUTHORS

Yu Du, Tianbo Li, Yiguang G. Wang, Huanzhang Xia

ABSTRACT

Streptomyces tenebrarius H6 produces a variety of aminoglycoside antibiotics, such as apramycin, tobramycin, and kanamycin B. Primers were designed according to the highly conserved sequences of the dTDP-glucose-4,6-dehydratase genes, and a 0.6-kb PCR product was obtained from S. tenebrarius H6 genomic DNA. With the 0.6-kb PCR product as a probe, a BamHI 7.0-kb fragment was isolated. DNA sequence analysis of the 7.0-kb fragment revealed four ORFs and an incomplete ORF. In search of databases, the deduced product of one ORF (orfE) showed 62% identity to the dTDP-glucose-4,6-dehydratase, StrE of S. griseus. Three other ORFs (orfG1, orfG2, and orfGM) showed 55%, 62%, and 42% similarities, respectively, to glycosyltransferase from Clostridium acetobutylicum and mannosyltransferase from Xanthomonas axonopodis pv. citri str. 306 and glycosyltransferase from Pseudomonas putida KT2440. Upstream of the orfE was an incomplete ORF, and the deduced product showed 56% similarity to dTDP-4-dehydrorhamnose, StrL from S. griseus. The function of the orfE gene was studied by targeted gene disruption. The resulting mutant failed to produce tobramycin and kanamycin B, but still produced apramycin, suggesting that the orfE gene and linked gene cluster are essential for the biosynthesis of tobramycin and kanamycin B in S. tenebrarius H6. More... »

PAGES

99-107

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00284-004-4212-z

DOI

http://dx.doi.org/10.1007/s00284-004-4212-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004342155

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15297914


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosomes, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Clostridium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Deletion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Order", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glycosyltransferases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydro-Lyases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kanamycin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mannosyltransferases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Multigene Family", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis, Insertional", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nebramycin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Open Reading Frames", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pseudomonas putida", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rhamnose", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Streptomyces", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thymine Nucleotides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tobramycin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Xanthomonas", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Tsinghua University", 
          "id": "https://www.grid.ac/institutes/grid.12527.33", 
          "name": [
            "Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Tiantan Xili No1,, Beijing 100050, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Du", 
        "givenName": "Yu", 
        "id": "sg:person.0757353717.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0757353717.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tsinghua University", 
          "id": "https://www.grid.ac/institutes/grid.12527.33", 
          "name": [
            "Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Tiantan Xili No1,, Beijing 100050, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Tianbo", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tsinghua University", 
          "id": "https://www.grid.ac/institutes/grid.12527.33", 
          "name": [
            "Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Tiantan Xili No1,, Beijing 100050, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Yiguang G.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tsinghua University", 
          "id": "https://www.grid.ac/institutes/grid.12527.33", 
          "name": [
            "Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Tiantan Xili No1,, Beijing 100050, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xia", 
        "givenName": "Huanzhang", 
        "id": "sg:person.0701064623.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0701064623.69"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2004-08", 
    "datePublishedReg": "2004-08-01", 
    "description": "Streptomyces tenebrarius H6 produces a variety of aminoglycoside antibiotics, such as apramycin, tobramycin, and kanamycin B. Primers were designed according to the highly conserved sequences of the dTDP-glucose-4,6-dehydratase genes, and a 0.6-kb PCR product was obtained from S. tenebrarius H6 genomic DNA. With the 0.6-kb PCR product as a probe, a BamHI 7.0-kb fragment was isolated. DNA sequence analysis of the 7.0-kb fragment revealed four ORFs and an incomplete ORF. In search of databases, the deduced product of one ORF (orfE) showed 62% identity to the dTDP-glucose-4,6-dehydratase, StrE of S. griseus. Three other ORFs (orfG1, orfG2, and orfGM) showed 55%, 62%, and 42% similarities, respectively, to glycosyltransferase from Clostridium acetobutylicum and mannosyltransferase from Xanthomonas axonopodis pv. citri str. 306 and glycosyltransferase from Pseudomonas putida KT2440. Upstream of the orfE was an incomplete ORF, and the deduced product showed 56% similarity to dTDP-4-dehydrorhamnose, StrL from S. griseus. The function of the orfE gene was studied by targeted gene disruption. The resulting mutant failed to produce tobramycin and kanamycin B, but still produced apramycin, suggesting that the orfE gene and linked gene cluster are essential for the biosynthesis of tobramycin and kanamycin B in S. tenebrarius H6.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00284-004-4212-z", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1088675", 
        "issn": [
          "0343-8651", 
          "1432-0991"
        ], 
        "name": "Current Microbiology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "49"
      }
    ], 
    "name": "Identification and Functional Analysis of dTDP-Glucose-4,6-Dehydratase Gene and Its Linked Gene Cluster in an Aminoglycoside Antibiotics Producer of Streptomyces tenebrarius H6", 
    "pagination": "99-107", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "14b0864ad95a89d37af372e6af0c62ad98635d3f4929c82d4b32ec4943959b64"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "15297914"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "7808448"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00284-004-4212-z"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1004342155"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00284-004-4212-z", 
      "https://app.dimensions.ai/details/publication/pub.1004342155"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T14:09", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000510.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00284-004-4212-z"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00284-004-4212-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00284-004-4212-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00284-004-4212-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00284-004-4212-z'


 

This table displays all metadata directly associated to this object as RDF triples.

184 TRIPLES      20 PREDICATES      53 URIs      45 LITERALS      33 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00284-004-4212-z schema:about N005c612412d84d6ab25461d2e31fd020
2 N273799f320e84c3ea129cd5bd6280bd3
3 N273d563f57e543798815ba956e53de52
4 N29f00cf78e854e658538f6862c4368b8
5 N3b5276448a4448a1b931f9859f195b48
6 N4c2bab70fa594c05b0dbbd0cfc81520e
7 N61fd75db6da4498ca569d700a0794558
8 N6679e53117a74747a889b96a75b28351
9 N81beb10702e04d4289da695b92ccc93e
10 N820ea807c6b948eaaca364d3095aa6bc
11 N95e4ace25f404775b075fcaf47ebe0b8
12 Na2a531c5e0ab4d72ae40a7b3e3b3f758
13 Nabdfb89425be491fa2acfc85232e8067
14 Nb830e6366776485a9b006a9d58bc5120
15 Nc9eae0ec9a4d45c480c2e5d63fab0409
16 Ncbcffd5967fd4040ba74951714885d0f
17 Ncbfcb4f0b5094702a6920e839ff85ee1
18 Ne111f18e79de4d448b402fac191f0cc1
19 Ne372732b741d46f7b037fb0fcff4466e
20 Ne603b85bdd4842759c9706fb12d5a9c3
21 Ne8af9ccff6f34e18ae6d6254fadaf7c4
22 Ne9386231deca49a1b1071231fc7a9ff3
23 Nee770479c40646078461ae6a29d282b0
24 Nf80f43745fcd4d328d7f548f5cfd0fe0
25 anzsrc-for:06
26 anzsrc-for:0604
27 schema:author N029604b35b5a4b20a04d8ac6193490c4
28 schema:datePublished 2004-08
29 schema:datePublishedReg 2004-08-01
30 schema:description Streptomyces tenebrarius H6 produces a variety of aminoglycoside antibiotics, such as apramycin, tobramycin, and kanamycin B. Primers were designed according to the highly conserved sequences of the dTDP-glucose-4,6-dehydratase genes, and a 0.6-kb PCR product was obtained from S. tenebrarius H6 genomic DNA. With the 0.6-kb PCR product as a probe, a BamHI 7.0-kb fragment was isolated. DNA sequence analysis of the 7.0-kb fragment revealed four ORFs and an incomplete ORF. In search of databases, the deduced product of one ORF (orfE) showed 62% identity to the dTDP-glucose-4,6-dehydratase, StrE of S. griseus. Three other ORFs (orfG1, orfG2, and orfGM) showed 55%, 62%, and 42% similarities, respectively, to glycosyltransferase from Clostridium acetobutylicum and mannosyltransferase from Xanthomonas axonopodis pv. citri str. 306 and glycosyltransferase from Pseudomonas putida KT2440. Upstream of the orfE was an incomplete ORF, and the deduced product showed 56% similarity to dTDP-4-dehydrorhamnose, StrL from S. griseus. The function of the orfE gene was studied by targeted gene disruption. The resulting mutant failed to produce tobramycin and kanamycin B, but still produced apramycin, suggesting that the orfE gene and linked gene cluster are essential for the biosynthesis of tobramycin and kanamycin B in S. tenebrarius H6.
31 schema:genre research_article
32 schema:inLanguage en
33 schema:isAccessibleForFree false
34 schema:isPartOf Nb45e363a9367484c8d70fabaca2e8b7c
35 Ne30b8d904eb543e59ec1601faa74a266
36 sg:journal.1088675
37 schema:name Identification and Functional Analysis of dTDP-Glucose-4,6-Dehydratase Gene and Its Linked Gene Cluster in an Aminoglycoside Antibiotics Producer of Streptomyces tenebrarius H6
38 schema:pagination 99-107
39 schema:productId N93171e5dcad54622ab84259ef081d663
40 N974fd01c69e54563852c9e7dad251c6b
41 Ncadc9a153b8d4423a05cb8c64027f0e4
42 Ndd50718e0d0f42e0b45c61517c46411a
43 Necb3f22bd4284012bfedeb94543ac0af
44 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004342155
45 https://doi.org/10.1007/s00284-004-4212-z
46 schema:sdDatePublished 2019-04-10T14:09
47 schema:sdLicense https://scigraph.springernature.com/explorer/license/
48 schema:sdPublisher N8df34f7e25444b74ad1f196d939e2d86
49 schema:url http://link.springer.com/10.1007%2Fs00284-004-4212-z
50 sgo:license sg:explorer/license/
51 sgo:sdDataset articles
52 rdf:type schema:ScholarlyArticle
53 N005c612412d84d6ab25461d2e31fd020 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
54 schema:name Thymine Nucleotides
55 rdf:type schema:DefinedTerm
56 N029604b35b5a4b20a04d8ac6193490c4 rdf:first sg:person.0757353717.13
57 rdf:rest Na224b6f0fbab4358984d3e5e5582d217
58 N273799f320e84c3ea129cd5bd6280bd3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
59 schema:name Mannosyltransferases
60 rdf:type schema:DefinedTerm
61 N273d563f57e543798815ba956e53de52 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
62 schema:name Kanamycin
63 rdf:type schema:DefinedTerm
64 N29f00cf78e854e658538f6862c4368b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
65 schema:name Nebramycin
66 rdf:type schema:DefinedTerm
67 N3b5276448a4448a1b931f9859f195b48 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Glycosyltransferases
69 rdf:type schema:DefinedTerm
70 N4c2bab70fa594c05b0dbbd0cfc81520e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Genes, Bacterial
72 rdf:type schema:DefinedTerm
73 N5af01ab267fb40b2bcc319b7c2e68b46 rdf:first N9a8ed00644524f9789a4c5c94a42fec3
74 rdf:rest N849d63939363470a99ae0db9e46464b5
75 N61fd75db6da4498ca569d700a0794558 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Mutagenesis, Insertional
77 rdf:type schema:DefinedTerm
78 N6679e53117a74747a889b96a75b28351 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Molecular Sequence Data
80 rdf:type schema:DefinedTerm
81 N81beb10702e04d4289da695b92ccc93e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Hydro-Lyases
83 rdf:type schema:DefinedTerm
84 N820ea807c6b948eaaca364d3095aa6bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Open Reading Frames
86 rdf:type schema:DefinedTerm
87 N849d63939363470a99ae0db9e46464b5 rdf:first sg:person.0701064623.69
88 rdf:rest rdf:nil
89 N8df34f7e25444b74ad1f196d939e2d86 schema:name Springer Nature - SN SciGraph project
90 rdf:type schema:Organization
91 N919de407f9ce43c1b22677f834f8baf5 schema:affiliation https://www.grid.ac/institutes/grid.12527.33
92 schema:familyName Li
93 schema:givenName Tianbo
94 rdf:type schema:Person
95 N93171e5dcad54622ab84259ef081d663 schema:name pubmed_id
96 schema:value 15297914
97 rdf:type schema:PropertyValue
98 N95e4ace25f404775b075fcaf47ebe0b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name DNA, Bacterial
100 rdf:type schema:DefinedTerm
101 N974fd01c69e54563852c9e7dad251c6b schema:name doi
102 schema:value 10.1007/s00284-004-4212-z
103 rdf:type schema:PropertyValue
104 N9a8ed00644524f9789a4c5c94a42fec3 schema:affiliation https://www.grid.ac/institutes/grid.12527.33
105 schema:familyName Wang
106 schema:givenName Yiguang G.
107 rdf:type schema:Person
108 Na224b6f0fbab4358984d3e5e5582d217 rdf:first N919de407f9ce43c1b22677f834f8baf5
109 rdf:rest N5af01ab267fb40b2bcc319b7c2e68b46
110 Na2a531c5e0ab4d72ae40a7b3e3b3f758 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Tobramycin
112 rdf:type schema:DefinedTerm
113 Nabdfb89425be491fa2acfc85232e8067 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Sequence Homology
115 rdf:type schema:DefinedTerm
116 Nb45e363a9367484c8d70fabaca2e8b7c schema:issueNumber 2
117 rdf:type schema:PublicationIssue
118 Nb830e6366776485a9b006a9d58bc5120 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Gene Deletion
120 rdf:type schema:DefinedTerm
121 Nc9eae0ec9a4d45c480c2e5d63fab0409 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Xanthomonas
123 rdf:type schema:DefinedTerm
124 Ncadc9a153b8d4423a05cb8c64027f0e4 schema:name readcube_id
125 schema:value 14b0864ad95a89d37af372e6af0c62ad98635d3f4929c82d4b32ec4943959b64
126 rdf:type schema:PropertyValue
127 Ncbcffd5967fd4040ba74951714885d0f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Multigene Family
129 rdf:type schema:DefinedTerm
130 Ncbfcb4f0b5094702a6920e839ff85ee1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Rhamnose
132 rdf:type schema:DefinedTerm
133 Ndd50718e0d0f42e0b45c61517c46411a schema:name dimensions_id
134 schema:value pub.1004342155
135 rdf:type schema:PropertyValue
136 Ne111f18e79de4d448b402fac191f0cc1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Gene Order
138 rdf:type schema:DefinedTerm
139 Ne30b8d904eb543e59ec1601faa74a266 schema:volumeNumber 49
140 rdf:type schema:PublicationVolume
141 Ne372732b741d46f7b037fb0fcff4466e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Streptomyces
143 rdf:type schema:DefinedTerm
144 Ne603b85bdd4842759c9706fb12d5a9c3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Clostridium
146 rdf:type schema:DefinedTerm
147 Ne8af9ccff6f34e18ae6d6254fadaf7c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Sequence Analysis, DNA
149 rdf:type schema:DefinedTerm
150 Ne9386231deca49a1b1071231fc7a9ff3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Amino Acid Sequence
152 rdf:type schema:DefinedTerm
153 Necb3f22bd4284012bfedeb94543ac0af schema:name nlm_unique_id
154 schema:value 7808448
155 rdf:type schema:PropertyValue
156 Nee770479c40646078461ae6a29d282b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Chromosomes, Bacterial
158 rdf:type schema:DefinedTerm
159 Nf80f43745fcd4d328d7f548f5cfd0fe0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Pseudomonas putida
161 rdf:type schema:DefinedTerm
162 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
163 schema:name Biological Sciences
164 rdf:type schema:DefinedTerm
165 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
166 schema:name Genetics
167 rdf:type schema:DefinedTerm
168 sg:journal.1088675 schema:issn 0343-8651
169 1432-0991
170 schema:name Current Microbiology
171 rdf:type schema:Periodical
172 sg:person.0701064623.69 schema:affiliation https://www.grid.ac/institutes/grid.12527.33
173 schema:familyName Xia
174 schema:givenName Huanzhang
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0701064623.69
176 rdf:type schema:Person
177 sg:person.0757353717.13 schema:affiliation https://www.grid.ac/institutes/grid.12527.33
178 schema:familyName Du
179 schema:givenName Yu
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0757353717.13
181 rdf:type schema:Person
182 https://www.grid.ac/institutes/grid.12527.33 schema:alternateName Tsinghua University
183 schema:name Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Tiantan Xili No1,, Beijing 100050, China
184 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...