Delivery of a Genetically Marked Alcaligenes sp. to the Glassy-Winged Sharpshooter for Use in a Paratransgenic Control Strategy View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-05

AUTHORS

Blake Bextine, Carol Lauzon, Sarah Potter, David Lampe, Thomas A. Miller

ABSTRACT

An artificial feeding system was designed for the glassy-winged sharpshooter (GWSS), Homalodisca coagulata Say (Hemiptera: Cicadellidae). The system, unlike previous systems, provided enough nutrients to GWSS to survive for 48 h. A system like this is a prerequisite to examining the potential use of paratransgenesis to interrupt transmission of Xylella fastidiosa, the bacterial pathogen causing Pierce's disease of grape, by insect vectors. We developed a system for short-term feeding of GWSS that allows for the introduction of bacteria in liquid medium, and we have demonstrated the ability of Alcaligenes xylosoxidans denitrificans, expressing a red fluorescent protein (dsRed), to colonize the cibarial region of the GWSS foregut for up to 5 weeks post-exposure. Alcaligenes xylosoxidans denitrificans thus occupies the same region in the foregut as the pathogen, Xylella fastidiosa. More... »

PAGES

327-331

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00284-003-4178-2

DOI

http://dx.doi.org/10.1007/s00284-003-4178-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034305668

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15060727


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