Transformed Bacterial Symbionts Re-Introduced to and Detected in Host Gut View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-07

AUTHORS

John J. Peloquin, Carol R. Lauzon, Sarah Potter, Thomas A. Miller

ABSTRACT

Strains of Enterobacter agglomerans and Klebsiella pneumoniae isolated from Rhagoletis completa Cresson were engineered to express transgenic fluorescent proteins (ECFP, DsRed). These bacteria were introduced into flies by feeding the flies a sucrose solution in which the bacteria were suspended. The transgenic and heterologous marker protein was expressed and visible in the bacteria after they were ingested by WHF and while they were in the fly gut. We describe the plasmids used to transform these bacteria and demonstrate expression of heterologous proteins from the transforming plasmids and discuss the implications for future pest control strategies. More... »

PAGES

41-45

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00284-001-0045-1

DOI

http://dx.doi.org/10.1007/s00284-001-0045-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052763217

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12029526


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