Pretreatment with anti-oxidants sensitizes oxidatively stressed human cancer cells to growth inhibitory effect of suberoylanilide hydroxamic acid (SAHA) View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-03

AUTHORS

Hirak S. Basu, Amy Mahlum, Farideh Mehraein-Ghomi, Stacy J. Kegel, Song Guo, Noel R. Peters, George Wilding

ABSTRACT

PURPOSE: Most prostate, colon and breast cancer cells are resistant to growth inhibitory effects of suberoylanilide hydroxamic acid (SAHA). We have examined whether the high oxidative stress in these cells causes a loss of SAHA activity and if so, whether pretreatment with an anti-oxidant can sensitize these cells to SAHA. METHODS: A DNA-Hoechst dye fluorescence measured cell growth and dichlorfluorescein-diacetate (DCF-DA) dye fluorescence measured reactive oxygen species (ROS). Growth inhibitory and ROS-generating activities of SAHA in androgen-treated or untreated LNCaP cells and PC-3 prostate cancer cells, HT-29 and HCT-115 colon cancer cells, MDA-MB231 breast cancer cells and A549 and NCI-H460 lung cancer cells with or without pretreatment with an anti-oxidant Vitamin E was determined. SAHA activity against LNCaP cells treated with another anti-oxidant N-acetyl cysteine (NAC) was also determined. Liquid chromatography-mass spectrometry (LC-MS) was used to determine intracellular SAHA level. RESULTS: SAHA treatment markedly inhibits LNCaP cell growth, when the cells are at a low ROS level. SAHA is, however, inactive against the same cell line, when the cells are at a high ROS level. A significant decrease in SAHA level was observed in LNCaP cells with high ROS after 24- and 72-h treatment when compared to cells with low ROS. Vitamin E pretreatment that reduces cellular ROS, synergistically sensitizes oxidatively stressed LNCaP, PC-3, HT-29, HCT-115 and MDA-MB231 cells, but not the A-549 and NCI-H460 cells with low ROS to SAHA. NAC treatment also sensitized androgen-treated LNCaP cells to the growth inhibitory effects of SAHA. CONCLUSION: Response to SAHA could be improved by combining anti-oxidants such as Vitamin E with SAHA for the treatment of oxidatively stressed human malignancies that are otherwise resistant to SAHA. More... »

PAGES

705-715

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00280-010-1364-3

DOI

http://dx.doi.org/10.1007/s00280-010-1364-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044422223

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20512578


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antioxidants", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Breast Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatography, Liquid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Colonic Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Resistance, Neoplasm", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Synergism", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Histone Deacetylase Inhibitors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydroxamic Acids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mass Spectrometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oxidative Stress", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Prostatic Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reactive Oxygen Species", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Vitamin E", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Wisconsin Institutes for Medical Research, University of Wisconsin Carbone Comprehensive Cancer Center, 1111 Highland Avenue, 53705, Madison, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Basu", 
        "givenName": "Hirak S.", 
        "id": "sg:person.0614762647.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614762647.80"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Wisconsin Institutes for Medical Research, University of Wisconsin Carbone Comprehensive Cancer Center, 1111 Highland Avenue, 53705, Madison, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mahlum", 
        "givenName": "Amy", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Wisconsin Institutes for Medical Research, University of Wisconsin Carbone Comprehensive Cancer Center, 1111 Highland Avenue, 53705, Madison, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mehraein-Ghomi", 
        "givenName": "Farideh", 
        "id": "sg:person.01156025147.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156025147.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Wisconsin Institutes for Medical Research, University of Wisconsin Carbone Comprehensive Cancer Center, 1111 Highland Avenue, 53705, Madison, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kegel", 
        "givenName": "Stacy J.", 
        "id": "sg:person.0614312665.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614312665.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Wisconsin Institutes for Medical Research, University of Wisconsin Carbone Comprehensive Cancer Center, 1111 Highland Avenue, 53705, Madison, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Guo", 
        "givenName": "Song", 
        "id": "sg:person.0652040507.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652040507.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Wisconsin Institutes for Medical Research, University of Wisconsin Carbone Comprehensive Cancer Center, 1111 Highland Avenue, 53705, Madison, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Peters", 
        "givenName": "Noel R.", 
        "id": "sg:person.0603725307.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603725307.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Wisconsin Institutes for Medical Research, University of Wisconsin Carbone Comprehensive Cancer Center, 1111 Highland Avenue, 53705, Madison, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wilding", 
        "givenName": "George", 
        "id": "sg:person.016113722177.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016113722177.62"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1182/blood-2006-06-025999", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009387348"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jchromb.2006.04.044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011794121"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/mol.107.041293", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015320059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-291x(92)90471-v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015933583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1517/14728222.9.4.809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020021164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1517/14728222.9.4.809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020021164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/1078-0432.ccr-08-0122", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021006436"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1210610", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023418889", 
          "https://doi.org/10.1038/sj.onc.1210610"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/bcmd.2000.0376", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024627256"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0065-2571(84)90007-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026371633"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0065-2571(84)90007-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026371633"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jnci/89.1.40", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026838470"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/1534735404270578", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036129467"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/1534735404270578", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036129467"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10637-008-9131-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038891516", 
          "https://doi.org/10.1007/s10637-008-9131-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10637-008-9131-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038891516", 
          "https://doi.org/10.1007/s10637-008-9131-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.2005.14.167", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040412110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0408732102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046396110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/ppo.0b013e31803c72ba", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046575221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/ppo.0b013e31803c72ba", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046575221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1183/09031936.06.00053805", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050224488"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-08-2472", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051000980"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.freeradbiomed.2009.05.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052105117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.canlet.2009.02.042", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053415993"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/pr050266a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056290809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081983028", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082322946", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-03", 
    "datePublishedReg": "2011-03-01", 
    "description": "PURPOSE: Most prostate, colon and breast cancer cells are resistant to growth inhibitory effects of suberoylanilide hydroxamic acid (SAHA). We have examined whether the high oxidative stress in these cells causes a loss of SAHA activity and if so, whether pretreatment with an anti-oxidant can sensitize these cells to SAHA.\nMETHODS: A DNA-Hoechst dye fluorescence measured cell growth and dichlorfluorescein-diacetate (DCF-DA) dye fluorescence measured reactive oxygen species (ROS). Growth inhibitory and ROS-generating activities of SAHA in androgen-treated or untreated LNCaP cells and PC-3 prostate cancer cells, HT-29 and HCT-115 colon cancer cells, MDA-MB231 breast cancer cells and A549 and NCI-H460 lung cancer cells with or without pretreatment with an anti-oxidant Vitamin E was determined. SAHA activity against LNCaP cells treated with another anti-oxidant N-acetyl cysteine (NAC) was also determined. Liquid chromatography-mass spectrometry (LC-MS) was used to determine intracellular SAHA level.\nRESULTS: SAHA treatment markedly inhibits LNCaP cell growth, when the cells are at a low ROS level. SAHA is, however, inactive against the same cell line, when the cells are at a high ROS level. A significant decrease in SAHA level was observed in LNCaP cells with high ROS after 24- and 72-h treatment when compared to cells with low ROS. Vitamin E pretreatment that reduces cellular ROS, synergistically sensitizes oxidatively stressed LNCaP, PC-3, HT-29, HCT-115 and MDA-MB231 cells, but not the A-549 and NCI-H460 cells with low ROS to SAHA. NAC treatment also sensitized androgen-treated LNCaP cells to the growth inhibitory effects of SAHA.\nCONCLUSION: Response to SAHA could be improved by combining anti-oxidants such as Vitamin E with SAHA for the treatment of oxidatively stressed human malignancies that are otherwise resistant to SAHA.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00280-010-1364-3", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2438809", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1088364", 
        "issn": [
          "0344-5704", 
          "1432-0843"
        ], 
        "name": "Cancer Chemotherapy and Pharmacology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "67"
      }
    ], 
    "name": "Pretreatment with anti-oxidants sensitizes oxidatively stressed human cancer cells to growth inhibitory effect of suberoylanilide hydroxamic acid (SAHA)", 
    "pagination": "705-715", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d399760ce46374e73e77665f65967cf7964e86eeb7c404a4a17d5042542af85d"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "20512578"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "7806519"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00280-010-1364-3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1044422223"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00280-010-1364-3", 
      "https://app.dimensions.ai/details/publication/pub.1044422223"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T23:25", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000515.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00280-010-1364-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00280-010-1364-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00280-010-1364-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00280-010-1364-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00280-010-1364-3'


 

This table displays all metadata directly associated to this object as RDF triples.

257 TRIPLES      21 PREDICATES      68 URIs      38 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00280-010-1364-3 schema:about N281ead3bfdbf49c780fa27be23cdb507
2 N37bda19bdcce4f44b4f639fbc2add2b6
3 N3ee72d7115744156a1c1a9695cb03f6d
4 N3eeca09939954aa99a9b91c0d8252c38
5 N41b0639b0a604a9c81b446590b31ab94
6 N42aa7c139fd94aa4a63313c897afae64
7 N46daab99b31148e78f385bd6cc40864d
8 N47b2dc6c8b214a9c98f195cddfa4ac8c
9 N5c24d3368f734febb8bfc30668bcf1f7
10 N637a6bc386894f25a4da9c3b2b5a644b
11 N7840a8f6c8554f6a9c5c4f3d27bced87
12 N961710a516c24d48b8609e13770ad9da
13 Nb9d545176bfc4ce588a97db1b45c48a3
14 Nbd2d5bf8cb254722848b36bdf25fed5d
15 Nebb4f6c2d6bc4f209f51ebac77e8e679
16 Nf41a5d1a8ed94f64bc3c619ff57cbeb0
17 Nf43de3e5de0548b4ad24e75f52735008
18 anzsrc-for:11
19 anzsrc-for:1112
20 schema:author Nf14dead120994bfb991e80be1098a358
21 schema:citation sg:pub.10.1007/s10637-008-9131-6
22 sg:pub.10.1038/sj.onc.1210610
23 https://app.dimensions.ai/details/publication/pub.1081983028
24 https://app.dimensions.ai/details/publication/pub.1082322946
25 https://doi.org/10.1006/bcmd.2000.0376
26 https://doi.org/10.1016/0006-291x(92)90471-v
27 https://doi.org/10.1016/0065-2571(84)90007-4
28 https://doi.org/10.1016/j.canlet.2009.02.042
29 https://doi.org/10.1016/j.freeradbiomed.2009.05.011
30 https://doi.org/10.1016/j.jchromb.2006.04.044
31 https://doi.org/10.1021/pr050266a
32 https://doi.org/10.1073/pnas.0408732102
33 https://doi.org/10.1093/jnci/89.1.40
34 https://doi.org/10.1097/ppo.0b013e31803c72ba
35 https://doi.org/10.1124/mol.107.041293
36 https://doi.org/10.1158/0008-5472.can-08-2472
37 https://doi.org/10.1158/1078-0432.ccr-08-0122
38 https://doi.org/10.1177/1534735404270578
39 https://doi.org/10.1182/blood-2006-06-025999
40 https://doi.org/10.1183/09031936.06.00053805
41 https://doi.org/10.1200/jco.2005.14.167
42 https://doi.org/10.1517/14728222.9.4.809
43 schema:datePublished 2011-03
44 schema:datePublishedReg 2011-03-01
45 schema:description PURPOSE: Most prostate, colon and breast cancer cells are resistant to growth inhibitory effects of suberoylanilide hydroxamic acid (SAHA). We have examined whether the high oxidative stress in these cells causes a loss of SAHA activity and if so, whether pretreatment with an anti-oxidant can sensitize these cells to SAHA. METHODS: A DNA-Hoechst dye fluorescence measured cell growth and dichlorfluorescein-diacetate (DCF-DA) dye fluorescence measured reactive oxygen species (ROS). Growth inhibitory and ROS-generating activities of SAHA in androgen-treated or untreated LNCaP cells and PC-3 prostate cancer cells, HT-29 and HCT-115 colon cancer cells, MDA-MB231 breast cancer cells and A549 and NCI-H460 lung cancer cells with or without pretreatment with an anti-oxidant Vitamin E was determined. SAHA activity against LNCaP cells treated with another anti-oxidant N-acetyl cysteine (NAC) was also determined. Liquid chromatography-mass spectrometry (LC-MS) was used to determine intracellular SAHA level. RESULTS: SAHA treatment markedly inhibits LNCaP cell growth, when the cells are at a low ROS level. SAHA is, however, inactive against the same cell line, when the cells are at a high ROS level. A significant decrease in SAHA level was observed in LNCaP cells with high ROS after 24- and 72-h treatment when compared to cells with low ROS. Vitamin E pretreatment that reduces cellular ROS, synergistically sensitizes oxidatively stressed LNCaP, PC-3, HT-29, HCT-115 and MDA-MB231 cells, but not the A-549 and NCI-H460 cells with low ROS to SAHA. NAC treatment also sensitized androgen-treated LNCaP cells to the growth inhibitory effects of SAHA. CONCLUSION: Response to SAHA could be improved by combining anti-oxidants such as Vitamin E with SAHA for the treatment of oxidatively stressed human malignancies that are otherwise resistant to SAHA.
46 schema:genre research_article
47 schema:inLanguage en
48 schema:isAccessibleForFree true
49 schema:isPartOf N45398f0b94614d63bde40fe99ce92b83
50 Naddd675414fc4a3fa0cfdb1e2399aa9f
51 sg:journal.1088364
52 schema:name Pretreatment with anti-oxidants sensitizes oxidatively stressed human cancer cells to growth inhibitory effect of suberoylanilide hydroxamic acid (SAHA)
53 schema:pagination 705-715
54 schema:productId N4b5cc66b66bb4b0a9e0a2d8863a3988b
55 N5313e052865744be87693f99d03f1d77
56 N65df0ee346df4c60ab8996c66b4165bb
57 N6b646cdaf2a7415590b5776f7d260bce
58 Naa6e206edfd140afa5abbf4b426d02e8
59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044422223
60 https://doi.org/10.1007/s00280-010-1364-3
61 schema:sdDatePublished 2019-04-10T23:25
62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
63 schema:sdPublisher N20ae4dab512b4be2814c61c6cfd8c854
64 schema:url http://link.springer.com/10.1007%2Fs00280-010-1364-3
65 sgo:license sg:explorer/license/
66 sgo:sdDataset articles
67 rdf:type schema:ScholarlyArticle
68 N072b4bf160c34df5be145259f8ebf4c8 schema:name Wisconsin Institutes for Medical Research, University of Wisconsin Carbone Comprehensive Cancer Center, 1111 Highland Avenue, 53705, Madison, WI, USA
69 rdf:type schema:Organization
70 N0fe902b8de7c4eb5b791cfbf0694d960 schema:name Wisconsin Institutes for Medical Research, University of Wisconsin Carbone Comprehensive Cancer Center, 1111 Highland Avenue, 53705, Madison, WI, USA
71 rdf:type schema:Organization
72 N18ed879377cc45419ad492bdbd138b0d schema:affiliation Nf19f89865b8e4d3ea69d0694a977eaa1
73 schema:familyName Mahlum
74 schema:givenName Amy
75 rdf:type schema:Person
76 N20ae4dab512b4be2814c61c6cfd8c854 schema:name Springer Nature - SN SciGraph project
77 rdf:type schema:Organization
78 N281ead3bfdbf49c780fa27be23cdb507 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Female
80 rdf:type schema:DefinedTerm
81 N309b2d00433a4b2295fca18f22bd508f rdf:first sg:person.0603725307.49
82 rdf:rest N3e042af1ef7d44ac8dbdcfe70a4da522
83 N36c73ca993c64889b3f46012846af34d rdf:first sg:person.0614312665.04
84 rdf:rest N6e6de942da5a410bb1833e6ed5603067
85 N37bda19bdcce4f44b4f639fbc2add2b6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Hydroxamic Acids
87 rdf:type schema:DefinedTerm
88 N3bbf68b8d5da4d85a1b8138f867072f3 schema:name Wisconsin Institutes for Medical Research, University of Wisconsin Carbone Comprehensive Cancer Center, 1111 Highland Avenue, 53705, Madison, WI, USA
89 rdf:type schema:Organization
90 N3e042af1ef7d44ac8dbdcfe70a4da522 rdf:first sg:person.016113722177.62
91 rdf:rest rdf:nil
92 N3ee72d7115744156a1c1a9695cb03f6d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Humans
94 rdf:type schema:DefinedTerm
95 N3eeca09939954aa99a9b91c0d8252c38 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Chromatography, Liquid
97 rdf:type schema:DefinedTerm
98 N41b0639b0a604a9c81b446590b31ab94 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Male
100 rdf:type schema:DefinedTerm
101 N42aa7c139fd94aa4a63313c897afae64 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Drug Resistance, Neoplasm
103 rdf:type schema:DefinedTerm
104 N42e622f665f245b186f57c9a7ed063ca schema:name Wisconsin Institutes for Medical Research, University of Wisconsin Carbone Comprehensive Cancer Center, 1111 Highland Avenue, 53705, Madison, WI, USA
105 rdf:type schema:Organization
106 N45398f0b94614d63bde40fe99ce92b83 schema:issueNumber 3
107 rdf:type schema:PublicationIssue
108 N46daab99b31148e78f385bd6cc40864d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Oxidative Stress
110 rdf:type schema:DefinedTerm
111 N47b2dc6c8b214a9c98f195cddfa4ac8c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Cell Line, Tumor
113 rdf:type schema:DefinedTerm
114 N4b5cc66b66bb4b0a9e0a2d8863a3988b schema:name nlm_unique_id
115 schema:value 7806519
116 rdf:type schema:PropertyValue
117 N5313e052865744be87693f99d03f1d77 schema:name pubmed_id
118 schema:value 20512578
119 rdf:type schema:PropertyValue
120 N5c24d3368f734febb8bfc30668bcf1f7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Reactive Oxygen Species
122 rdf:type schema:DefinedTerm
123 N637a6bc386894f25a4da9c3b2b5a644b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Vitamin E
125 rdf:type schema:DefinedTerm
126 N65df0ee346df4c60ab8996c66b4165bb schema:name dimensions_id
127 schema:value pub.1044422223
128 rdf:type schema:PropertyValue
129 N6b646cdaf2a7415590b5776f7d260bce schema:name readcube_id
130 schema:value d399760ce46374e73e77665f65967cf7964e86eeb7c404a4a17d5042542af85d
131 rdf:type schema:PropertyValue
132 N6e6de942da5a410bb1833e6ed5603067 rdf:first sg:person.0652040507.29
133 rdf:rest N309b2d00433a4b2295fca18f22bd508f
134 N7840a8f6c8554f6a9c5c4f3d27bced87 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Drug Synergism
136 rdf:type schema:DefinedTerm
137 N961710a516c24d48b8609e13770ad9da schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Mass Spectrometry
139 rdf:type schema:DefinedTerm
140 Na813d4c3431442ff8d396a47f4953ca7 rdf:first N18ed879377cc45419ad492bdbd138b0d
141 rdf:rest Nd76205bd01c746f0b8f877831d63ed88
142 Naa6e206edfd140afa5abbf4b426d02e8 schema:name doi
143 schema:value 10.1007/s00280-010-1364-3
144 rdf:type schema:PropertyValue
145 Naddd675414fc4a3fa0cfdb1e2399aa9f schema:volumeNumber 67
146 rdf:type schema:PublicationVolume
147 Nb9d545176bfc4ce588a97db1b45c48a3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Colonic Neoplasms
149 rdf:type schema:DefinedTerm
150 Nbd2d5bf8cb254722848b36bdf25fed5d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Histone Deacetylase Inhibitors
152 rdf:type schema:DefinedTerm
153 Nd565803c75034a7eae5a89a4fa16bca4 schema:name Wisconsin Institutes for Medical Research, University of Wisconsin Carbone Comprehensive Cancer Center, 1111 Highland Avenue, 53705, Madison, WI, USA
154 rdf:type schema:Organization
155 Nd76205bd01c746f0b8f877831d63ed88 rdf:first sg:person.01156025147.58
156 rdf:rest N36c73ca993c64889b3f46012846af34d
157 Nea1e2026f7ab4db08c2785a36468c26f schema:name Wisconsin Institutes for Medical Research, University of Wisconsin Carbone Comprehensive Cancer Center, 1111 Highland Avenue, 53705, Madison, WI, USA
158 rdf:type schema:Organization
159 Nebb4f6c2d6bc4f209f51ebac77e8e679 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Breast Neoplasms
161 rdf:type schema:DefinedTerm
162 Nf14dead120994bfb991e80be1098a358 rdf:first sg:person.0614762647.80
163 rdf:rest Na813d4c3431442ff8d396a47f4953ca7
164 Nf19f89865b8e4d3ea69d0694a977eaa1 schema:name Wisconsin Institutes for Medical Research, University of Wisconsin Carbone Comprehensive Cancer Center, 1111 Highland Avenue, 53705, Madison, WI, USA
165 rdf:type schema:Organization
166 Nf41a5d1a8ed94f64bc3c619ff57cbeb0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Antioxidants
168 rdf:type schema:DefinedTerm
169 Nf43de3e5de0548b4ad24e75f52735008 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Prostatic Neoplasms
171 rdf:type schema:DefinedTerm
172 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
173 schema:name Medical and Health Sciences
174 rdf:type schema:DefinedTerm
175 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
176 schema:name Oncology and Carcinogenesis
177 rdf:type schema:DefinedTerm
178 sg:grant.2438809 http://pending.schema.org/fundedItem sg:pub.10.1007/s00280-010-1364-3
179 rdf:type schema:MonetaryGrant
180 sg:journal.1088364 schema:issn 0344-5704
181 1432-0843
182 schema:name Cancer Chemotherapy and Pharmacology
183 rdf:type schema:Periodical
184 sg:person.01156025147.58 schema:affiliation N072b4bf160c34df5be145259f8ebf4c8
185 schema:familyName Mehraein-Ghomi
186 schema:givenName Farideh
187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156025147.58
188 rdf:type schema:Person
189 sg:person.016113722177.62 schema:affiliation N0fe902b8de7c4eb5b791cfbf0694d960
190 schema:familyName Wilding
191 schema:givenName George
192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016113722177.62
193 rdf:type schema:Person
194 sg:person.0603725307.49 schema:affiliation Nd565803c75034a7eae5a89a4fa16bca4
195 schema:familyName Peters
196 schema:givenName Noel R.
197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603725307.49
198 rdf:type schema:Person
199 sg:person.0614312665.04 schema:affiliation Nea1e2026f7ab4db08c2785a36468c26f
200 schema:familyName Kegel
201 schema:givenName Stacy J.
202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614312665.04
203 rdf:type schema:Person
204 sg:person.0614762647.80 schema:affiliation N3bbf68b8d5da4d85a1b8138f867072f3
205 schema:familyName Basu
206 schema:givenName Hirak S.
207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614762647.80
208 rdf:type schema:Person
209 sg:person.0652040507.29 schema:affiliation N42e622f665f245b186f57c9a7ed063ca
210 schema:familyName Guo
211 schema:givenName Song
212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652040507.29
213 rdf:type schema:Person
214 sg:pub.10.1007/s10637-008-9131-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038891516
215 https://doi.org/10.1007/s10637-008-9131-6
216 rdf:type schema:CreativeWork
217 sg:pub.10.1038/sj.onc.1210610 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023418889
218 https://doi.org/10.1038/sj.onc.1210610
219 rdf:type schema:CreativeWork
220 https://app.dimensions.ai/details/publication/pub.1081983028 schema:CreativeWork
221 https://app.dimensions.ai/details/publication/pub.1082322946 schema:CreativeWork
222 https://doi.org/10.1006/bcmd.2000.0376 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024627256
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/0006-291x(92)90471-v schema:sameAs https://app.dimensions.ai/details/publication/pub.1015933583
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/0065-2571(84)90007-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026371633
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/j.canlet.2009.02.042 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053415993
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/j.freeradbiomed.2009.05.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052105117
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/j.jchromb.2006.04.044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011794121
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1021/pr050266a schema:sameAs https://app.dimensions.ai/details/publication/pub.1056290809
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1073/pnas.0408732102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046396110
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1093/jnci/89.1.40 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026838470
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1097/ppo.0b013e31803c72ba schema:sameAs https://app.dimensions.ai/details/publication/pub.1046575221
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1124/mol.107.041293 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015320059
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1158/0008-5472.can-08-2472 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051000980
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1158/1078-0432.ccr-08-0122 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021006436
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1177/1534735404270578 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036129467
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1182/blood-2006-06-025999 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009387348
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1183/09031936.06.00053805 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050224488
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1200/jco.2005.14.167 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040412110
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1517/14728222.9.4.809 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020021164
257 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...