Stimulation of intestinal epithelial restitution by prostaglandin E1 analogue View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-02-26

AUTHORS

Kohji Hirata, Toshiharu Horie

ABSTRACT

Background5-Fluorouracil (5-FU) causes intestinal mucosal damage and malabsorption. We have recently reported that coadministration of 17S,20-dimethyl-trans-⊿2-prostaglandin E1 (OP-1206), a stable synthetic analogue of prostaglandin E1, with 5-FU to rats protects the small intestine from 5-FU-induced damage. Enterocyte proliferation would contribute to the restitution of the wounded intestinal mucosa. Thus, we investigated the effect of OP-1206 on the proliferation of rat jejunal crypt cells (IEC-6 cells) treated with 5-FU.MethodsProliferation of IEC-6 cells was evaluated in terms of [3H]-thymidine incorporation and using the 3-(4,5-dimethyl-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Mucosal healing was assessed by measuring the speed of resealing across the denuded area of an IEC-6 cell monolayer.ResultsOP-1206 stimulated [3H]-thymidine incorporation into subconfluent IEC-6 cells pretreated with 5-FU and increased the number of IEC-6 cells. AH23848B, an EP4 prostaglandin receptor antagonist, blocked the OP-1206-stimulated [3H]-thymidine incorporation into IEC-6 cells. The speed of resealing across the denuded area of a wounded IEC-6 cell monolayer was found to increase following treatment with OP-1206.ConclusionsOP-1206 stimulated the proliferation of IEC-6 cells treated with 5-FU, indicating a possible mechanism for the protective effect of OP-1206 against 5-FU-induced damage to the small intestine. OP-1206 was shown to be active in intestinal mucosal healing. More... »

PAGES

216-220

References to SciGraph publications

  • 1996-07. Enhanced absorption of macromolecules in DIGESTIVE DISEASES AND SCIENCES
  • Identifiers

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    http://scigraph.springernature.com/pub.10.1007/s00280-003-0576-1

    DOI

    http://dx.doi.org/10.1007/s00280-003-0576-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1075257696

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12655439


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