Comparison of Cell Number, Viability, Phenotypic Profile, Clonogenic, and Proliferative Potential of Adipose-Derived Stem Cell Populations Between Centrifuged and Noncentrifuged ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-07-23

AUTHORS

Adalberto Ibatici, Fabio Caviggioli, Vinci Valeriano, Nadia Quirici, Nadia Sessarego, Andrea Lisa, Francesco Klinger, Davide Forcellini, Luca Maione, Marco Klinger

ABSTRACT

BackgroundAutologous fat grafting is a widely adopted surgical technique in both the reconstructive and aesthetic fields. This study aimed to compare centrifuged lipoaspirates harvested and refined by the Coleman technique with noncentrifuged lipoaspirates in terms of cell number and viability, phenotypic profile, and clonogenic and proliferative potential of adipose-derived stem cell (ADSC) populations.MethodsFor each patient, both a centrifuged sample using the Coleman’s technique and a noncentrifuged sample of adipose tissue were collected. Adipose-derived stem cells from both the centrifuged fraction (CF) and the noncentrifuged fraction (NCF) were isolated. The recovered ADSCs were used to set up flow cytometry analysis, colony-forming units–fibroblast (CFU-F) assays, and ADSC cultures.ResultsThe number of recovered cells was variable among the different donors but significantly higher in the CF donors. Cell viability, determined by the Trypan Blue dye assay, always exceeded 95 %, in both the CF and NCF fractions. Analysis of the putative ADSC subpopulations showed a significant enrichment of the mesenchymal and endothelial progenitors in CF compared with NCF. No differences in the clonogenic efficiency of the ADSC samples were observed when the same number of cells were plated from each fraction. On the contrary, when equal fat volumes were compared, the colony-forming ability of CF was always significantly higher than that of its NCF counterparts.ConclusionsThis is the first study to comprehensively characterize the impact of Coleman’s technique on the quality of lipoaspirates, showing that centrifugation is safe and feasible and does not impair cell viability, can augment the content in ADSC and the frequency of CFU-F, and reduces the number of proinflammatory blood cells.No Level AssignedThis journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors http://www.springer.com/00266. More... »

PAGES

985-993

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00266-014-0372-9

DOI

http://dx.doi.org/10.1007/s00266-014-0372-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1053093214

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25053112


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36 Evidence-Based Medicine rankings
37 Level AssignedThis journal
38 Medicine ratings
39 MethodsFor
40 NCF
41 Table of Contents
42 ability
43 adipose
44 adipose tissue
45 adipose-derived stem cells
46 aesthetic field
47 analysis
48 animal studies
49 article
50 assays
51 authors
52 basic science
53 blood cells
54 book reviews
55 cadaver study
56 cell number
57 cell populations
58 cell viability
59 cells
60 centrifugation
61 centrifuged fractions
62 centrifuged lipoaspirate
63 centrifuged samples
64 clonogenic
65 clonogenic efficiency
66 colony-forming ability
67 colony-forming unit fibroblast assay
68 comparison
69 concern Basic Science
70 content
71 contrary
72 counterparts
73 culture
74 cytometry analysis
75 description
76 differences
77 different donors
78 donors
79 dye assay
80 efficiency
81 endothelial progenitors
82 enrichment
83 evidence
84 experimental study
85 fat
86 fat grafting
87 fat volume
88 field
89 first study
90 fraction
91 frequency
92 frequency of CFU
93 full description
94 grafting
95 impact
96 instruction
97 journals
98 level of evidence
99 levels
100 lipoaspirate
101 manuscript
102 number
103 online Instructions
104 phenotypic profile
105 population
106 potential
107 profile
108 progenitors
109 proliferative potential
110 quality
111 ranking
112 ratings
113 review
114 review article
115 same number
116 samples
117 science
118 significant enrichment
119 stem cell population
120 stem cells
121 study
122 submission
123 subpopulations
124 surgical technique
125 table
126 technique
127 terms
128 tissue
129 trypan blue dye assay
130 unit fibroblast assay
131 viability
132 volume
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