Comparative analysis of rat mesenchymal stem cells derived from slow and fast skeletal muscle in vitro View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-10-23

AUTHORS

Etsuko Okumachi, Sang Yang Lee, Takahiro Niikura, Takashi Iwakura, Yoshihiro Dogaki, Takahiro Waki, Shunsuke Takahara, Takeshi Ueha, Yoshitada Sakai, Ryosuke Kuroda, Masahiro Kurosaka

ABSTRACT

PurposeSkeletal muscle comprises different kinds of muscle fibres that can be classified as slow and fast fibres. The purpose of this study was to compare the yield, proliferation, and multi-potentiality of rat mesenchymal stem cells (MSCs) from the tibialis anterior (TA; fast muscle) and soleus (SO; slow muscle) in vitro.MethodsThe TA and SO muscles were harvested, and isolated cells were plated. After two hours, the cells were washed extensively to remove any cell that did not adhere to the cell culture plate. The adherent cells, namely MSCs, were then cultured. Both types of MSCs were differentiated toward the osteogenic, chondrogenic and adipogenic lineages using lineage specific induction factors.ResultsThe colony-forming unit fibroblast (CFU-F) assay revealed that the SO contained significantly higher quantities of MSCs than the TA. The self-renewal capacity of MSCs derived from the TA was significantly higher at later passages (passage 9–11). Both types of MSCs exhibited similar cell surface antigens to bone marrow (BM)-derived MSCs and were positive for CD29, CD44, and CD90 and negative for CD11b, CD34, and CD45. TA-derived MSCs were superior in terms of osteogenic differentiation capacity, but there was no significant difference in chondrogenic and adipogenic differentiation capacity.ConclusionOur results demonstrated significant differences in the properties of muscle-derived MSCs from different muscle types (i.e. fast or slow muscles). The greater expandability and osteogenic differentiation ability of TA-derived MSCs suggests that fast muscle may be a better source for generating large numbers of MSCs for bone regeneration. More... »

PAGES

569-576

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00264-014-2569-6

DOI

http://dx.doi.org/10.1007/s00264-014-2569-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041701021

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25338528


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54 colony-forming unit fibroblasts
55 comparative analysis
56 culture plates
57 differences
58 different kinds
59 different muscle types
60 differentiation ability
61 differentiation capacity
62 expandability
63 factors
64 fast fibers
65 fast muscles
66 fast skeletal muscle
67 fibers
68 fibroblasts
69 good source
70 greater expandability
71 high quantities
72 hours
73 induction factor
74 kind
75 large number
76 late passages
77 lineage-specific induction factors
78 lineages
79 marrow
80 mesenchymal stem cells
81 muscle
82 muscle fibers
83 muscle types
84 muscle-derived mesenchymal stem cells
85 number
86 osteogenic differentiation ability
87 osteogenic differentiation capacity
88 passage
89 plate
90 proliferation
91 properties
92 purpose
93 quantity
94 rat mesenchymal stem cells
95 regeneration
96 results
97 self-renewal capacity
98 significant differences
99 similar cell surface
100 skeletal muscle
101 soleus
102 source
103 stem cells
104 study
105 surface
106 terms
107 tibialis anterior
108 types
109 types of MSCs
110 unit fibroblasts
111 yield
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